FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788199
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT24850.3152757108293718No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23900.30322291705521065No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21220.26922135146073517No Hit
GTAATAAAGAGCCGTGTCGGCGGCACTCACAGAGGTCAGCCTCAGGGAGA20920.2654152060583685No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG19600.2486681662879552No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19360.24562324996606189No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18770.23813783067474079No Hit
CTTTATTACTGTGTGAGACATGATTGTGGTGGTGCCTGCCATTATACTTACTACTACTACTACTACATGGACGT18310.23230174105777854No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18180.2306524113834197No Hit
GTCTCTGGTGGCTCCATCGGCAGTAGTAGTTACTTCTGGGGCTGGATCCG17170.21783838852878523No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16800.21314414253253305No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16250.20616620929486082No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACATT15870.20134509178519636No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGATCAGACTGTTCCTGCTTGAATATGAGCTGCAATAATAAT15630.19830017546330303No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCCGATTATTATTGCAGCTCATAT15280.19385967249387528No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACATTGGTGGTTATGACTTTGTCTCCTGGTACCAACAACA15190.19271782887316527No Hit
CCATTATACTTACTACTACTACTACTACATGGACGTCTGGGGCAAGGGGACCACGGTCACCGTCTCCTCAGCATC15110.19170285676586749No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15090.19144911373904305No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14800.18776983985008863No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14600.18523240958184417No Hit
GTATAATGGCAGGCACCACCACAATCATGTCTCACACAGTAATAAAGAGC14310.18155313569288975No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14170.1797769345051186No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGATCAGACTGTTCCTGCTTGAATATGAGCTG14100.17888883391123309No Hit
CTCCTGCACTGGAACCAACAGTGACATTGGTGGTTATGACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC13850.17571704607592753No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGATCAGACTGTTC13490.17114967159308755No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13290.1686122413248431No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGATCAGACTGTTCCTGC13210.1675972692175453No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13040.16544045348953754No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12920.16391799532859086No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12730.16150743657375866No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12570.15947749235916311No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12530.15897000630551422No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCGGCAGTAGTAGTTACTTCTGGG12340.156559447550682No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11980.15199207306784202No Hit
CTATTATAGTGGGACCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCGGAGACACGTCGAAGAA11410.14476039680334535No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11200.14209609502168868No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCGGAGACACGTCGAAGAACCAAATCTCCCTGAGG11190.14196922350827645No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11060.14031989383391757No Hit
CAGTAATAAAGAGCCGTGTCGGCGGCACTCACAGAGGTCAGCCTCAGGGA11020.1398124077802687No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10590.13435693270354315No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10510.13334196059624537No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10380.1316926309218865No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10340.1311851448682376No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9830.12471469768421427No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9790.12420721163056538No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9780.12408034011715315No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCGGAGACACGTCGAAGAACCAAATCTCCCTGAGGCTGA9580.12154290984890873No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9570.1214160383354965No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9550.12116229530867205No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9460.12002045168796205No Hit
TTATTACTGTGTGAGACATGATTGTGGTGGTGCCTGCCATTATACTTACT9420.11951296563431316No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9420.11951296563431316No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9410.11938609412090093No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9330.11837112201360317No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9050.11481871963806094No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8940.1134231329905265No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8880.11266190391005318No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8870.11253503239664095No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8870.11253503239664095No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8870.11253503239664095No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8820.11190067482957984No Hit
GTTCCTGCTTGAATATGAGCTGCAATAATAATCGGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGC8780.11139318877593095No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8760.11113944574910652No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8560.10860201548086205No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8550.10847514396744985No Hit
AGCAGGAACAGTCTGATCTTCGGAACTGGGACCAAGGTCACCGTCCTAGG8470.10746017186015207No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8330.10568397067238096No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8300.1053033561321443No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC8230.10441525553825874No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8080.1025121828370754No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8040.10200469678342651No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT7980.10124346770295319No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7930.10060911013589208No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG7890.10010162408224318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC150.002435134768.4221513
ATGTAAC200.007593592651.3394171
CAGCCGT200.00760121851.3263852
TATCTCG552.6593625E-950.04733336
GCGGCAT400.00898591349.1942970
GGTATCA5500.049.161381
ATGCCGT751.6370905E-1146.2039744
GAGCCTA803.6379788E-1142.85356531
GTACGTA409.342001E-442.47617369
GATCTAG350.00140136939.1157461
CGTATGC901.2187229E-1038.3775341
TCGTATG901.2187229E-1038.3406940
ACGAGAC901.3460522E-1038.01230622
ATCTCGT754.0954546E-836.72012337
GAGACCG952.401066E-1036.0162324
GTATCAA13550.035.8681261
TCTCGTA807.1575414E-834.46251738
GACCGGA1004.1472958E-1034.22193526
CTCGATC400.00270512134.2110811
ACTGATG601.4221836E-534.2089048
Produced by FastQC (version 0.11.5)