FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788743
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGTAGATACA57420.32100754552219074No Hit
CTATAGGAGAGGAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC49450.2764511167898351No Hit
GTCCTGGACCCCTGCCCGGTTCTCAGGTTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC44020.2460946038642779No Hit
CTCCTATAGTGGTGCTCGGCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC42010.23485766261559093No Hit
GTCTATTACTGTGCGAGAGGTCCCTTCGTTGTTATAGAATTCTGGGGCCG40660.22731046326945792No Hit
GTCCAGGACTGTTTGTTGCTGACATCATAAATCAATGTTCTGGGGGCTTG39850.22278214366177815No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37960.21221606457719192No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA36840.2059546843789186No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34020.19018942352255186No Hit
CCACTATAGGAGAGGAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG31140.1740887315841348No Hit
GCCTGAGGATGAGGCTGAATATTACTGCTTCCTCTCCTATAGTGGTGCTCGGCTCTGGGTGTTCGGCGGAGGGAC30100.16827459282859528No Hit
GCACTGGACCTGTCACCAGTGGTCATTATCCCTACTGGCTCCAGTTGAAG30010.16777144620551973No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29900.16715648922176074No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTC29430.16452894574569965No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC28780.16089510902348744No Hit
GTTCTCAGGTTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG27950.15625497905512417No Hit
GAATATTACTGCTTCCTCTCCTATAGTGGTGCTCGGCTCTGGGTGTTCGG27800.15541640134999832No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT25970.14518575334746242No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA25930.14496213262609553No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25440.14222277878935097No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25220.14099286482183299No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24490.13691178665688697No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGAGCCGAGCA24250.13557006232868554No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23840.13327794993467482No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23470.13120945826203095No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23320.13037088055690504No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23250.12997954429451297No Hit
GTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG22750.12718428527742665No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22560.12612208685093387No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21960.12276777603043032No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21920.12254415530906342No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21860.12220872422701305No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21410.11969299111163538No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21380.1195252755706102No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC21230.11868669786548432No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21030.1175685942586498No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20810.11633868029113181No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20620.11527648186463901No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20600.11516467150395557No Hit
GTTGAAGCCTGGCCAAGCCCCCAGAACATTGATTTATGATGTCAGCAACAAACAGTCCTGGACCCCTGCCCGGTT20250.11320799019199515No Hit
CCTGAGAACCGGGCAGGGGTCCAGGACTGTTTGTTGCTGACATCATAAATCAATGTTCTGGGGGCTTGGCCAGG20010.11186626586379375No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19890.11119540369969304No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19830.11085997261764267No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19810.11074816225695923No Hit
GCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT19770.11052454153559232No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19750.11041273117490887No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19680.11002139491251678No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA19330.10806471360055638No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19220.10744975661679738No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTCCAGGACTGTTTGTTG19060.10655527373132977No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTCCAG18920.1057726012065456No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18640.10420725615697726No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18520.10353639399287655No Hit
CCTATAGTGGTGCTCGGCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC18430.10303324736980102No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18290.10225057484501686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC5650.044.93752322
CACGAGA5650.044.93501321
TCGCGTA851.8189894E-1244.42834538
CGTATGC4250.043.8743641
CGAGACG5900.043.0394123
CGCGTAT803.6379788E-1143.00031739
CGCTCAT6100.042.829428
ATGAATC5300.041.1436233
ATCGCGT1000.041.12256237
AATCGCG1250.041.03892536
CTTATAC6200.040.9419751
ACGCTCA6300.040.90823727
GCGTATG856.730261E-1140.54923240
ATGCCGT4150.040.14642744
ATCTCGT3350.039.89502337
CGAGCCC6400.039.6592115
CATGAAT6300.039.4425832
TCGTATG4000.038.77520440
CCCACGA6650.038.6836619
GTATGCC5100.038.66818242
Produced by FastQC (version 0.11.5)