FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945712
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC65750.6952433721894192No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGGCGAACAGTGG48910.51717647655946No Hit
GTCCTGCACTGGCACCACCCATGACATTGATTATGGCTTTGTCTCCTGGTACCAACTCCTCCCGGGGAAGGCCCC37960.40139069822525253No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34130.36089211091748863No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33110.3501065863603296No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32010.33847513830849135No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30250.3198648214255503No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27470.2904689799854501No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27420.2899402778012756No Hit
CTCCTGGACAGTCGATCACCATGTCCTGCACTGGCACCACCCATGACATT27030.2858164007647148No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26990.28539343901737524No Hit
CTCTCCCACTGTTCGCCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCC26440.27957771499145617No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25230.26678312213443417No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24950.2638223899030572No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24920.2635051685925525No Hit
GTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATGTCCTG24420.25821814675080784No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23270.2460579965147952No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTC22400.23685857851015957No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGGTCATTATTATTGCAACTCATAT22350.2363298763259851No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22240.23516673152080125No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22110.23379210584194765No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22030.23294618234726852No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATAAGGAGTTTGGGGGCCTTCCCCGGGAGGAGTTGGTACCAGGAGA21880.23136007579474513No Hit
CAGTGGGAGAGTGCTGCTGCTTATATATGAGTTGCAATAATAATGACCCT21870.23125433535791023No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21850.23104285448424045No Hit
CTCCCACTGTTCGCCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC21040.22247787910061415No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20990.22194917691643967No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20860.22057455123758607No Hit
GTCGATCACCATGTCCTGCACTGGCACCACCCATGACATTGATTATGGCT20840.22036307036391628No Hit
GTGGGAGAGTGCTGCTGCTTATATATGAGTTGCAATAATAATGACCCTCG19940.21084643104877596No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19770.20904884362258278No Hit
ACCCAGGACGGTCACCTTGGTCCCTCCGGCGAACAGTGGGAGAGTGCTGC19080.20175275348097518No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18770.19847479993909353No Hit
ACCCATGACATTGATTATGGCTTTGTCTCCTGGTACCAACTCCTCCCGGG18070.19107296936065102No Hit
GATTATGGCTTTGTCTCCTGGTACCAACTCCTCCCGGGGAAGGCCCCCAAACTCCTTATTTATGATGTCAATAAG18000.19033278630280676No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17840.1886409393134485No Hit
CCTCAGGGGTTTCCACTCGCTTCTCTGGTTCCCGGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG17590.18599742839257616No Hit
GGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATGT17220.18208503222968514No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17100.18081614698766646No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17000.1797587426193175No Hit
GACCCAGGACGGTCACCTTGGTCCCTCCGGCGAACAGTGGGAGAGTGCTG16940.17912429999830815No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16860.17827837650362902No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16800.17764393388261965No Hit
GTACCAACTCCTCCCGGGGAAGGCCCCCAAACTCCTTATTTATGATGTCAATAAGCGGCCCTCAGGGGTTTCCA16550.17500042296174734No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15970.16886747762532356No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCGGGAACCAGAGAAG15840.16749285194646996No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15780.16685840932546062No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15520.16410915796775338No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15510.1640034175309185No Hit
TATATAAGCAGCAGCACTCTCCCACTGTTCGCCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCC15490.16379193665724873No Hit
GGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGC15230.1610426852995415No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15220.1609369448627066No Hit
GCTTATATATGAGTTGCAATAATAATGACCCTCGTCCTCAGCCTGGAGCC15030.15892787656284366No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14420.1524777099159152No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14410.15237196947908033No Hit
CTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC14280.1509973438002267No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14250.15068012248972204No Hit
ACCATGTCCTGCACTGGCACCACCCATGACATTGATTATGGCTTTGTCTC13640.14422995584279358No Hit
CTTCTCTGGTTCCCGGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGC13230.13989459793256298No Hit
GTCAATAAGCGGCCCTCAGGGGTTTCCACTCGCTTCTCTGGTTCCCGGTCTGGCAACACGGCCTCCCTGACCAT13200.1395773766220583No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13200.1395773766220583No Hit
GCACTGGCACCACCCATGACATTGATTATGGCTTTGTCTCCTGGTACCAACTCCTCCCGGGGAAGGCCCCCAAA13190.1394716361852234No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGG12840.13577072089600217No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCGGGAACC12740.13471331652765323No Hit
GTGCAGGACATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG12740.13471331652765323No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12700.13429035478031368No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT12620.13344443128563452No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12580.13302146953829494No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12500.1321755460436158No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12430.13143536298577158No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12410.1312238821121018No Hit
GCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12370.1308009203647622No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCT12100.1279459285702201No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12090.1278401881333852No Hit
TTATTATTGCAACTCATATATAAGCAGCAGCACTCTCCCACTGTTCGCCG12010.12699426463870606No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11950.12635982201769672No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC11900.12583111983352227No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGT11760.12435075371783376No Hit
ACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCT11550.12213020454430101No Hit
CCTTGGTCCCTCCGGCGAACAGTGGGAGAGTGCTGCTGCTTATATATGAGTTGCAATAATAATGACCCTCGTCCT11480.12139002148645678No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC11400.12054409799177762No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC11380.12033261711810785No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11340.11990965537076828No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATGTCCTGCA11340.11990965537076828No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC11330.11980391493393337No Hit
GAGTTGGTACCAGGAGACAAAGCCATAATCAATGTCATGGGTGGTGCCAG11070.11705466357622615No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11040.11673744226572148No Hit
GTCATGGGTGGTGCCAGTGCAGGACATGGTGATCGACTGTCCAGGAGACC10980.11610299964471213No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT10960.11589151877104235No Hit
GTGCTGCTGCTTATATATGAGTTGCAATAATAATGACCCTCGTCCTCAGC10960.11589151877104235No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGGCGAACAGTGGGAGAGTG10760.1137767100343445No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10680.11293078653966536No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCGGGAACCAGAGAAGCGAGTGGAAACC10590.11197912260815134No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10560.11166190129764664No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10490.1109217182398024No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGT10370.10965283299778368No Hit
CCAGAGAAGCGAGTGGAAACCCCTGAGGGCCGCTTATTGACATCATAAAT10300.10891264993993943No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC10270.10859542862943475No Hit
CTTATTGACATCATAAATAAGGAGTTTGGGGGCCTTCCCCGGGAGGAGTT10200.10785524557159051No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG10100.10679784120324158No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10060.10637487945590202No Hit
CCTTATTTATGATGTCAATAAGCGGCCCTCAGGGGTTTCCACTCGCTTCTCTGGTTCCCGGTCTGGCAACACGGC10010.10584617727172754No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC9980.10552895596122286No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9960.10531747508755308No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACCGGGAACCAGAGAAGCGAGT9920.10489451334021352No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC9730.10288544504035056No Hit
CAGCAGCACTCTCCCACTGTTCGCCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT9670.1022510024193412No Hit
GGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG9660.1021452619825063No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9620.10172230023516673No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT9610.10161655979833183No Hit
GGTCAGGGAGGCCGTGTTGCCAGACCGGGAACCAGAGAAGCGAGTGGAAA9590.10140507892466206No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT9570.10119359805099226No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9500.10045341499314803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)