FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835098
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29420.35229398226315956No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22480.2691899633336447No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20950.25086876031316085No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19170.22955389666841497No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18180.21769900059633718No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16720.20021602255064674No Hit
GTCCTGATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCATCAAGGTT16660.19949754400082387No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16470.19722236192638468No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16090.19267199777750635No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT15990.19147453352780153No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGGTATTTAAATTGGTATCAGCAAAAA15530.18596619797915934No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15490.1854872122792774No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15190.18189481953016293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14640.1753087661567864No Hit
GTAACAATACGCTCACGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14090.16872271278340983No Hit
GGCCAAAACTGCACGTGAGCGTATTGTTACTGTGTTGACAGTAATAAGTT13930.16680676998388214No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13890.16632778428400022No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13840.1657290521591478No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13470.16129843443523992No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13330.15962198448565318No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13250.15866401308588932No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12930.1548321274868339No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12810.15339517038718808No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG12520.1499225240630441No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12470.14932379193819167No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12320.14752759556363446No Hit
GATCAGGACCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATTTAAAT12160.14561165276410673No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12060.1444141885144019No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11810.14142052789013984No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11780.14106128861522838No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11670.13974407794055307No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA11640.13938483866564164No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10690.12800892829344582No Hit
GATTTCACTCTCACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACACAGTAACAATACG10630.12729044974362289No Hit
CCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATTTAAATACCTGCTA10560.12645222476882953No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10530.12609298549391806No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10100.12094388922018734No Hit
TTGTTACTGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTC9890.1184292142958072No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9800.11735149647107285No Hit
GATCCATGGTGCATCCAATTTGGAAAGTGGGGTCCCATCAAGGTTCAGTGGCACTGGATCTGGGACAGATTTCA9610.11507631439663368No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9610.11507631439663368No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9260.11088518952266681No Hit
CAGTAACAATACGCTCACGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9210.1102864573978144No Hit
TATTGTTACTGTGTTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAAT9160.10968772527296197No Hit
CACCATCATCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAAC9100.1089692467231391No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8970.10741254319852282No Hit
ACTTATTACTGTCAACACAGTAACAATACGCTCACGTGCAGTTTTGGCCA8930.10693355749864088No Hit
GTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGATGATGGTGAGAGTGAAATCTGTCCCAGATCCAGTGCCA8850.10597558609887701No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8710.10429913614929028No Hit
GAGTGAAATCTGTCCCAGATCCAGTGCCACTGAACCTTGATGGGACCCCA8700.10417938972431978No Hit
CCATGGATCAGGACCTTAGGGGCTTTCCCAGGTTTTTGCTGATACCAATT8530.10214370049982159No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8520.10202395407485108No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8480.10154496837496917No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC252.8671886E-453.9767653
AATGGTA2600.052.97961870
TTGGGTA400.00740922151.6551370
ATACCGT200.00804565550.594126
CCGTCAT554.1438523E-450.0898270
CCTAACG307.036711E-444.9941062
CTAGACC350.001505394338.550214
GTGGTAT4300.037.687551
TGACGTC400.00244508134.9235846
GTATCAA25250.033.8286171
CCTAGAC601.5646603E-533.7354773
GACAAAT2900.029.42001766
ATCAACG29150.029.1641163
CGGGATA1402.0008883E-1128.93518417
TATCAAC29450.028.761092
TCAACGC29650.028.6688734
AACGCAG30800.028.253866
TCAGACA3100.028.23325263
CAACGCA30450.028.1355365
CGCAGAG31750.027.4084728
Produced by FastQC (version 0.11.5)