FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847335
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31390.3704556049260328No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28230.3331622085715803No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24190.2854833094348752No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21610.25503490355054376No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTCCTGATCTATTTGGGTT21130.24937008385113327No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG20740.2447674178453622No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20520.24217104214979907No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC19150.22600270259106495No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17940.2117226362654676No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17910.21136858503425449No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17550.20711997025969658No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17410.20546773118070186No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17310.20428756040999133No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16730.1974425699398703No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16690.1969705016315861No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15890.1875291354659019No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15370.1813922474582072No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGCGTCCCTTGGGCGAAGGTGGGAGGAGTTTG15370.1813922474582072No Hit
GTAATAAATCCCAACGTCCTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAATCTGTGCCTGATCCACTGCCA14990.17690759852950722No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14840.17513734237344145No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGCGTCCCTTGGGCGAAGGTG14340.16923648851988884No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14220.1678202835950362No Hit
CTGCAAACTCCTCCCACCTTCGCCCAAGGGACGCGACTGGAGATTAAACG14100.16640407867018356No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTAAA14030.1655779591306862No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT13930.1643977883599757No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13410.15826090035228096No Hit
AGCCAGGGCAGTCTCCACAGTTCCTGATCTATTTGGGTTCTAATCGGGCC13280.15672667835035728No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13060.15413030265479416No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT12950.1528321148070126No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA12870.15188797819044414No Hit
GTTCCTGATCTATTTGGGTTCTAATCGGGCCCCCGGGGTCCCTGACAGGT12770.15070780741973364No Hit
GAGTTTGCAGAGCTTGCATGCAGTAATAAATCCCAACGTCCTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAAT12480.1472853121846731No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12300.1451610047973942No Hit
GTCAGGGACCCCGGGGGCCCGATTAGAACCCAAATAGATCAGGAACTGTGGAGACTGCCCTGGCTTCTGCAGGTA12290.14504298772032312No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11960.1411484241769784No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC11890.14032230463748102No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11680.13784394601898894No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11580.1366637752482784No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTAAAAATCAGCAGAG11550.13630972401706526No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC11370.13418541662978634No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11090.13088093847179685No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10830.1278124944679495No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10710.1263962895430969No Hit
GTCTCCACAGTTCCTGATCTATTTGGGTTCTAATCGGGCCCCCGGGGTCC10690.12616025538895478No Hit
GAGTGGAGGCTGAGGACGTTGGGATTTATTACTGCATGCAAGCTCTGCAAACTCCTCCCACCTTCGCCCAAGGGA10680.12604223831188371No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT10410.12285577723096532No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC10410.12285577723096532No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC10310.1216756064602548No Hit
GTGGAGGCTGAGGACGTTGGGATTTATTACTGCATGCAAGCTCTGCAAACTCCTCCCACCTTCGCCCAAGGGACG10200.12037741861247322No Hit
GATTTTACACTAAAAATCAGCAGAGTGGAGGCTGAGGACGTTGGGATTTATTACTGCATGCAAGCTCTGCAAACT10200.12037741861247322No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10150.11978733322711796No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10080.1189612136876206No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10010.11813509414812322No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT9990.11789905999398113No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9610.11341441106528115No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9590.11317837691113904No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTCCTGATCTATTTGGGTTCTAAT9580.113060359834068No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9510.11223424029457062No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9410.1110540695238601No Hit
CAGTAATAAATCCCAACGTCCTCAGCCTCCACTCTGCTGATTTTTAGTGTAAAATCTGTGCCTGATCCACTGCCA9390.11081803536971799No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9180.1083396767512259No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9100.10739554013465748No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9090.10727752305758644No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCT8980.10597933520980485No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC8950.1056252839785917No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA8900.10503519859323643No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8870.10468114736202327No Hit
GGCACAGATTTTACACTAAAAATCAGCAGAGTGGAGGCTGAGGACGTTGGGATTTATTACTGCATGCAAGCTCTG8780.10361899366838383No Hit
CTCCTCCCACCTTCGCCCAAGGGACGCGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC8740.1031469253600996No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8680.1024388228976733No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8670.10232080582060223No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8610.10161270335817593No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8590.10137666920403382No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGC8580.10125865212696278No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTCCTGAT8560.10102261797282067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)