FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129590
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGAGGGGCGCCGACTATTTTTATAGATGAGGAGACTGGGGGCCGCTCCTGGGACCTGCTGGTACCAGTAGA90160.7981657061411662No Hit
CCTCAGGGGTGTCTGACCGATTCTCGGGCTCCAAGTCTGGCTCCTCGGCCTCCCTGGCCATCACTAGAGTCCGGT68690.6080967430660682No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGTGTCTGGGGCCC51860.45910463088377196No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC50070.443258173319523No Hit
ATTTATTATTGTGCGGCGTGGGATGACAGCCTGAGTGGTTTGTTCGGCGGGGGGACCAAGGTGACCGTCCTAGGT44010.38961038961038963No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG43530.38536106020768596No Hit
GTGTTGACTCAGCCACCCTCAGTGTCTGGGGCCCCCGGTCAGACGGTCAC43240.38279375702688584No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAACCACTCAGGCTGTCATCCCACGCCGCA41870.37066546269000256No Hit
ATAATAAATAGCCTCATCCTCGGACCGGACTCTAGTGATGGCCAGGGAGG39630.3508352588107189No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38790.34339893235598756No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGTGTCT35060.3103781017891447No Hit
CCTCTGAGGAGCTTCAGGCCAACAGGGCCACACTGGTGTGTCTCATAAGT33340.2951513380961234No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32810.29045937021397145No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCA32750.2899282040386335No Hit
GACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAACCACTCAGGCTG31180.27602935578395704No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30960.2740817464743845No Hit
TCACTAGAGTCCGGTCCGAGGATGAGGCTATTTATTATTGTGCGGCGTGG30790.2725767756442603No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG30490.2699209447675705No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30180.26717658619499113No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29530.26142228596216327No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCC28510.25239246098141804No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAGGG27540.24380527448012113No Hit
CCCTCAGTGTCTGGGGCCCCCGGTCAGACGGTCACCATCTCATGTTCTGG27170.2405297497322037No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27050.2394674173815278No Hit
CACCAGTGTGGCCCTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26340.233181950973362No Hit
GTGTGGCCCTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC26270.2325622571021344No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26260.23247372940624475No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25690.22742765074053417No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAGGGCCACACTGGTGT25010.22140776742003734No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATGGCAGCC24780.2193716304145752No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC24770.21928310271868554No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24620.21795518728034063No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT24570.21751254880089235No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24200.21423702405297496No Hit
GCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC24070.21308616400640942No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23990.21237794243929214No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22710.2010463973654158No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG22700.2009578696695261No Hit
CTCCTGGGACCTGCTGGTACCAGTAGACAAAATGACTTCCAATATTGGAGCTGCTTCCAGAACATGAGATGGTGA22670.20069228658185714No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22440.198656149576395No Hit
CTAGAGTCCGGTCCGAGGATGAGGCTATTTATTATTGTGCGGCGTGGGAT22220.19670854026682247No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22040.19511504174080863No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21720.1922821554723395No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21550.19077718464221533No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCACTGTGCAGGGTCCTGGGC21060.18643932754362202No Hit
TCCTCACCCTGCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGTGTCTGGGG20520.18165883196558041No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC20350.18015386113545623No Hit
GTCACCATCTCATGTTCTGGAAGCAGCTCCAATATTGGAAGTCATTTTGT20220.17900300108889064No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20090.17785214104232508No Hit
GTGTCTGGGGCCCCCGGTCAGACGGTCACCATCTCATGTTCTGGAAGCAGCTCCAATATTGGAAGTCATTTTGT18370.1626253773493037No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18230.1613859896068485No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18000.15934985260138634No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCCTGAAGCTCCTCAGAGGA17930.15873015873015872No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16830.14899211218229622No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16470.1458051151302685No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAACCACTCAGG16430.14545100434670985No Hit
CTCTAGTGATGGCCAGGGAGGCCGAGGAGCCAGACTTGGAGCCCGAGAAT16230.1436804504289167No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16040.14199842420701317No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15970.14137873033578555No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCACTGTGCAGGGTCCTGGGCCCAGT15920.14093609185633726No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15890.1406705087686683No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG15450.13677529014952328No Hit
GTCTGGCTCCTCGGCCTCCCTGGCCATCACTAGAGTCCGGTCCGAGGATG15120.13385387618516453No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCACTG15050.1332341823139369No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAA15030.1330571269221576No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14670.12987012987012986No Hit
CTCCAAGTCTGGCTCCTCGGCCTCCCTGGCCATCACTAGAGTCCGGTCCG14620.12942749139068158No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA14320.1267716605139918No Hit
GTCCGAGGATGAGGCTATTTATTATTGTGCGGCGTGGGATGACAGCCTGA14200.1257093281633159No Hit
GCCATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13860.1226993865030675No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13780.1219911649359502No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCCTGAAG13710.1213714710647226No Hit
CTCATGTTCTGGAAGCAGCTCCAATATTGGAAGTCATTTTGTCTACTGGTACCAGCAGGTCCCAGGAGCGGCCCC13580.12022061101815704No Hit
TCCCTCTCCTCCTCACCCTGCTCACTCACTGTGCAGGGTCCTGGGCCCAG13440.1189812232757018No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13430.11889269557981215No Hit
TCCTCGGACCGGACTCTAGTGATGGCCAGGGAGGCCGAGGAGCCAGACTTGGAGCCCGAGAATCGGTCAGACACC13360.11827300170858453No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13360.11827300170858453No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT13290.11765330783735692No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGTGTCTGGGGCCCCCGGTCAGACGGTCAC12970.11482042156888783No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT12930.11446631078532918No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC12880.1140236723058809No Hit
GCTCCAATATTGGAAGTCATTTTGTCTACTGGTACCAGCAGGTCCCAGGA12880.1140236723058809No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12680.11225311838808771No Hit
GGACTCTAGTGATGGCCAGGGAGGCCGAGGAGCCAGACTTGGAGCCCGAG12650.11198753530041873No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12640.11189900760452907No Hit
GGTCCCAGGAGCGGCCCCCAGTCTCCTCATCTATAAAAATAGTCGGCGCC12590.11145636912508078No Hit
GGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAG12540.1110137306456325No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCCTGAAG12510.11074814755796351No Hit
GGATGACAGCCTGAGTGGTTTGTTCGGCGGGGGGACCAAGGTGACCGTCC12490.11057109216618419No Hit
GTCTGTGTTGACTCAGCCACCCTCAGTGTCTGGGGCCCCCGGTCAGACGGTCACCATCTCATGTTCTGGAAGCAG12380.10959728751139795No Hit
GAGCCAGACTTGGAGCCCGAGAATCGGTCAGACACCCCTGAGGGGCGCCG12340.1092431767278393No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAGGGCC12320.10906612133606No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12170.1077382058977151No Hit
ACCTAGGACGGTCACCTTGGTCCCCCCGCCGAACAAACCACTCAGGCTGT12050.1066758735470392No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11920.10552501350047364No Hit
GTCTCTGGTGGCCCCATCACCACTTCTACTTCCTACTGGAGTTGGATCCG11900.10534795810869431No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11890.10525943041280465No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG11760.10410857036623906No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTG11620.10286918262378386No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11610.1027806549278942No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTGCTCACTCACTGTG11490.10171832257721829No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC11450.10136421179365963No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG11340.1003904071388734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)