FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129590
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGAGGGGCGCCGACTATTTTTATAGATGAGGAGACTGGGGGCCGCTCCTGGGACCTGCTGGTACCAGTAGA88570.7840898024947104No Hit
CCTCAGGGGTGTCTGACCGATTCTCGGGCTCCAAGTCTGGCTCCTCGGCCTCCCTGGCCATCACTAGAGTCCGGT71170.6300516116467036No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGTGTCTGGGGCCC53610.47459697766446235No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC51220.45343885834683384No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAACCACTCAGGCTGTCATCCCACGCCGCA46070.4078470949636594No Hit
ATTTATTATTGTGCGGCGTGGGATGACAGCCTGAGTGGTTTGTTCGGCGGGGGGACCAAGGTGACCGTCCTAGGT44660.3953646898432175No Hit
ATAATAAATAGCCTCATCCTCGGACCGGACTCTAGTGATGGCCAGGGAGG43800.38775130799670676No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG41400.36650466098318857No Hit
GTGTTGACTCAGCCACCCTCAGTGTCTGGGGCCCCCGGTCAGACGGTCAC41210.36482263476128507No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGTGTCT37340.33056241645198703No Hit
GACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAACCACTCAGGCTG37200.3293230287095318No Hit
CCTCTGAGGAGCTTCAGGCCAACAGGGCCACACTGGTGTGTCTCATAAGT36160.32011614833700724No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33250.29435458883311644No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCC33240.29426606113722675No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31580.27957046361954335No Hit
TCACTAGAGTCCGGTCCGAGGATGAGGCTATTTATTATTGTGCGGCGTGG31310.2771802158305226No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAGGG31270.2768261050469639No Hit
CACCAGTGTGGCCCTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31250.27664904965518466No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29850.2642551722306324No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29830.264078116838853No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28950.25628767960056303No Hit
CCCTCAGTGTCTGGGGCCCCCGGTCAGACGGTCACCATCTCATGTTCTGG27960.2475234377074868No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27770.24584141148558328No Hit
GTGTGGCCCTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC27290.24159208208287963No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG27190.24070680512398307No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCA26810.237342752680176No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAGGGCCACACTGGTGT26750.23681158650483802No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26620.2356607264582725No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC25870.22902114926654804No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25660.2271620676528652No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25550.22618826299807895No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATGGCAGCC25180.22291273825016156No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25010.22140776742003734No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24670.21839782575978897No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24340.2154764117954302No Hit
GCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC24080.21317469170229905No Hit
CTAGAGTCCGGTCCGAGGATGAGGCTATTTATTATTGTGCGGCGTGGGAT23730.210076222346161No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23020.2037907559379952No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT22560.1997184819270709No Hit
CTCCTGGGACCTGCTGGTACCAGTAGACAAAATGACTTCCAATATTGGAGCTGCTTCCAGAACATGAGATGGTGA22540.19954142653529156No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCACTGTGCAGGGTCCTGGGC22270.19715117874627078No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22270.19715117874627078No Hit
TCCTCACCCTGCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGTGTCTGGGG21940.194229764781912No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21820.1931674324312361No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21690.19201657238467054No Hit
GTCACCATCTCATGTTCTGGAAGCAGCTCCAATATTGGAAGTCATTTTGT20340.18006533343956657No Hit
GTGTCTGGGGCCCCCGGTCAGACGGTCACCATCTCATGTTCTGGAAGCAGCTCCAATATTGGAAGTCATTTTGT19710.17448808859851805No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC19660.17404545011906974No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCCTGAAGCTCCTCAGAGGA19620.17369133933551112No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19080.1689108437574695No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG18920.16749440062323498No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18640.16501562513832452No Hit
CTCTAGTGATGGCCAGGGAGGCCGAGGAGCCAGACTTGGAGCCCGAGAAT17520.15510052319868273No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17440.15439230163156542No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAACCACTCAGG17300.15315291388911023No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17140.15173647075487565No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16760.14837241831106862No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16080.1423525349905718No Hit
GCCATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16050.14208695190290282No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15780.13969670411388202No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15770.13960817641799236No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCACTG15440.13668676245363362No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15340.13580148549473703No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCCTGAAG15090.13358829309749556No Hit
ACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAACCACTCAGGCTGT14810.1311095176125851No Hit
TCCTCGGACCGGACTCTAGTGATGGCCAGGGAGGCCGAGGAGCCAGACTTGGAGCCCGAGAATCGGTCAGACACC14780.13084393452491613No Hit
GTCTGGCTCCTCGGCCTCCCTGGCCATCACTAGAGTCCGGTCCGAGGATG14720.13031276834957817No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG14550.12880779751945395No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA14480.12818810364822636No Hit
CTCCAAGTCTGGCTCCTCGGCCTCCCTGGCCATCACTAGAGTCCGGTCCG14320.1267716605139918No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAGGGCC14270.12632902203454352No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13720.12145999876061225No Hit
GGTCCCAGGAGCGGCCCCCAGTCTCCTCATCTATAAAAATAGTCGGCGCC13700.12128294336883293No Hit
GGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAG13700.12128294336883293No Hit
GTCCGAGGATGAGGCTATTTATTATTGTGCGGCGTGGGATGACAGCCTGA13650.12084030488938464No Hit
CTCATGTTCTGGAAGCAGCTCCAATATTGGAAGTCATTTTGTCTACTGGTACCAGCAGGTCCCAGGAGCGGCCCC13550.11995502793048807No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13520.11968944484281908No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG13520.11968944484281908No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT13490.1194238617551501No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13420.11880416788392248No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT13360.11827300170858453No Hit
GCTCCAATATTGGAAGTCATTTTGTCTACTGGTACCAGCAGGTCCCAGGA13330.11800741862091554No Hit
GGACTCTAGTGATGGCCAGGGAGGCCGAGGAGCCAGACTTGGAGCCCGAG13260.11738772474968794No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13200.11685655857434998No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT12980.11490894926477749No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC12820.11349250613054294No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCCTGAAG12800.11331545073876362No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12750.11287281225931534No Hit
TCCCTCTCCTCCTCACCCTGCTCACTCACTGTGCAGGGTCCTGGGCCCAG12730.11269575686753601No Hit
GGATGACAGCCTGAGTGGTTTGTTCGGCGGGGGGACCAAGGTGACCGTCC12720.11260722917164635No Hit
GAGCCAGACTTGGAGCCCGAGAATCGGTCAGACACCCCTGAGGGGCGCCG12600.11154489682097045No Hit
GTCTCTGGTGGCCCCATCACCACTTCTACTTCCTACTGGAGTTGGATCCG12490.11057109216618419No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12470.11039403677440486No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAA12350.10933170442372897No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12290.10880053824839102No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGTGTCTGGGGCCCCCGGTCAGACGGTCAC12180.10782673359360476No Hit
AGACACACCAGTGTGGCCCTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC12180.10782673359360476No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12170.1077382058977151No Hit
GACTTGGAGCCCGAGAATCGGTCAGACACCCCTGAGGGGCGCCGACTATT12060.10676440124292885No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG12050.1066758735470392No Hit
GTCTGGGGCCCCCGGTCAGACGGTCACCATCTCATGTTCTGGAAGCAGCTCCAATATTGGAAGTCATTTTGTCTA11920.10552501350047364No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCACTGTG11640.10304623801556316No Hit
CCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT11590.10260359953611488No Hit
CAATAATAAATAGCCTCATCCTCGGACCGGACTCTAGTGATGGCCAGGGA11580.10251507184022522No Hit
GTCCCAGGAGCGGCCCCCAGTCTCCTCATCTATAAAAATAGTCGGCGCCC11480.10162979488132862No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTG11450.10136421179365963No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT11440.10127568409776999No Hit
AGTCTGTGTTGACTCAGCCACCCTCAGTGTCTGGGGCCCCCGGTCAGACG11400.10092157331421135No Hit
CTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCA11380.10074451792243203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2250.052.19023541
TATACAC2500.051.9788673
AACGGTC200.00762718451.28357712
TCGTATG2100.050.938640
CTTATAC2500.050.6177251
CTAGCGC2400.049.90985528
TTATACA2550.049.6186262
CGCTCAT2300.049.1910532
TCTCGTA2100.049.18992238
ATCTCGT2100.049.12858637
ACTAGCG2450.048.8804527
TAGCGCT2500.047.92195529
GTGGTAT2500.046.5135841
CGAGCCC2500.046.49711215
ATGCCGT2400.046.21140344
GCGCTCA2600.046.12377531
GACTAGC2550.045.60962726
GCCGTCT2450.045.38031846
TCTCCGA2650.045.15135611
ACGAGAC2700.044.32502722
Produced by FastQC (version 0.11.5)