FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517447
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT65440.4312506466453194No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA62060.4089763925857048No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCCAGCACTCAGG61340.40423158107004725No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG56320.3711497007803238No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG51640.34030842592854976No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC49920.3289735984189234No Hit
GATTATTACTGCGCAACATGGGATAGCAGCCTGAGTGCTGGGGTCTTCGG48790.3215268803457386No Hit
ACATGGGATAGCAGCCTGAGTGCTGGGGTCTTCGGAACTGGGACCAAGGT48680.320801978586402No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC46610.30716064547888655No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG44540.29351931237137113No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC44070.2904220048542058No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43590.2872587971771008No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTTTGACAATAATAGGCGGTCCTCAGGGATTCCTGACCGATTCTCTGC41840.27572626918765536No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA39500.2603056317617683No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC39310.2590535287229142No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG38820.2558244208858695No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35440.23355016682625487No Hit
ATAATAGGCGGTCCTCAGGGATTCCTGACCGATTCTCTGCCACCAAGTCTGGCACGTCAGCCACCCTGGGCATCA32250.21252801580549435No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32060.21127591276664026No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTTTGTATCCTGGTACCAACAC31690.20883760684887182No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT31540.2078491044497765No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30990.20422459565309364No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG30990.20422459565309364No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG30250.19934798381755675No Hit
GTTCCTGGGAGGTGTTGGTACCAGGATACAAAATTATTCCCAATGTTGGA29770.19618477614045168No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTC29380.19361466990280385No Hit
CCCCAGCACTCAGGCTGCTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC28510.18788135598805097No Hit
GTACCAACACCTCCCAGGAACAGCCCCCAAACTCCTCATTTTTGACAATAATAGGCGGTCCTCAGGGATTCCTGA27670.1823457425531172No Hit
GTGCTGGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCC27190.17918253487601213No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC27150.1789189342362534No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG26680.17582162671908805No Hit
GGATAGCAGCCTGAGTGCTGGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCC26680.17582162671908805No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT26630.17549212591938962No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTATTACTGGAACTGGATCCG26110.1720653176025258No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG26060.17173581680282737No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCC26040.17160401648294799No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG25850.17035191344409392No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25790.16995651248445579No Hit
GTCTATTACTGTGCGAGAGAGCCTAGGGTCACATACACCTCTGTTCCCCA24920.16422319856970294No Hit
GTGTTGGTACCAGGATACAAAATTATTCCCAATGTTGGAGCTGCTTCCAGAGCAGGAGATGGTGACCTTCTGTC24430.16099409073265822No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT23770.1566446801766388No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGTGGC23650.15585387825736255No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA23210.15295427122001626No Hit
GTTTGGGGGCTGTTCCTGGGAGGTGTTGGTACCAGGATACAAAATTATTC22220.14643015538598714No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22200.14629835506610775No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21580.14221254514984707No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG21470.1414876433905105No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAA21180.13957653875225956No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTTTGTATCCTGGTACCAACACCTCCCAGGAACAGCCCC21110.13911523763268172No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20740.13667693171491327No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC20740.13667693171491327No Hit
GTCCTCAGGGATTCCTGACCGATTCTCTGCCACCAAGTCTGGCACGTCAG20410.13450222643690354No Hit
GTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACG20240.13338192371792887No Hit
GCCCCCAAACTCCTCATTTTTGACAATAATAGGCGGTCCTCAGGGATTCCTGACCGATTCTCTGCCACCAAGT20190.1330524229182304No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAACATGGGATAGCAGCCTGAGTGCTGGGGTCTTCGGAA19860.13087771764022071No Hit
CCTCAGGGATTCCTGACCGATTCTCTGCCACCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGA19630.12936201396160787No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG19300.12718730868359818No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC19270.1269896082037791No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG19220.12666010740408068No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT19000.12521030388540752No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18990.12514440372546784No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA18950.12488080308570909No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG18830.12409000116643282No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT18750.12356279988691532No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC18740.12349689972697563No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT18520.1220470962083025No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT18470.12171759540860407No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18260.1203336920498706No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCGTGGTGGCAGCTCCCAGATGGGTC17930.1181589867718609No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG17920.11809308661192121No Hit
CTGCTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAG17510.11539118005439399No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17460.11506167925469556No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17450.11499577909475586No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17290.11394137653572085No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17180.11321647477638427No Hit
GGGTGGCTGACGTGCCAGACTTGGTGGCAGAGAATCGGTCAGGAATCCCT17110.11275517365680647No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAACATGGGATAGCAGCCTGAGTG17070.11249157301704771No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA16940.11163487093783177No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16930.11156897077789207No Hit
GCCTATTATTGTCAAAAATGAGGAGTTTGGGGGCTGTTCCTGGGAGGTGTTGGTACCAGGATACAAAATTATTCC16660.1097896664595205No Hit
CAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAACATGGGATAGCAGC16600.10939426549988238No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG16480.1086034635806061No Hit
CTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAACATGGGATAGCAGCCTGAGTGCTGGGGTCTTCGGAA16450.10840576310078706No Hit
GTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGTGGCAGAGAATCGGTC16410.1081421624610283No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16300.10741726070169172No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16220.10689005942217421No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16180.10662645878241547No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTATTACTGGA16180.10662645878241547No Hit
GCCCAGGGTGGCTGACGTGCCAGACTTGGTGGCAGAGAATCGGTCAGGAA16020.10557205622338045No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG15890.10471535414416451No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15870.10458355382428512No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT15870.10458355382428512No Hit
TCCCTGAGGACCGCCTATTATTGTCAAAAATGAGGAGTTTGGGGGCTGTTCCTGGGAGGTGTTGGTACCAGGATA15700.10346325110531043No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15440.10174984694687854No Hit
CCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAG15320.10095904502760228No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15290.10076134454778322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3350.074.2742670
AATCGAG350.004309305559.24257370
AAATCGA250.006539294553.31883269
TCGTATG3650.050.9493240
TCTCGTA3600.049.4930338
CGTATGC3850.048.4203341
ATCTCGT3650.047.77516637
CTTATAC4050.046.4707951
GTATGCC4050.046.16359342
TGCTTGA4200.046.11494455
TAGTGAT12800.045.89756470
ATGCCGT4050.045.5266544
ACGAGAC4150.045.355522
CACGAGA4150.045.35101321
GGTATCA11000.045.2184941
TATACAC4250.045.0717473
TACATCT3850.045.0561734
TCTCCGA4200.044.8066911
CTACATC3800.044.6779433
CATCTCG3950.044.06541436
Produced by FastQC (version 0.11.5)