FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165408
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55960.480175183283451No Hit
GCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAG54360.46644608583431724No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49370.4236284631648315No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT47920.4111864686015541No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTA39260.336877728658118No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG37130.3186008676789588No Hit
GATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGT36580.31388149043081903No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34010.29182912765314806No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33750.28959814931766387No Hit
CTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGT33210.28496457892858124No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTT32210.2763838930228727No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCTACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30210.25922252121145556No Hit
GTAATAATCAGCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG28780.24695214036629232No Hit
TCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCTCATAT28730.2465231060710069No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27890.2393153299102117No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27800.23854306817869794No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG27680.2375133858700129No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26330.22592945989730637No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA26010.22318364040747962No Hit
GACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAGCAGTAATAAT25880.2220681512397375No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25870.22198234438068043No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25600.21966555918613911No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25500.21880749059556823No Hit
GGATAGTTGCCTGCATATGAGCAGCAGTAATAATCAGCATCATCCTCAGC25330.2173487739915978No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25200.2162332848238557No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25160.21589005738762734No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC24870.21340165847497186No Hit
TCTCTACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG24580.21091325956231638No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24540.21057003212608802No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG24450.20979777039457423No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCACTAAG23570.20224676679755074No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23520.20181773250226528No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22600.1939235014690134No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22550.193494467173728No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATCACGAGTTTGGGGACTTTGCCTGGGTGCTGTTGGTACCAGGAGA22440.19255059172410005No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22440.19255059172410005No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC22040.18911831736181664No Hit
GGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCG21660.18585765671764737No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20830.17873568741590928No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20180.17315824157719872No Hit
GTCTTCTCTACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19920.17092726324171448No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19870.17049822894642908No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19810.16998338779208655No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19740.16938273977868695No Hit
CTTCTCTACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18750.16088786073203548No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAG18550.15917172355089376No Hit
CAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA18540.15908591669183667No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC18490.15865688239655126No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT18420.15805623438315164No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGC18410.15797042752409457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18090.15522460803426782No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18090.15522460803426782No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17780.15256459540349818No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17650.15144910623575608No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA17420.14947554847744307No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17370.14904651418215767No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTC17070.14647230841044512No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16700.14329745462533294No Hit
GTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCA16640.14278261347099042No Hit
GACCATCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCT16510.1416671243032483No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16320.14003679398116367No Hit
ACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGC16240.139350339108707No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGG16240.139350339108707No Hit
GCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCC15810.1356606441692523No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15520.13317224525659682No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15490.13291482467942556No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCTACGGTGCTCCCTTCATGCGTGACCTGGCAG15450.13257159724319723No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT15230.13068384634394134No Hit
GTCCCCAAACTCGTGATTTATGATGTCACTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT14850.1274231856997721No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14700.1261360828139158No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGGCCTCCCTGACCATCTCTGGCCTCCAGG14700.1261360828139158No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT14590.12519220736428785No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14330.12296122902880363No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14310.12278961531068948No Hit
GCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTG14110.12107347812954777No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13790.11832765863972103No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13740.1178986243444356No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13690.11746959004915015No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT13690.11746959004915015No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13680.11738378319009309No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGA13320.11429473626403801No Hit
GGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTC13260.11377989510969548No Hit
TCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC13220.11343666767346715No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13150.11283601966006755No Hit
ATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTT13080.11223537164666796No Hit
GTTATAACCACCAACATCACCGCTGGTTCCAGTGCAGGAGATGGTGACTG12940.11103407561986875No Hit
CTCCCTGACCATCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATCC12850.11026181388835497No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC12840.11017600702929789No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC12680.10880309728438452No Hit
GCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCC12640.10845986984815618No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12600.10811664241192785No Hit
CAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCACTAAGCGGCCCTCAGGGGTCCCTGATCG12490.1071727669622999No Hit
GTCTGGCATCACGGCCTCCCTGACCATCTCTGGCCTCCAGGCTGAGGATG12350.1059714709355007No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTC12170.10442694747247315No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC12160.10434114061341608No Hit
GTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAA12160.10434114061341608No Hit
GGCTGAGGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATC12130.10408372003624482No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12070.1035688788819023No Hit
GAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATC12050.10339726516378814No Hit
CCTGAGGGCCGCTTAGTGACATCATAAATCACGAGTTTGGGGACTTTGCC12020.10313984458661689No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG11980.10279661715038853No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11960.10262500343227438No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACAT11930.10236758285510311No Hit
GGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGA11700.10039402509679014No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11690.10030821823773305No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC11680.10022241137867596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG353.687128E-758.1684232
CTAACGC452.095032E-645.216873
GTATCAA29850.040.7248881
GGTATCA11450.040.035891
TACGTAT750.001488667638.63557470
TATCAAC34950.034.756812
ATCAACG36950.032.76543
TCAACGC37000.032.7169154
CAACGCA37650.032.242145
CTTATAG851.398148E-731.9356042
AACGCAG38450.031.8358676
TGGTATC4500.030.915442
CGCAGAG40400.030.4670968
ACGCAGA40650.030.2797247
GTGGTAT4600.030.2654571
GCAGAGT41300.029.8031659
AGAGTAC41100.029.69941311
CAGAGTA41650.029.6328610
AATGGTA4050.028.61894270
GGTACGT550.00680043828.33740668
Produced by FastQC (version 0.11.5)