FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788199
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23220.29459565414317956No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22420.2844459330702018No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT21580.2737887259435752No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC20410.2589447588743452No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG18810.23864531672838965No Hit
GTAATAAAGAGCCGTGTCGGCGGCACTCACAGAGGTCAGCCTCAGGGAGA18640.23648850100038188No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17510.22215201998480077No Hit
GTCTCTGGTGGCTCCATCGGCAGTAGTAGTTACTTCTGGGGCTGGATCCG17320.21974146122996857No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG16840.21365162858618192No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16640.21111419831793746No Hit
CTTTATTACTGTGTGAGACATGATTGTGGTGGTGCCTGCCATTATACTTACTACTACTACTACTACATGGACGT16230.20591246626803636No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15580.19766581789624196No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15510.19677771730235638No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACATT15280.19385967249387528No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCCGATTATTATTGCAGCTCATAT15040.19081475617198196No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGATCAGACTGTTCCTGCTTGAATATGAGCTGCAATAATAAT14580.18497866655501974No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGATCAGACTGTTCCTGCTTGAATATGAGCTG14040.17812760483075976No Hit
CCATTATACTTACTACTACTACTACTACATGGACGTCTGGGGCAAGGGGACCACGGTCACCGTCTCCTCAGCATC13780.17482894548204198No Hit
CTCCTGCACTGGAACCAACAGTGACATTGGTGGTTATGACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC13470.1708959285662631No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13420.17026157099920197No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13340.16924659889190422No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACATTGGTGGTTATGACTTTGTCTCCTGGTACCAACAACA13200.16747039770413308No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13160.16696291165048419No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13110.1663285540834231No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13080.16594793954318643No Hit
GTATAATGGCAGGCACCACCACAATCATGTCTCACACAGTAATAAAGAGC12920.16391799532859086No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGATCAGACTGTTC12740.16163430808717089No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12360.15681319057750645No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCGGCAGTAGTAGTTACTTCTGGG12270.15567134695679646No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12220.15503698938973534No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12190.15465637484949865No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11940.1514845870141931No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11880.15072335793371977No Hit
CTATTATAGTGGGACCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCGGAGACACGTCGAAGAA11610.14729782707158978No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGATCAGACTGTTCCTGC11580.14691721253135312No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11360.14412603923628423No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11170.14171548048145202No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10890.1381630781059098No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10760.13651374843155092No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCGGAGACACGTCGAAGAACCAAATCTCCCTGAGG10750.1363868769181387No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10300.1306776588145887No Hit
CAGTAATAAAGAGCCGTGTCGGCGGCACTCACAGAGGTCAGCCTCAGGGA9950.12623715584516093No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG9890.1254759267646876No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9850.12496844071103871No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9580.12154290984890873No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9490.12040106622819872No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9430.11963983714772539No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9160.1162143062855954No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9040.11469184812464873No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC8960.11367687601735095No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8920.11316938996370204No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8900.11291564693687761No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8810.11177380331616761No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8510.10796765791380096No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8460.10733330034673985No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8420.10682581429309096No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8360.10606458521261763No Hit
GTTCCTGCTTGAATATGAGCTGCAATAATAATCGGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGC8360.10606458521261763No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8310.10543022764555651No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG8270.10492274159190763No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8240.10454212705167097No Hit
TTATTACTGTGTGAGACATGATTGTGGTGGTGCCTGCCATTATACTTACT8190.10390776948460984No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCTTATCCGGAGACACGTCGAAGAACCAAATCTCCCTGAGGCTGA8120.10301966889072431No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8060.10225843981025097No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8040.10200469678342651No Hit
CCCTGAGGCTGACCTCTGTGAGTGCCGCCGACACGGCTCTTTATTACTGTGTGAGACATGATTGTGGTGGTGC8020.10175095375660208No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7940.10073598164930431No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7920.10048223862247985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCTT451.5521699E-461.1050970
GGTATCA6350.045.2166981
GTGGTAT1850.038.8008161
GTATCAA15750.037.5455781
TGGTATC1950.036.7806552
TATCAAC18700.031.4138832
GGATATT2750.031.0742451
CGGACCG450.004659983330.6151238
ATCAACG19150.030.4915433
TCAACGC19550.030.0404344
AACGCAG19850.029.7584386
CAACGCA19950.029.6092745
CTGGAGT11700.028.78989870
ACGCAGA21000.027.9662157
AGAGTAC21250.027.79611811
CGCAGAG21200.027.7023838
CAGAGTA21350.027.66592610
GCAGAGT21400.027.441749
GTATTAC500.00809126827.3453351
GGCGGAC658.8121445E-426.45660236
Produced by FastQC (version 0.11.5)