FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220797
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTATACAGCCGTGTCTGCGGCGGTCACAGACCTCATCTTCAAGGAGA10940.4954777465273532No Hit
GAGTTACAGTCACCCTTTGACTTTTGGCCAGGGGACCAAACTGGAGATCAGGCGAACTGTGGCTGCACCAT9920.44928146668659447No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8280.37500509517792363No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT7440.33696110001494584No Hit
GACCAGGGACAGCCCCTAAACTCCTGATCTATGGCGCATCCAGTTTGCAA7360.33333786238037655No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7120.3224681494766686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6300.2853299637223332No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6140.2780834884531946No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5790.2622318238019538No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5490.24864468267231893No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5450.24683306385503428No Hit
GACTTCACTCTCACCATCAGCAGCCTTCACCCTGACGATTTTGCAACTTACTTCTGTCAACAGAGTTACAGTCAC5270.23868077917725333No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5260.23822787447293214No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4980.22554654275193955No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT4910.2223762098216914No Hit
CTGTAACTCTGTTGACAGAAGTAAGTTGCAAAATCGTCAGGGTGAAGGCTGCTGATGGTGAGAGTGAAGTCTGTC4910.2223762098216914No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4680.21195940162230467No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4660.21105359221366232No Hit
GAGTGAAGTCTGTCCCAGATCCACCGCCAGTGAACCTTGATGGGACCCCA4440.20108968871859673No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG4390.1988251651969909No Hit
GTATACTACTGTGCGAGACACTTGGATGTGATGACTGACACTGGGTTTCA4380.19837226049266976No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4370.19791935578834857No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4360.19746645108402744No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4280.1938432134494581No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4190.18976707111056762No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4140.18750254758896182No Hit
GTCCCTGGTCTCTGCTGATACCAATTTAAAGAATTGAAGATGTTCTGACTTGCCCGGCAAGTGATGGTGACT4120.1865967381803195No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4000.1811618817284655No Hit
GCCTTCACCCTGACGATTTTGCAACTTACTTCTGTCAACAGAGTTACAGTCACCCTTTGACTTTTGGCCAGGGGA3940.17844445350253854No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC3910.17708573938957506No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3850.17436831116364807No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3830.17346250175500574No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCCTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTCAAAGGGTGA3800.17210378764204226No Hit
GTCTCTGGTGACTCCATCAGCACTAACAGTCACTACTGTGGCTGGATCCG3680.16666893119018827No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3630.16440440766858244No Hit
GCCCCTAAACTCCTGATCTATGGCGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCGGTGGAT3610.16349859825994012No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC3570.16168697944265548No Hit
GTGCAGCCACAGTTCGCCTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTC3570.16168697944265548No Hit
GTCTACTACAACCCCTCTCTCAAGAGTCGAGTCACCATTTCCGTAGACAC3500.15851664651240732No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3490.15806374180808616No Hit
CCCTTTGACTTTTGGCCAGGGGACCAAACTGGAGATCAGGCGAACTGTGG3470.15715793239944384No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3440.15579921828648033No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT3400.15398759946919569No Hit
GTTTAGGGGCTGTCCCTGGTCTCTGCTGATACCAATTTAAAGAATTGAAG3340.1512701712432687No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC3320.15036436183462637No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3200.1449295053827724No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3100.14040045833956077No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG3090.1399475536352396No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTTCACCCTGACGATT3070.1390417442265973No Hit
CTCCTGATCTATGGCGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCGGTGGATCTGGGACAGA3060.13858883952227613No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG3040.1376830301136338No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3030.13723012540931265No Hit
GTCCCAGATCCACCGCCAGTGAACCTTGATGGGACCCCACTTTGCAAACT2950.13360688777474333No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2940.13315398307042217No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGAAGTGACTTCCAGATGACCCAGTC2940.13315398307042217No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT2910.13179526895745866No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT2840.1286249360272105No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2820.12771912661856819No Hit
GGGTGAAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACCGCC2820.12771912661856819No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT2810.12726622191424702No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2800.12681331720992586No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC2770.12545460309696235No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA2740.12409588898399887No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG2730.12364298427967771No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT2720.12319007957535655No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT2700.12228427016671421No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT2700.12228427016671421No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG2690.12183136546239305No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT2690.12183136546239305No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC2680.12137846075807189No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC2640.11956684194078723No Hit
GATCAGGAGTTTAGGGGCTGTCCCTGGTCTCTGCTGATACCAATTTAAAG2640.11956684194078723No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC2580.11684941371486025No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG2580.11684941371486025No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2570.11639650901053909No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT2530.11458489019325444No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG2530.11458489019325444No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC2510.11367908078461211No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG2500.11322617608029095No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA2490.11277327137596978No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG2480.11232036667164862No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC2470.11186746196732746No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC2450.11096165255868513No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC2450.11096165255868513No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATCTTCAATTCTTTAAATTGGTATCAGCAGAGA2450.11096165255868513No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC2430.1100558431500428No Hit
CACTTGCACTGTCTCTGGTGACTCCATCAGCACTAACAGTCACTACTGTG2430.1100558431500428No Hit
GATCTATGGCGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCGGTGGATCTGGGACAGACTTCA2420.10960293844572164No Hit
GCAGAGACCAGGGACAGCCCCTAAACTCCTGATCTATGGCGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT2400.10869712903707932No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC2380.10779131962843698No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA2380.10779131962843698No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG2360.10688551021979466No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2340.10597970081115234No Hit
GTTCTGACTTGCCCGGCAAGTGATGGTGACT2330.10552679610683116No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT2320.10507389140251No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC2320.10507389140251No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC2300.10416808199386768No Hit
GCTGTATACTACTGTGCGAGACACTTGGATGTGATGACTGACACTGGGTT2290.10371517728954652No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA2290.10371517728954652No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC2280.10326227258522534No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAAGTGACTT2250.10190355847226186No Hit
GTATACAGCCGTGTCTGCGGCGGTCACAGACCTCATCTTCAAGGAGAACT2230.10099774906361952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGT150.001607502375.95367
ATTAAGT150.001818125573.63325565
TAGGCAA300.002923723865.3016670
GCCGTCT900.057.9497246
TACGGAA2600.057.76685370
CGTATGC900.057.4817341
TCGTATG900.057.3890440
TTAAGTT200.005396140355.95538766
GTATGCC950.054.569742
CTTGAAA1050.054.4128557
AAGTATT200.006105460754.2363961
CACGAGA1000.054.2068921
GGGTAAG200.00641504953.5626557
ATACACA1100.052.33524
ATGCCGT1000.051.973444
TATGCCG1000.051.88920243
TGCTTGA1100.051.70485355
TATACAC1050.051.602223
AGACGCG1000.050.86507425
CGAGACG1000.050.8304823
Produced by FastQC (version 0.11.5)