FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785178
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGGACTGAGGAGTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT33530.42703692665866844No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC28430.36208350208487755No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAGCATTGTCAG27810.354187203411201No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGAGGTTGCTGCTATGATAAGACTGA23890.30426221824860095No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22190.282611076724004No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCCGTCGATGGAGCCA22190.282611076724004No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAGCATTGTCAGCCACTATGTG20710.2637618476320019No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18850.2400729516109723No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18740.23867199539467482No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18110.23064833706497126No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGAGGTTGCTGCTATGATAAGACTGACAGTAGTAGT18010.22937474050470086No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16930.2156198976537804No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC16500.21014343244461767No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16260.20708680069996868No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15910.20262921273902224No Hit
GACTACTACTGTCAGTCTTATCATAGCAGCAACCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT15840.20173769514683296No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15370.19575179131356202No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15230.19396875612918343No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15200.1935866771611023No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14800.18849229092002068No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14620.18619981711153394No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14240.1813601501825064No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14230.18123279052647936No Hit
GTAGTAGTCAGCCTCGTCCTCACTCCTCAGTCCAGAGATGGTGAGGGAGG14140.18008655362223597No Hit
ATCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACGGC14020.17855823774991147No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13930.17741200084566813No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13400.17066193907623495No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13160.16760530733158596No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13060.16633171077131556No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12960.16505811421104513No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12730.1621288421224232No Hit
CTACTACTGTCAGTCTTATCATAGCAGCAACCTCTGGGTGTTCGGCGGAG12280.15639765760120636No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG12100.1541051837927196No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT12000.15283158723244922No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11820.15053911342396248No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11640.1482466396154757No Hit
GTAATATACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTGTGCTCGTGGACATGTCTGTGGT11490.1463362447750701No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11420.14544472718288082No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11170.1422607357822048No Hit
GAGTTGGAGGAGCCGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA10860.13831258644536654No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10810.13767578816523132No Hit
CAGTAGTAGTCAGCCTCGTCCTCACTCCTCAGTCCAGAGATGGTGAGGGA10770.13716634954112317No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10640.13551067401277164No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10590.13487387573263643No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT10560.1344917967645553No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10550.13436443710852825No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10440.1329634808922308No Hit
GTGCAATGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTAATCTA10170.1295247701795007No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10050.1279964543071762No Hit
CTCCTGCACCGGCAGCAGTGGCAGCATTGTCAGCCACTATGTGCAATGGTACCAGCAGCGCCCGGGCAGTGCCCC9970.12697757705895987No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9810.12493982256252723No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9500.12099167322568896No Hit
CTGTAATCTATGAGGATAATCAAAGACCCTCTGGGGTCCCTGATCGGTTC9470.12060959425760782No Hit
CCCAGAGGTTGCTGCTATGATAAGACTGACAGTAGTAGTCAGCCTCGTCC9410.11984543632144558No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT9240.11768032216898588No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9240.11768032216898588No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA9160.11666144492076956No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8960.11411425180022874No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC8900.1133500938640665No Hit
GTCTTATCATAGCAGCAACCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC8900.1133500938640665No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG8850.1127132955839313No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC8540.108765146247093No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG8480.10800098831093077No Hit
GTATATTACTGTGCGAGAGATCGTGGGGACATAAAGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCATCATCC8480.10800098831093077No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8460.10774626899887668No Hit
GTTCTCTGGCTCCATCGACGGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAGGAGTGAGGACGAGG8270.1053264355343629No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8170.10405283897409251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)