FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559641
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC87580.561539482483469No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC56090.3596340439883281No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCCCCGCCGAACACTCT54160.34725940136223654No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47910.30718607679587806No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG45240.2900667525411296No Hit
GACCTAGGACGGTCAGTCTGGTCCCCCCGCCGAACACTCTCTGATTACCA44290.2839756072070432No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT41750.26768980810327503No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG38600.24749285252183031No Hit
GTAATAAAGAGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGAGAGA37570.24088876863329448No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36710.23537467917296354No Hit
GGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCC34210.2193453493464201No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33940.21761418172515343No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC33760.2164600699776423No Hit
CTTTATTACTGTGCGAGGGTCGCTAACAATAAGGATGGTAGTCGCAATTA33380.2140236118440077No Hit
CCTATGAGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGAT33230.21306185205441508No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31910.20459836590600017No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG31820.2040213100322446No Hit
GCACAGGGGCCTGGCCTGACCTCTGCTGGTACCAATAAGCATATTTCCTT31390.20126426530207914No Hit
GAGTAACAGTGGTAGTCAGCCTCATCCTCCACCTGGGCCCCACTGATAGT31250.20036662283179268No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31210.20011015355456802No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30270.19408312553978768No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCCC30050.19267254451505186No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATCT29900.19171078472545927No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29600.18978726514627403No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT29310.18792786288639501No Hit
CTGTTACTCAGTAGACGGCAGTGGTAATCAGAGAGTGTTCGGCGGGGGGACCAGACTGACCGTCCTAGGTCAGCC28590.1833114158963505No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28300.18145201363647145No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28140.1804261365275727No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27240.17465557779001706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27190.1743349911934862No Hit
GCTCAGGGACAGTGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGAT26770.1716420637826269No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCCCCGCCGAACA26740.17144971182470836No Hit
GTCTATGGTGGGTCCTTCAGTGATTACTACTGGAGCTGGGTCCGCCAGCC26660.170936773270259No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAG26420.16939795760691082No Hit
GTCTGAGAGTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTC26240.1682438458593997No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26210.1680514939014812No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26200.16798737658217502No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25450.16317857763421198No Hit
GTCTGGTCCCCCCGCCGAACACTCTCTGATTACCACTGCCGTCTACTGAGTAACAGTGGTAGTCAGCCTCATCCT25310.16228093516392555No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24560.15747213621596254No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATCTATTGCCAAGG24430.15663861106498225No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24020.15400980097342915No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC23990.15381744901551062No Hit
ACCTAGGACGGTCAGTCTGGTCCCCCCGCCGAACACTCTCTGATTACCAC23940.15349686241897975No Hit
GTAGACGGCAGTGGTAATCAGAGAGTGTTCGGCGGGGGGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCC23590.15125275624326368No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23380.14990629253783402No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23000.14746983440419942No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22840.14644395729530066No Hit
GGCCTGGCCTGACCTCTGCTGGTACCAATAAGCATATTTCCTTGGCAATAGATCTCCAGAGCAGGTGATCCTGGC22800.14618748801807596No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22690.14548219750570804No Hit
GACTACCACTGTTACTCAGTAGACGGCAGTGGTAATCAGAGAGTGTTCGG22500.14426396843889075No Hit
GTGGTAGTCAGCCTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGG22190.14227633154039937No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21220.1360569515677005No Hit
TCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC20800.13336402415684123No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20290.13009404087222637No Hit
GCCGTCTACTGAGTAACAGTGGTAGTCAGCCTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCA19860.1273369961420609No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19620.12579818047871275No Hit
GGACCAGACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG19600.12566994584010038No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19330.12393877821883369No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19270.12355407430299666No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA19250.12342583966438431No Hit
GGATCACCTGCTCTGGAGATCTATTGCCAAGGAAATATGCTTATTGGTACCAGCAGAGGTCAGGCCAGGCCC19210.12316937038715961No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19190.12304113574854728No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18970.12163055472381144No Hit
GTACCAGCAGAGGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACA18940.12143820276589291No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18860.12092526421144352No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18830.120732912253525No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18580.11912997927087067No Hit
GTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCT18500.11861704071642128No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18350.11765528092682867No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18330.11752704628821634No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG18000.1154111747511126No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17980.11528294011250026No Hit
GACCAGCACAGGGGCCTGGCCTGACCTCTGCTGGTACCAATAAGCATATT17920.1148982361966632No Hit
GGATGAGGCTGACTACCACTGTTACTCAGTAGACGGCAGTGGTAATCAGAGAGTGTTCGGCGGGGGGACCAGA17600.11284648197886565No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17510.11226942610511009No Hit
GGCCTGACCTCTGCTGGTACCAATAAGCATATTTCCTTGGCAATAGATCT17460.11194883950857923No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17400.11156413559274217No Hit
AGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATCTATTGC17290.11085884508037426No Hit
GCCTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCACTGTCCC17160.11002531992939402No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17150.10996120261008785No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17070.10944826405563846No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17060.10938414673633226No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC16940.10861473890465818No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16920.10848650426604585No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16890.10829415230812732No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGTCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG16750.10739650983784088No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTCTGGTCCCCCCGCCGAACACT16740.10733239251853471No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG16690.10701180592200385No Hit
CTGCTGGTACCAATAAGCATATTTCCTTGGCAATAGATCTCCAGAGCAGGTGATCCTGGCCGTTTGTCCTGGGGA16350.10483181706559394No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16310.10457534778836924No Hit
CTCTCTGATTACCACTGCCGTCTACTGAGTAACAGTGGTAGTCAGCCTCA16260.10425476119183838No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16100.1032288840829396No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15770.10111301254583586No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15600.10002301811763091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3150.045.1181141
GGTATCA10150.041.6731381
GTCTTAA603.3888864E-739.4783551
TGGTATC3650.037.9995042
TACTGAT13400.037.20900370
GTATCAA25950.035.468581
GTTAGTC400.002847947233.855969
GGCAACG16900.033.14035470
ACGAACC552.838681E-432.10821552
TATCAAC29100.031.3875682
TTAATCG553.3772213E-431.17122337
CATAACC450.0044877930.85352344
ATAGCGG603.9990267E-430.28510763
ATCAACG30200.030.1303653
TCTACCG450.005087039430.072965
TCAACGC30500.029.9449064
CAACGCA30850.029.609925
AACGCAG31500.029.0054346
TCGTTGA605.368036E-428.80011641
AATCGTT605.500035E-428.68080939
Produced by FastQC (version 0.11.5)