FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835098
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31060.3719323959583187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27260.32642875446953534No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23350.27960790230607663No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20850.249671296063456No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19310.23123034661800174No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18280.21889646484604203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17340.20764030089881666No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGGTATTTAAATTGGTATCAGCAAAAA16720.20021602255064674No Hit
GTCCTGATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCATCAAGGTT16700.19997652970070579No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16460.1971026155014142No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT16190.19386946202721117No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16040.19207326565265392No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16020.19183377280271297No Hit
GTAACAATACGCTCACGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15970.19123504067786054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15890.1902770692780967No Hit
GGCCAAAACTGCACGTGAGCGTATTGTTACTGTGTTGACAGTAATAAGTT15250.18261329807998583No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14470.1732730769322882No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14210.17015966988305564No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14140.16932144490826226No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14040.16812398065855744No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13890.16632778428400022No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13810.16536981288423633No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13700.16405260220956103No Hit
GATCAGGACCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATTTAAAT13610.16297488438482668No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12600.15088049546280796No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG12550.15028176333795554No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12270.14692886343878203No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12150.14549190633913625No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12120.1451326670642248No Hit
GATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACACAGTAACAATACG11590.1387861065407892No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11550.1383071208409073No Hit
CCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATTTAAATACCTGCTA11320.1355529530665862No Hit
CAGTAACAATACGCTCACGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11230.13447523524185184No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11220.13435548881688136No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10990.13160132104256028No Hit
ACTTATTACTGTCAACACAGTAACAATACGCTCACGTGCAGTTTTGGCCA10910.13064334964279642No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10850.12992487109297352No Hit
GATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCATCAAGGTTCAGTGGCACTGGATCTGGGACAGATTTCA10800.12932613896812112No Hit
TATTGTTACTGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAAT10530.12609298549391806No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10480.12549425336906567No Hit
TTGTTACTGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTC10260.12285983201971505No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10260.12285983201971505No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9770.11699225719616141No Hit
CCATGGATCAGGACCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATT9720.11639352507130898No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9660.1156750465214861No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9490.1136393572969879No Hit
CACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAAC9460.11328011802207644No Hit
GAGTGAAATCTGTCCCAGATCCAGTGCCACTGAACCTTGATGGGACCCCA9220.11040620382278486No Hit
GTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGCCA9020.1080112753233752No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9000.10777178247343426No Hit
GTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACACAGTAACAAT8850.10597558609887701No Hit
CCCCTAAGGTCCTGATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCA8850.10597558609887701No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8840.10585583967390655No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8700.10417938972431978No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8620.10322141832455592No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC8610.10310167189958544No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8560.10250293977473303No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8480.10154496837496917No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8410.10070674340017578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG200.007026143452.35800647
GTGGTAT2950.043.5625951
CCCACGA1200.036.63213719
ATCTCCG1450.034.98029310
CGTATGC1102.7284841E-1134.55504241
GTATCAA23850.034.314621
CGACTGC400.002861915433.820366
CTTATAC1600.033.8183331
TCGTATG1056.4574124E-1032.84324340
GAGACCG1550.032.75684424
GTAGTTT1052.9980278E-431.80414670
CACGAGA1500.031.56944721
AGTACCG751.9497438E-631.5637745
GGGATAC1209.822543E-1130.99642418
CGGGATA1209.822543E-1130.99456817
GACCGAG1550.030.6097526
TATACAC2000.030.43653
ACGAGAC1450.030.32896622
ATGCCGT1401.2732926E-1129.74602344
TGTTAGA10400.029.54115970
Produced by FastQC (version 0.11.5)