FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785178
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30180.38437144188960976No Hit
TCTCTGGACTGAGGAGTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT28990.3692156428223919No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27330.34807393992190305No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25170.3205642542200622No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGAGGTTGCTGCTATGATAAGACTGA25100.3196727366278729No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCCGTCGATGGAGCCA24610.3134321134825479No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAGCATTGTCAG24000.30566317446489844No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20730.264016566944056No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAGCATTGTCAGCCACTATGTG20280.25828538242283916No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18110.23064833706497126No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGAGGTTGCTGCTATGATAAGACTGACAGTAGTAGT18030.22962945981675492No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17500.22287939804732176No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17400.22160580148705136No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16700.21269062556515844No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16100.20504904620353598No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15900.2025018530829952No Hit
GACTACTACTGTCAGTCTTATCATAGCAGCAACCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT15260.19435083509726458No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15210.19371403681712934No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14540.18518093986331763No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC13890.17690256222155995No Hit
GTAGTAGTCAGCCTCGTCCTCACTCCTCAGTCCAGAGATGGTGAGGGAGG13700.17448272875704618No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13110.16696850905145075No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13090.16671378973939668No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12990.16544019317912625No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12950.1649307545550181No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12900.1642939562748829No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12580.1602184472820176No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12300.15665237691326045No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12240.15588821897709818No Hit
ATCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACGGC12150.15474198207285483No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11960.15232214860834103No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11870.15117591170409767No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11640.1482466396154757No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11610.1478645606473946No Hit
GAGTTGGAGGAGCCGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA11580.1474824816793135No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC11510.1465909640871242No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11380.14493528855877266No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG11130.14175129715809665No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10890.13869466541344763No Hit
CTACTACTGTCAGTCTTATCATAGCAGCAACCTCTGGGTGTTCGGCGGAG10730.13665691091701498No Hit
GTAATATACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGGCTGTGCTCGTGGACATGTCTGTGGT10720.13652955126098795No Hit
CAGTAGTAGTCAGCCTCGTCCTCACTCCTCAGTCCAGAGATGGTGAGGGA10590.13487387573263643No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10150.1292700508674466No Hit
GTGCAATGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTAATCTA10130.12901533155539252No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9850.12544926118663538No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9810.12493982256252723No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9760.12430302428239202No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9740.12404830497033793No Hit
CTCCTGCACCGGCAGCAGTGGCAGCATTGTCAGCCACTATGTGCAATGGTACCAGCAGCGCCCGGGCAGTGCCCC9700.12353886634622976No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9420.11997279597747264No Hit
GTCTTATCATAGCAGCAACCTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC9340.1189539187292563No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9320.11869919941720221No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9310.11857183976117518No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9180.11691616423282364No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8920.11360481317612056No Hit
CCCAGAGGTTGCTGCTATGATAAGACTGACAGTAGTAGTCAGCCTCGTCC8700.11080290074352568No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8320.10596323381449813No Hit
CTGTAATCTATGAGGATAATCAAAGACCCTCTGGGGTCCCTGATCGGTTC8310.10583587415847107No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8170.10405283897409251No Hit
GTTCTCTGGCTCCATCGACGGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAGGAGTGAGGACGAGG8060.10265188275779505No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTAATCTATGAGGAT8040.10239716344574096No Hit
CCCCAGAGGGTCTTTGATTATCCTCATAGATTACAGTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCATTGCA8010.10201508447765983No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC7990.10176036516560576No Hit
GCTTGGTCCCTCCGCCGAACACCCAGAGGTTGCTGCTATGATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCA7920.10086884757341648No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCG200.007414488651.65020840
GTATCAA23250.036.705011
GTGGTAT2900.036.200241
ATACGTA754.7881258E-836.0740811
GGTATCA11700.035.311951
CTAGAGA906.2882464E-933.8259124
TACGTAC906.3027983E-933.817312
CGTACCA808.451025E-833.81729514
TATCAAC26300.032.2969252
ATCAACG27300.030.9800593
TCAACGC27450.030.806844
CACCTAT450.00462430730.66320241
GAATACG902.3741813E-730.0617319
AATACGT902.3741813E-730.06173110
CAACGCA28200.029.8637565
TGTTCGG18350.029.76128270
AACGCAG29050.029.106376
CGCAGAG29800.028.2585338
ACGCAGA30450.027.7681477
AGAGTAC30400.027.70079811
Produced by FastQC (version 0.11.5)