FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224892
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60870.49694177119288885No Hit
GAGTTTACACTGAAAATCAGCAGAGTGGAGGCTGACGATGTTGGGGTTTA54580.44559030510445No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47020.383870577977487No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42500.34696936546242446No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39070.31896689667333933No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38760.3164360613017311No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAAGTCTAATCAGAGCCTCCTA38210.3119458695133938No Hit
GTGTAAACTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG36690.2995366122074436No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35350.28859687221404007No Hit
GTAGTAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTTTCAGTGTAAACTCTGTGCCTGATCCACTGCCA34130.27863681042900107No Hit
CTCTACAACTCGCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCGAA33700.27512629684902834No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33620.274473178043452No Hit
GGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG33050.2698197065537207No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31810.25969636506728755No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31440.25667569059149703No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28800.2351227700074782No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28700.2343063715005078No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27880.22761190374335044No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27480.22434630971546876No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26760.21846824046528185No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26690.21789676151040255No Hit
GTATTACTGTGCGAGAGGCATCATTGTGGATATTCCATCTGCTCAACCGG26140.2134065697220653No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24850.202875028982147No Hit
GATCAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24650.20124223196820618No Hit
GTTGTAGAGTTTGCATGCAGTAGTAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTTTCAGTGTAAACT24240.19789499808962752No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23950.19552744241941328No Hit
ATGTTGGGGTTTACTACTGCATGCAAACTCTACAACTCGCGCTCACTTTC23730.1937313657040784No Hit
CAGTAATACCGAGCCGTGTCCGCGGCGGTGACAGACGTCAATTTCAGGGAGAACTGGTTCCTTGACTTGTCGA23530.19209856869013758No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23390.19095561078037898No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23290.1901392122734086No Hit
CCTCAGGTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG23250.18981265287062044No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT22870.18671033854413288No Hit
GTTTACTACTGCATGCAAACTCTACAACTCGCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCGAACGAA22670.18507754153019204No Hit
GTGCAGCCACAGTTCGTTCGATCTCCACCCTGGTCCCTCCGCCGAAAGTG22270.18181194750231042No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATCTCCACCCTGGTCC22240.18156702795021928No Hit
GGCACAGAGTTTACACTGAAAATCAGCAGAGTGGAGGCTGACGATGTTGGGGTTTACTACTGCATGCAAACTCTA21880.17862799332512583No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21810.17805651437024653No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAAGTCTA20660.1686679315400868No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT20630.16842301198799567No Hit
GGGTGGAGATCGAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20250.16532069766150811No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTCCGGTGGGGATATTGTCCTGA20200.1649124984080229No Hit
AGCCAGGGCAGTCTCCACGACTCCTGATCTACTTGGGTTTTAATCGGGCC19810.1617285442308383No Hit
ATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGCGCGAGTTGTAGAGTTTG19210.15683015318901586No Hit
GCTAATGCTCTGGGTCTCTGGTTCCGGTGGGGATATTGTCCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC18810.1535645591611342No Hit
ATATTGTCCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAG18790.15340127945974014No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTCCGGTGGGGATATTGTCCTGACTCAGTCTCCACTCTCC18690.1525848809527697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18270.14915600722349398No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18210.14866616811931174No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18160.14825796886582654No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17990.14687009140397683No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17680.14433925603236855No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGAGTTTACACTGAA17620.14384941692818634No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGGTCACCTTCTCACA17350.14164514095936623No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17320.1414002214072751No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17180.14025726349751652No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16700.13633855066405856No Hit
GTCCTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGC16570.13527723260499702No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTCCGGTGGGGATATTGTCCTGACTCAGTCTCCACT16230.1325014776812976No Hit
TTACTACTGCATGCAAACTCTACAACTCGCGCTCACTTTCGGCGGAGGGA16130.1316850791743272No Hit
CAGTAGTAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTTTCAGTGTAAACTCTGTGCCTGATCCACTGCCA15970.13037884156317456No Hit
GTCAGGGACCCCGGAGGCCCGATTAAAACCCAAGTAGATCAGGAGTCGTGGAGACTGCCCTGGCTTCTGCACGTA15660.12784800619156628No Hit
GTTCAGTGGCAGTGGATCAGGCACAGAGTTTACACTGAAAATCAGCAGAGTGGAGGCTGACGATGTTGGGGTTTA15630.12760308663947514No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCAGGACAATATCC15480.12637848887901953No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15470.1262968490283225No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15200.12409257305950239No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15160.12376601365671422No Hit
CTCCACGACTCCTGATCTACTTGGGTTTTAATCGGGCCTCCGGGGTCCCT15110.12335781440322902No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15090.12319453470183493No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14950.12205157679207636No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14780.12066369933022666No Hit
CCCCAACATCGTCAGCCTCCACTCTGCTGATTTTCAGTGTAAACTCTGTGCCTGATCCACTGCCACTGAAC14750.12041877977813553No Hit
CTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCC14710.12009222037534738No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14700.12001058052465033No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14610.11927582186837696No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14610.11927582186837696No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14550.11878598276419472No Hit
GAGTGGAGGCTGACGATGTTGGGGTTTACTACTGCATGCAAACTCTACAACTCGCGCTCACTTTCGGCGGAGGGA14410.11764302485443616No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14250.1163367872432835No Hit
GTCTCTGGTGACTCCATCACCAGTACGGCCTGGTGGACTTGGGTCCGCCA14200.1159285879897983No Hit
AGTCTAATCAGAGCCTCCTACGTAGTAATGGAAAGAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTC14170.11568366843770715No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14090.11503054963213084No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13940.11380595187167522No Hit
GACCAGGGTGGAGATCGAACGAACTGTGGCTGCACCATCTGTCTTCATCT13830.11290791351400775No Hit
GTAATGGAAAGAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCT13800.11266299396191665No Hit
GCCCTGGCTTCTGCACGTACCAATCCAAATAGTTCTTTCCATTACTACGT13480.11005051873961133No Hit
GCCACAGTTCGTTCGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGCGC13390.10931576008333796No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13340.10890756082985276No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13320.10874428112845867No Hit
GAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACGACTCCTGATCTACTTGGGTTTTAATCGGGC13310.10866264127776164No Hit
GTAATACCGAGCCGTGTCCGCGGCGGTGACAGACGTCAATTTCAGGGAGAACTGGTTCCTTGACTTGTCGACTGA13280.1084177217256705No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12990.10605016605545632No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12880.10515212769778887No Hit
CTCCTACGTAGTAATGGAAAGAACTATTTGGATTGGTACGTGCAGAAGCCAGGGCAGTCTCCACGACTCCTGAT12880.10515212769778887No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12860.10498884799639478No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12790.1044173690415155No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12650.10327441113175692No Hit
GTCGTGGAGACTGCCCTGGCTTCTGCACGTACCAATCCAAATAGTTCTTT12410.10131505471502793No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12390.10115177501363387No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12340.10074357576014864No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12310.10049865620805752No Hit
CTCCTCAGGTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGTTC12290.10033537650666344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)