FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329797
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG47530.35742297508567095No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34580.2600396902685147No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA31770.2389086454549078No Hit
GTATTACTGTCAGCAATATGTTAGCTCACCGTGGACGTTCGGCCAAGGGA29100.21883039290959447No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27700.20830247022665865No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26330.19800014588692863No Hit
GTATAATAACCACTCGGAGGAACAGTAATACACGGCTGTATCCTCGGTTTTCAGGCTGTTCATTTGCAGATACAG25890.19469137018657737No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25690.19318738123187223No Hit
CAGTAATACACTACAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25210.18957780774057995No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC25120.18890101271096263No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24260.18243386020573066No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23940.18002747787820247No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23270.17498911487994034No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22340.16799556624056153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20470.1539332695140687No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20440.15370767117086293No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20280.15250448000709882No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19620.14754131645657195No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19610.1474661170088367No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19420.14603732750186682No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT19120.14378134406980916No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18950.1425029534583098No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18550.13949497554889959No Hit
CCCTTAGACTCTCTTGTGCAGCCTCTGGATTCACTTTCAGTAACACCTGG17770.1336294186255496No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC17720.13325342138687332No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT17640.1326518258049913No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC17630.13257662635725603No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17500.1315990335366977No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC17040.12813985894087596No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCACCTACTTAGCCTGGTACC16890.1270118672248471No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16610.12490628268825993No Hit
GTGTATTACTGTCAGCAATATGTTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA16400.12332709428581956No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT16220.12197350422658497No Hit
CTCTTGTGCAGCCTCTGGATTCACTTTCAGTAACACCTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG16200.12182310533111446No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16180.12167270643564393No Hit
GTAATACACGGCTGTATCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA15980.12016871748093882No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15820.11896552631717472No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15670.11783753460114588No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15650.11768713570567539No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGGTGCTGGTAACACTCTGA15630.11753673681020486No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15630.11753673681020486No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15480.11640874509417604No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15360.11550635172135296No Hit
ATGTTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA15260.1147543572440004No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAG15120.11370156497570681No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15050.11317516884156002No Hit
GTAATACACTACAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15010.11287437105061901No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC14870.11182157878232542No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCA14780.11114478375270812No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC14760.11099438485723762No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14590.10971599424573826No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14530.10926479755932672No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14420.10843760363423892No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA14360.10798640694782737No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA14330.1077608086046216No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14210.10685841523179852No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA14180.10663281688859276No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14150.106407218545387No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14110.10610642075444597No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13890.10445203290427034No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13790.10370003842691779No Hit
GGGCTGGAGTGGGTTGGCCGTATTAAAAGCAAAACTGATGGTGGGACAAC13520.1016696533380659No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATCCAGGTGTTAC13440.10106805775618383No Hit
GTTATTATACCGCATGGGATTCTACGGTATGGACGTCTGGGGCCAAGGGA13300.10001526548789026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGTC200.00598823354.5190262
CGTTCCG200.007394922451.68675640
TTCCGAT200.00792960850.7820858
ATATAGG200.007961513550.730533
GGTATCA5700.043.9186861
GTATCAA15050.039.336351
GTATAAG702.6033376E-838.662011
TACCCTT350.001486362238.6518335
TATCAAC17650.033.1509442
TCTACAC1450.032.6541373
TAATCGT450.004085539431.45094953
GTACTGT652.6551841E-531.2270071
ATCAACG18800.031.1219223
TCAACGC19200.030.4724034
CAACGCA19850.029.4756765
AACGCAG20250.029.0702926
AATGGTA5650.028.76478670
GCATCGT605.7313463E-428.47919535
TTAATCG500.0069160228.24030952
GCTCGGG5450.027.33534270
Produced by FastQC (version 0.11.5)