FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788743
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51430.28752034249749686No Hit
GTCCTGGACCCCTGCCCGGTTCTCAGGTTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC47060.2630897786881626No Hit
GTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGTAGATACA46190.2582260279984324No Hit
CTATAGGAGAGGAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC44660.2496725354061483No Hit
CTCCTATAGTGGTGCTCGGCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC39750.2222230918583609No Hit
GTCTATTACTGTGCGAGAGGTCCCTTCGTTGTTATAGAATTCTGGGGCCG36280.20282399427978196No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35670.19941377827893667No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35360.19768071768834317No Hit
GTCCAGGACTGTTTGTTGCTGACATCATAAATCAATGTTCTGGGGGCTTG35090.19617127781911656No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA29840.16682105813971038No Hit
GCCTGAGGATGAGGCTGAATATTACTGCTTCCTCTCCTATAGTGGTGCTCGGCTCTGGGTGTTCGGCGGAGGGAC29180.1631313162371565No Hit
GTTCTCAGGTTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG27670.15468963400555585No Hit
GCACTGGACCTGTCACCAGTGGTCATTATCCCTACTGGCTCCAGTTGAAG27370.1530124785953041No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA27240.15228571125086166No Hit
CCACTATAGGAGAGGAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG27190.152006185349153No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26810.14988178849616743No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTC26560.14848415898762427No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26500.1481487279055739No Hit
GAATATTACTGCTTCCTCTCCTATAGTGGTGCTCGGCTCTGGGTGTTCGG25860.14457079636370346No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT24420.1365204503944949No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23930.13378109655775033No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23410.1308740271799806No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22720.1270165697364015No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22340.12489217288341589No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22300.12466855216204899No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC21870.12226462940735479No Hit
GTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG21110.1180158357013836No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21020.11751268907830806No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20600.11516467150395557No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20550.11488514560224694No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20430.11421428343814623No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20150.1126489383885779No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20080.11225760212618582No Hit
GTTGAAGCCTGGCCAAGCCCCCAGAACATTGATTTATGATGTCAGCAACAAACAGTCCTGGACCCCTGCCCGGTT19830.11085997261764267No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19380.108344239502265No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19340.1081206187808981No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19320.10800880842021464No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19020.10633165300996286No Hit
GCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT19000.1062198426492794No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18950.10594031674757078No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18700.10454268723902763No Hit
CCTGAGAACCGGGCAGGGGTCCAGGACTGTTTGTTGCTGACATCATAAATCAATGTTCTGGGGGCTTGGCCAGG18690.1044867820586859No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18420.1029773421894593No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTCCAG18210.10180333340228306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)