FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058659
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48110.4544428375898188No Hit
CTCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG40700.3844486279340184No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38570.3643288348750637No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37610.3552607591301826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32190.30406391482054185No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA31510.2976406945012511No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29100.27487604601670607No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28850.2725145679581433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28820.2722311905911158No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28210.2664691841282226No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTCACGGACACAATTGCTTGAATTGGTACCTGCAGAAGACAGGGCAGTCTC27310.2579678631173966No Hit
GTAATAGACCGCCGCGTCGTCGGGAGTCACAGACTTCAATTGCAAAGAGA27310.2579678631173966No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27110.25607868067054645No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG25510.24096522109574472No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25230.2383203656701544No Hit
GCAGAAGACAGGGCAGTCTCCACAACTCCTGATCTATTCGGGTTCCAATCGGGCCTCCGGGGTCCCTGACAGGTT24600.23236944096257625No Hit
GTTTATTACTGCATGGAAACTCTACAACTTCCGTACACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAA24510.23151930886149363No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24330.22981904465932843No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24260.2291578308029309No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24190.2284966169465333No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23740.2242459564411203No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23490.22188447838255754No Hit
CTCTACAACTTCCGTACACTTTTGGCCGGGGGACCAAGCTGGAGATCAAA23350.22056205066976242No Hit
CTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA23160.2187673273452547No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC22910.21640584928669196No Hit
GTCTTCTGCAGGTACCAATTCAAGCAATTGTGTCCGTGACTATGCAGGAG22670.21413883035047168No Hit
GTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAAAATCAGCAGAG22520.21272194351533402No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGGAAACTCTACAACTT22090.20866020125460605No Hit
GTGTAAAATCTGTGCCTGATGCACTGCCACTGAACCTGTCAGGGACCCCG21820.20610980495135828No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21480.2028981947917129No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21400.20214252181297282No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTG21130.19959212550972505No Hit
GTCTATTACTGCGCAAGAGGTATAGAGTCCCACTACTTAGACTCCTGGGGCCAGGGAAACCTGGTCTCCGTCTC19860.1875958169722262No Hit
GTTGTAGAGTTTCCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT19630.18542325715834845No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19590.18504542066897842No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19410.1833451564668132No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19240.18173935138699052No Hit
GTCCCTGACAGGTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAA18730.17692193614752247No Hit
CTCCAGGAGTGACGGACAGGGAGAGTGGAGACTGAGTCATCACAATATCC18570.1754105901900423No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC18430.17408816247724715No Hit
CCCATGGGGTGTCCTGTCACAGGTACAACTGCAGCAGTCAGGTCCAGGAC18260.17248235739742449No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA18020.1702153384612042No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17870.16879845162606655No Hit
GTCAGGGACCCCGGAGGCCCGATTGGAACCCGAATAGATCAGGAGTTGTGGAGACTGCCCTGTCTTCTGCAGGTA17800.168137237769669No Hit
CTCCTGATCTATTCGGGTTCCAATCGGGCCTCCGGGGTCCCTGACAGGTT17670.16690926917921636No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17660.16681481005687385No Hit
GGCCAAAAGTGTACGGAAGTTGTAGAGTTTCCATGCAGTAATAAACTCCA17370.16407549550894102No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACTC17050.1610528035939807No Hit
CTCCTGCATAGTCACGGACACAATTGCTTGAATTGGTACCTGCAGAAGACAGGGCAGTCTCCACAACTCCTGAT16830.15897470290244545No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16680.1575578160673078No Hit
GTCTCCACTCTCCCTGTCCGTCACTCCTGGAGAGTCGGCCTCCATCTCCT16500.1558575518651426No Hit
GTCACGGACACAATTGCTTGAATTGGTACCTGCAGAAGACAGGGCAGTCTCCACAACTCCTGATCTATTCGGGTT16140.15245702346081222No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15580.14716731260963162No Hit
AGACAGGGCAGTCTCCACAACTCCTGATCTATTCGGGTTCCAATCGGGCC15560.14697839436494659No Hit
CTCCACAACTCCTGATCTATTCGGGTTCCAATCGGGCCTCCGGGGTCCCT15360.14508921191809637No Hit
CCACTCTCCCTGTCCGTCACTCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGT15240.14395570244998626No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT14930.14102746965736843No Hit
GATCAGGAGTTGTGGAGACTGCCCTGTCTTCTGCAGGTACCAATTCAAGC14910.1408385514126834No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14870.14046071492331336No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14750.13932720545520322No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14490.13687126827429796No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14460.13658789090727044No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14340.1354543814391603No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14230.13441533109339268No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14150.13365965811465258No Hit
GCCCTGTCTTCTGCAGGTACCAATTCAAGCAATTGTGTCCGTGACTATGC13920.13148709830077485No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13800.13035358883266474No Hit
GCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTGGAACCCGAATAGATCAGGAGTTGTGGAGACTGCC13520.12770873340707442No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13430.12685860130599183No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13260.12525279622616914No Hit
AGTCAGAGCCTCCTGCATAGTCACGGACACAATTGCTTGAATTGGTACCTGCAGAAGACAGGGCAGTCTCCACAA13240.12506387798148413No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGGAAACTCTACAACTTCCGTACACTTTTGGCCGGGGGA13080.12355253202400394No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13080.12355253202400394No Hit
GTTGTGGAGACTGCCCTGTCTTCTGCAGGTACCAATTCAAGCAATTGTGTCCGTGACTATGCAGGAGGCTCTGA13000.12279685904526387No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12980.12260794080057884No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12870.12156889045481123No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12650.11949078976327598No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12630.11930187151859098No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACTCCTGGAGAGTCGGCCTCCATCTCCTGCAGGT12560.1186406576621934No Hit
GACTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGAGTGACGGACAGG12460.1176960664387683No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12440.11750714819408327No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12360.1167514752153432No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12330.11646809784831565No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGGAAACTCTACAACT12240.11561796574723306No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGACTCTCCAGGAGTGACGGACAGGGAGAGTGGAGA12230.11552350662489054No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12210.11533458838020552No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12150.11476783364615045No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12100.1142955380344379No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12070.11401216066741038No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11900.1124063555875877No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11740.11089500963010752No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11630.1098559592843399No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACTCCTGGAGAGTCGG11080.10466070755550182No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11060.10447178931081681No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11060.10447178931081681No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11040.10428287106613178No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11040.10428287106613178No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10800.10201585212991152No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10740.10144909739585645No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10670.10078788353945889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAGG200.00790006850.82933819
TGGGTTG8100.041.8370270
GGTATCA9700.039.4813041
GTATCAA25200.037.5222131
GAACCCC400.002706720734.20799634
GTCCTAT400.0028331233.8910331
GTATAAT400.0028331233.8910331
CTAACGC502.0746647E-433.8686183
ATCAACG28850.032.5185663
GTGGTAT3350.032.3735241
CAACGCA29050.032.056975
AACGCAG29400.031.9057046
TATCAAC29350.031.8582232
TCAACGC29250.031.8392814
CCTAACG553.6232942E-430.7983842
TGGTATC3650.030.6296222
AGAGTAC31000.030.58378611
ACGCAGA30950.030.198437
CGCAGAG31100.030.1616638
CGGTCCA450.005059535630.10543615
Produced by FastQC (version 0.11.5)