FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559641
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC87610.5617318344413874No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC58890.3775868933940567No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCCCCGCCGAACACTCT56700.36354520046600464No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG52110.334115350904471No Hit
GACCTAGGACGGTCAGTCTGGTCCCCCCGCCGAACACTCTCTGATTACCA48710.31231546234037194No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44500.2853220709124728No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG44050.28243679154369494No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT43150.27666623280613933No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37360.23954230492786482No Hit
GTAATAAAGAGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGAGAGA36940.2368493775170055No Hit
GGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCC36790.23588761772741293No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36430.23357939423239066No Hit
CCTATGAGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGAT36000.2308223495022252No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC35520.22774471817552885No Hit
GCACAGGGGCCTGGCCTGACCTCTGCTGGTACCAATAAGCATATTTCCTT35460.2273600142596918No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCCC33970.21780653368307196No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG33880.21722947780931637No Hit
GAGTAACAGTGGTAGTCAGCCTCATCCTCCACCTGGGCCCCACTGATAGT32300.20709894135894094No Hit
CTTTATTACTGTGCGAGGGTCGCTAACAATAAGGATGGTAGTCGCAATTA31810.2039571927129384No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31530.20216190777236556No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31490.20190543849514087No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31070.1992125110842816No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATCT30720.1969684049085655No Hit
GCTCAGGGACAGTGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGAT30420.19504488532938027No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29890.1916466674061531No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29850.1913901981289284No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29410.18856903607945674No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29260.18760727628986415No Hit
CTGTTACTCAGTAGACGGCAGTGGTAATCAGAGAGTGTTCGGCGGGGGGACCAGACTGACCGTCCTAGGTCAGCC29070.18638904722304686No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28010.17959261137659244No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT27980.1794002594186739No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27600.1769638012850393No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27580.17683556664642697No Hit
GTCTATGGTGGGTCCTTCAGTGATTACTACTGGAGCTGGGTCCGCCAGCC26850.17215500233707628No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATCTATTGCCAAGG26780.17170618110193306No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26190.16792325926286883No Hit
ACCTAGGACGGTCAGTCTGGTCCCCCCGCCGAACACTCTCTGATTACCAC25650.16446092402033544No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAG25450.16317857763421198No Hit
GTCTGAGAGTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTC25340.16247328712184408No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25330.1624091698025379No Hit
GTAGACGGCAGTGGTAATCAGAGAGTGTTCGGCGGGGGGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCC25050.16061388486196504No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24500.15708743230012548No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24490.1570233149808193No Hit
GGCCTGGCCTGACCTCTGCTGGTACCAATAAGCATATTTCCTTGGCAATAGATCTCCAGAGCAGGTGATCCTGGC24380.1563180244684514No Hit
GTCTGGTCCCCCCGCCGAACACTCTCTGATTACCACTGCCGTCTACTGAGTAACAGTGGTAGTCAGCCTCATCCT24020.15400980097342915No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23960.15362509705759209No Hit
GGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG23670.15176569479771307No Hit
GACTACCACTGTTACTCAGTAGACGGCAGTGGTAATCAGAGAGTGTTCGG23330.14958570594130316No Hit
TCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC22600.14490514163195248No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22490.14419985111958458No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG22280.1428533874141549No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC22090.14163515834733764No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22070.14150692370872528No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22020.14118633711219442No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21540.13810870578549808No Hit
GTACCAGCAGAGGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACA21250.13624930352561904No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20800.13336402415684123No Hit
GTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCT20650.1324022643672486No Hit
AGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATCTATTGC20610.13214579509002392No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20550.13176109117418688No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20540.13169697385488072No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20410.13086344870390046No Hit
GTGGTAGTCAGCCTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG20290.13009404087222637No Hit
GCCGTCTACTGAGTAACAGTGGTAGTCAGCCTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCA20170.12932463304055228No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCCCCGCCGAACA20060.12861934252818438No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20030.12842699057026585No Hit
GACCAGCACAGGGGCCTGGCCTGACCTCTGCTGGTACCAATAAGCATATT19470.12483642068912013No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT19370.12419524749605838No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19250.12342583966438431No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19180.12297701842924108No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18730.12009173906046328No Hit
GGATCACCTGCTCTGGAGATCTATTGCCAAGGAAATATGCTTATTGGTACCAGCAGAGGTCAGGCCAGGCCC18470.11842468875850275No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18390.11791175020405337No Hit
GGATGAGGCTGACTACCACTGTTACTCAGTAGACGGCAGTGGTAATCAGAGAGTGTTCGGCGGGGGGACCAGA18330.11752704628821634No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18280.11720645969168544No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC18150.1163729345407052No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18130.11624469990209287No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17910.11483411887735703No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17590.11278236465955949No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG17270.11073061044176193No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17200.1102817892066187No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA17030.10919179477841376No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16850.10803768303090262No Hit
GCCTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCACTGTCCC16790.10765297911506558No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16620.10656298468686062No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG16620.10656298468686062No Hit
GGCCTGACCTCTGCTGGTACCAATAAGCATATTTCCTTGGCAATAGATCT16610.10649886736755446No Hit
CTGATAGTCAAGGTGGCCACTGTCCCTGAGCTGGAGCCAGAGAATCTCTC16560.10617828077102359No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16510.10585769417449271No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16500.10579357685518655No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC16470.10560122489726802No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16370.10496005170420629No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16130.10342123604085814No Hit
GCTCTGGAGATCTATTGCCAAGGAAATATGCTTATTGGTACCAGCAGAGG16110.10329300140224577No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16110.10329300140224577No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16030.10278006284779638No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15900.10194653769681614No Hit
CTGCTGGTACCAATAAGCATATTTCCTTGGCAATAGATCTCCAGAGCAGGTGATCCTGGCCGTTTGTCCTGGGGA15890.10188242037750994No Hit
GGAAATATGCTTATTGGTACCAGCAGAGGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGAC15760.1010488952265297No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAGGCTGACTACCACTGTTACT15670.10047183935277414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG250.001317565979.8785270
TACGTAT503.8288854E-666.5654370
GTGGTAT2650.048.578551
GGTATCA9900.044.142951
ACCGATC1100.040.09061427
TGGTATC3100.039.339942
GGTACCG350.001474468938.7154926
TACTGAT13350.038.39354770
GACCGAT1150.038.32539426
TCTATAC451.11536225E-437.6400643
GTATCAA25350.037.2849041
GTACGTA601.3421576E-436.473769
TAATAGG400.002598284734.49544540
CTCGTTT601.3637104E-534.42239439
AGTATCG400.002732840634.14207534
CCATACA400.002839674533.8760574
TATCAAC28400.033.1603662
GAATACG1350.032.6192979
ATCAACG29050.032.41843
TCAACGC29300.032.2574044
Produced by FastQC (version 0.11.5)