FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517447
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT57720.3803757231718801No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC55060.36284628062792307No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG54230.35737656735292894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52280.34452603616468974No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA52270.34446013600475006No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCCAGCACTCAGG48950.3225812829047736No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG46970.3095330512367153No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG45800.30182273252377184No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC45770.30162503204395275No Hit
GATTATTACTGCGCAACATGGGATAGCAGCCTGAGTGCTGGGGTCTTCGG44620.29404651365088863No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC44320.29206950885269795No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC43850.28897220133553264No Hit
ACATGGGATAGCAGCCTGAGTGCTGGGGTCTTCGGAACTGGGACCAAGGT40690.2681477507945912No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC40590.26748874919519433No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG39450.25997613096206984No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTTTGACAATAATAGGCGGTCCTCAGGGATTCCTGACCGATTCTCTGC38560.2541110167274376No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC37460.24686199913407192No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA37280.24567579625515754No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33840.22300614123590476No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33270.21924983211934254No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32510.21424141996392626No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTTTGTATCCTGGTACCAACAC31120.2050812977323096No Hit
GTGCTGGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCC29990.19763457965912481No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29820.19651427694015014No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29510.1944713719820198No Hit
ATAATAGGCGGTCCTCAGGGATTCCTGACCGATTCTCTGCCACCAAGTCTGGCACGTCAGCCACCCTGGGCATCA29310.19315336878322603No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG28570.1882767569476891No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTATTACTGGAACTGGATCCG28330.1866951531091366No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTC27630.18208214191335842No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26620.17542622575944994No Hit
GTACCAACACCTCCCAGGAACAGCCCCCAAACTCCTCATTTTTGACAATAATAGGCGGTCCTCAGGGATTCCTGA25970.1711427153633702No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG25710.1694293112049383No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG25420.16751820656668734No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCC24820.16356419697030605No Hit
GTCTATTACTGTGCGAGAGAGCCTAGGGTCACATACACCTCTGTTCCCCA24790.16336649649048698No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24720.16290519537090917No Hit
GGATAGCAGCCTGAGTGCTGGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCC24440.1610599908925979No Hit
GTTCCTGGGAGGTGTTGGTACCAGGATACAAAATTATTCCCAATGTTGGA24270.1599396881736232No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT23730.15638107953688005No Hit
GTGTTGGTACCAGGATACAAAATTATTCCCAATGTTGGAGCTGCTTCCAGAGCAGGAGATGGTGACCTTCTGTC23630.15572207793748316No Hit
CCCCAGCACTCAGGCTGCTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC23610.15559027761760377No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG23440.15446997489862907No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT22960.15130676722152403No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22630.14913206194351433No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAA22390.1475504581049618No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG21850.14399184946821866No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGTGGC21620.14247614578960585No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTTTGTATCCTGGTACCAACACCTCCCAGGAACAGCCCC21230.139906039551958No Hit
GTTTGGGGGCTGTTCCTGGGAGGTGTTGGTACCAGGATACAAAATTATTC21060.1387857368329833No Hit
GTCCTCAGGGATTCCTGACCGATTCTCTGCCACCAAGTCTGGCACGTCAG20930.13792903475376733No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT20790.1370064325146117No Hit
GCCCCCAAACTCCTCATTTTTGACAATAATAGGCGGTCCTCAGGGATTCCTGACCGATTCTCTGCCACCAAGT20680.13628153075527513No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20480.1349635275564814No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAGT20040.1320639205191351No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC19920.13127311859985885No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCGTGGTGGCAGCTCCCAGATGGGTC19900.13114131827997946No Hit
GTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGACTGGGGACG19700.12982331508118572No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG19410.12791221044293474No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18930.1247490027658297No Hit
GGGTGGCTGACGTGCCAGACTTGGTGGCAGAGAATCGGTCAGGAATCCCT18760.12362870004685501No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC18740.12349689972697563No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18550.12224479668812158No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18450.12158579508872469No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18430.1214539947688453No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT18200.11993829109023249No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA18150.11960879029053403No Hit
CCTCAGGGATTCCTGACCGATTCTCTGCCACCAAGTCTGGCACGTCAGCCACCCTGGGCATCACCGGACTCCAGA18070.11908158901101652No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAACATGGGATAGCAGCCTGAGTGCTGGGGTCTTCGGAA17910.11802718645198151No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17780.11717048437276556No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG17600.11598428149385118No Hit
GGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTC17460.11506167925469556No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT17340.11427087733541928No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC17300.11400727669566055No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA17260.11374367605590179No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17100.11268927349686678No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17040.11229387253722865No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16920.11150307061795238No Hit
GTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAG16880.11123946997819364No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16490.1086693637405458No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16450.10840576310078706No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16340.10768086134145048No Hit
CCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGTCCTGGGCCCAG16330.10761496118151079No Hit
CCCTGGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAACATGGGATAGCAGCCTGAGTG16100.10609925750289798No Hit
CTGCTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAG15980.1053084555836217No Hit
CTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAACATGGGATAGCAGCCTGAGTGCTGGGGTCTTCGGAA15740.1037268517450692No Hit
CTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15660.10319965046555168No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15460.10188164726675791No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG15410.10155214646705947No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTATTACTGGA15410.10155214646705947No Hit
CTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACC15260.10056364406796416No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15180.10003644278844664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)