FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945712
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC64340.6803339705956993No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGGCGAACAGTGG42600.4504542609166427No Hit
GTCCTGCACTGGCACCACCCATGACATTGATTATGGCTTTGTCTCCTGGTACCAACTCCTCCCGGGGAAGGCCCC38820.4104843757930533No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35920.3798196491109344No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31440.3324479334089025No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30080.3180672339993571No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28370.2999856193005905No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27670.29258378872214796No Hit
GTGGGAGAGTGCTGCTGCTTATATATGAGTTGCAATAATAATGACCCTCG27310.2887771329960918No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27030.2858164007647148No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26560.2808466002334749No Hit
CTCCTGGACAGTCGATCACCATGTCCTGCACTGGCACCACCCATGACATT26480.28000067673879575No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26430.27947197455462125No Hit
CTCTCCCACTGTTCGCCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCC25010.2644568325240665No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24690.26107313854534997No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24560.25969851286649637No Hit
GTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATGTCCTG24520.2592755511191568No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGGTCATTATTATTGCAACTCATAT23150.24478911127277647No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22500.2379159828785085No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22120.23389784627878257No Hit
CAGTGGGAGAGTGCTGCTGCTTATATATGAGTTGCAATAATAATGACCCT21690.2293510074948822No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21450.22681323701084474No Hit
GTCGATCACCATGTCCTGCACTGGCACCACCCATGACATTGATTATGGCT21280.22501564958465153No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21130.2234295430321282No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATAAGGAGTTTGGGGGCCTTCCCCGGGAGGAGTTGGTACCAGGAGA21000.22205491735327454No Hit
CTCCCACTGTTCGCCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC20650.21835400206405334No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20620.21803678075354863No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20460.21634493376419037No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTC20220.21380716328015295No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19060.2015412726073054No Hit
ACCCATGACATTGATTATGGCTTTGTCTCCTGGTACCAACTCCTCCCGGG18190.19234185460266973No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17910.18938112237129273No Hit
GATTATGGCTTTGTCTCCTGGTACCAACTCCTCCCGGGGAAGGCCCCCAAACTCCTTATTTATGATGTCAATAAG17670.18684335188725532No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17640.18652613057675063No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17620.18631464970308087No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17160.18145058960867577No Hit
GGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATGT17130.18113336829817112No Hit
CCTCAGGGGTTTCCACTCGCTTCTCTGGTTCCCGGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG16680.17637504864060094No Hit
CTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC16660.17616356776693115No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16650.17605782733009626No Hit
GGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGC16420.17362579728289373No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGG16190.1711937672356912No Hit
ACCCAGGACGGTCACCTTGGTCCCTCCGGCGAACAGTGGGAGAGTGCTGC16050.16971340112000272No Hit
GTACCAACTCCTCCCGGGGAAGGCCCCCAAACTCCTTATTTATGATGTCAATAAGCGGCCCTCAGGGGTTTCCA16040.16960766068316782No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15820.1672813710728002No Hit
TATATAAGCAGCAGCACTCTCCCACTGTTCGCCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCC15620.16516656233610233No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15190.16061972355220194No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14850.15702454869981558No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14720.15564992302096198No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14600.15438103777894327No Hit
GCTTATATATGAGTTGCAATAATAATGACCCTCGTCCTCAGCCTGGAGCC14580.15416955690527348No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14400.15226622904224543No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCGGGAACCAGAGAAG14280.1509973438002267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14020.1482480924425195No Hit
CTTCTCTGGTTCCCGGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGC13900.14697920720050078No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13670.14454717715329826No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13480.1425381088534353No Hit
ACCATGTCCTGCACTGGCACCACCCATGACATTGATTATGGCTTTGTCTC13000.13746256788536043No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12960.13703960613802088No Hit
GTCAATAAGCGGCCCTCAGGGGTTTCCACTCGCTTCTCTGGTTCCCGGTCTGGCAACACGGCCTCCCTGACCAT12890.13629942308017665No Hit
GACCCAGGACGGTCACCTTGGTCCCTCCGGCGAACAGTGGGAGAGTGCTG12860.13598220176967193No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12790.1352420187118277No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12720.13450183565398344No Hit
GTGCAGGACATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG12630.1335501717224694No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12550.13270424822779028No Hit
GCACTGGCACCACCCATGACATTGATTATGGCTTTGTCTCCTGGTACCAACTCCTCCCGGGGAAGGCCCCCAAA12470.13185832473311115No Hit
TTATTATTGCAACTCATATATAAGCAGCAGCACTCTCCCACTGTTCGCCG12430.13143536298577158No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12320.13027221818058776No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12180.12879185206489926No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12150.12847463075439455No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATGTCCTGCA11930.12614834114402693No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGT11760.12435075371783376No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11730.12403353240732908No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11730.12403353240732908No Hit
GCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11670.12339908978631974No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11660.12329334934948484No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT11530.12191872367063124No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11520.12181298323379634No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC11410.12064983842861252No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11390.12043835755494274No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCGGGAACC11360.12012113624443804No Hit
GTGCTGCTGCTTATATATGAGTTGCAATAATAATGACCCTCGTCCTCAGC11330.11980391493393337No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC11270.11916947231292402No Hit
CCTTGGTCCCTCCGGCGAACAGTGGGAGAGTGCTGCTGCTTATATATGAGTTGCAATAATAATGACCCTCGTCCT11180.11821780838140998No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC11090.11726614444989596No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11050.11684318270255639No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGT10960.11589151877104235No Hit
ACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCT10830.11451689309218874No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGGCTCAGCCT10820.11441115265535386No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10800.11419967178168407No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10700.11314226741333513No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10650.11261356522916068No Hit
GAGTTGGTACCAGGAGACAAAGCCATAATCAATGTCATGGGTGGTGCCAG10630.1124020843554909No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10590.11197912260815134No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10350.1094413521241139No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC10070.10648061989273691No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT10010.10584617727172754No Hit
CAGCAGCACTCTCCCACTGTTCGCCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT9910.10478877290337862No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGGCGAACAGTGGGAGAGTG9770.10330840678769013No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9720.10277970460351565No Hit
CCTTATTTATGATGTCAATAAGCGGCCCTCAGGGGTTTCCACTCGCTTCTCTGGTTCCCGGTCTGGCAACACGGC9700.10256822372984586No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGC9680.10235674285617609No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT9640.10193378110883652No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC9540.10087637674048759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGA200.00704367652.3256249
AATCGGG352.0629619E-550.09865655
TGAATCG352.0862222E-550.00378853
CGGTAAC306.549387E-445.6549918
GTACTAG306.5579324E-445.642911
GGTATCA6150.045.086291
GAATCGG404.5686356E-543.7912254
TGGTATC1800.043.7110522
TCACTAG754.7829613E-543.44743370
GTATCAA21200.042.467281
GTGGTAT1850.040.7085381
ATCGGGT458.975855E-539.05241456
TATCAAC23350.038.2375072
ATCAACG24600.036.2945443
TCAACGC25150.035.364814
CAACGCA25450.034.9497835
AACGCAG26050.034.2797476
TCGGTAA400.002695825634.23580617
GTAGTTT1151.4812951E-534.0023470
AGAGTAC27600.032.50264411
Produced by FastQC (version 0.11.5)