FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526673
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21700.4120203617804596No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18910.3590463152658291No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17130.32524925333176374No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG15010.284996572826023No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14930.28347760375033465No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13750.2610728098839318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13450.25537667585010054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12350.234490851059386No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12250.2325921397147756No Hit
GTAATAGACGGCCGTGTCTTCAGATCTCAGGCTGGTCACCTCCATGTAGG12220.23202252631139247No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACCATTTAAATTGGTATCAGCAGAAA12010.2280352324877106No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11530.21892141803358062No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11520.21873154689911958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10960.20809876336930125No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10770.20449121181454147No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA10750.2041114695456194No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10700.20316211387331418No Hit
GTCTATTACTGTGCGAGAGATGGAGAGACTCTTGGATTCGGGGGGTACTATTTTGAGTACTGGGGCCAGGGAACC10550.2003140468563986No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10120.1921495880745738No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10090.19157997467119067No Hit
ATATTACTGTCAACATTTTGATAGCCTCCCGCTCACTTTCGGCGGAGGGA10070.19120023240226858No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9630.18284590248598276No Hit
ACATATTACTGTCAACATTTTGATAGCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA9500.18037757773798924No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9410.17866873752783985No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8960.170124536477093No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8760.16632711378787218No Hit
CATCAAGATTCAGTGGAACTGGATCAGGGACATATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATATTG8760.16632711378787218No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8710.165377758115567No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATATGTCCCTGATCCAGTTCCA8680.16480814471218386No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8560.16252969109865134No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8450.16044110861957991No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATATGTCCCTGATCCAGTTCCA8310.1577829127371253No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7620.14468180445931347No Hit
ATCTTGATGAGACCCCTGTTTTCAAGTTGGATGCGCCGTAGATCAGGAGCTTAGGGGCTTTCCCTAGTTTCTG7530.1429729642491641No Hit
AGTCAGGACATTAGCAACCATTTAAATTGGTATCAGCAGAAACTAGGGAA7410.1406945106356316No Hit
GGACATTAGCAACCATTTAAATTGGTATCAGCAGAAACTAGGGAAAGCCCCTAAGCTCCTGATCTACGGCGCATC7380.14012489723224847No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7280.13822618588763808No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7280.13822618588763808No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7090.13461863433287827No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6890.13082121164365745No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT6850.13006172710581326No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6840.12987185597135223No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6840.12987185597135223No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG6780.128732629164586No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC6770.12854275803012497No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6700.12721366008889767No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC6680.12683391781997558No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6480.12303649513075475No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6340.12037829924830018No Hit
GATCTACGGCGCATCCAACTTGAAAACAGGGGTCTCATCAAGATTCAGTGGAACTGGATCAGGGACATATTTTA6330.12018842811383913No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6220.11809984563476768No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6170.11715048996246248No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT6070.11525177861785206No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6070.11525177861785206No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG6060.11506190748339101No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC6030.1144922940800079No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT6000.11392268067662477No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6000.11392268067662477No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6000.11392268067662477No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5990.11373280954216373No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5920.11240371160093644No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5790.1099353868529429No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5770.10955564458402083No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5760.10936577344955979No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA5730.10879616004617666No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5700.10822654664279353No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC5630.10689744870156626No Hit
CCCCTGGACAAGGGCTTGAGTGGATTGGAAAGATCGTCCCTGTCCTTGGT5530.10499873735695583No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5520.1048088662224948No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5490.10423925281911167No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5450.1034797682812675No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5440.10328989714680646No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT5440.10328989714680646No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5410.10272028374342333No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5390.10234054147450126No Hit
GAACTGGATCAGGGACATATTTTACTTTCACCATCAGCAGCCTGCAGCCT5370.10196079920557917No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5360.10177092807111814No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA5350.10158105693665709No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5340.10139118580219605No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACATTTTGATAGCCTCCCGC5310.10082157239881293No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5280.1002519589954298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCC150.002289507569.49103543
CGCCAGT150.002386569568.7673138
CGCCCAA200.00789127150.8400920
CGCGGTA4300.040.2903270
AATGGTA2000.039.98039670
GTGGTAT1550.039.3264241
GTATCAA12400.037.9609221
AGCACAA3200.035.3993170
ACGGGTA1103.45608E-1133.87729316
TTAGGAC502.0782379E-433.854783
GCCTGAC2050.033.0353247
TATCAAC14350.032.56032
CGACGGG450.004241687231.20725649
CGGGTAG450.005046228430.11887417
AACCGAC450.005062666330.098857
CTCGTCT450.00506737130.09313413
TCAACGC15200.030.0712434
ATCAACG15200.030.0683883
CAACGCA15300.029.8775355
TTCGCGG500.005271890729.85002167
Produced by FastQC (version 0.11.5)