FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291181
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56840.44021713454581507No Hit
GTATTACTGTCAAGAATTTGGTGGCTCACCTCCGTTCACTTTTGGCCAGGGGACCAGACTGGAGATCAAACGAA53570.4148914830686016No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50530.3913471465270941No Hit
GTGTATTACTGTCAAGAATTTGGTGGCTCACCTCCGTTCACTTTTGGCCA46000.3562629871412296No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA44380.3437163341158211No Hit
CTCCTGCAGGGTCAGTCTGAGTGTCTCCAACAACTACTTAGCCTGGTACC42970.33279609907518776No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTTTGGCGATGGTGAGAGTGA42390.3283040874981896No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGAGAAGAAGCCGCCCTCTCCTGCAGGGTCAGTCTGAGTGTCTCCAA40920.3169191616047634No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTTTGGCGATGGTGAGAGTGAAGTCTGCCCCAGACCCACTGCCA38450.29778938816478867No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37550.29081902537289506No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37020.2867142561732244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35720.27664595436271133No Hit
GTCTGAGTGTCTCCAACAACTACTTAGCCTGGTACCAGCAAAGGCCTGGC35060.27153435498198936No Hit
GTGTTGACACAGTCTCCAGACACCCTGTCTTTGTCTCCAGGAGAAGAAGC31970.24760277606315462No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30940.23962558309020965No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29320.22707893006480112No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29280.2267691361629392No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28480.22057325812570042No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26970.20887853833041226No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26240.20322479962143186No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26050.20175327858758763No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26010.2014434846857257No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25910.20066899993107087No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23880.18494695941157746No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23600.1827784020985439No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCTGGTCCCCTGGCCAAAAGTG23420.18138432954016515No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23320.18060984478551032No Hit
CACTCAGACTGACCCTGCAGGAGAGGGCGGCTTCTTCTCCTGGAGACAAA21910.16968960974487698No Hit
CATTCATAGTGTTTCGACCAGGGCCGCTGGCGTCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGGCAGACTTCA21670.16783084633370535No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCTGGTCC21630.1675210524318434No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT21600.16728870700544696No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20920.162022210673794No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20710.16039579268901882No Hit
GCCCCAGACCCACTGCCACGGAACCTGTCTGGGACGCCAGCGGCCCTGGT20160.15613612653841716No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT20160.15613612653841716No Hit
CTGCAGGAGAGGGCGGCTTCTTCTCCTGGAGACAAAGACAGGGTGTCTGG19780.15319308447072874No Hit
GAGTGAAGTCTGCCCCAGACCCACTGCCACGGAACCTGTCTGGGACGCCA19670.1523411512406084No Hit
GGCCTGGCCAGGCTCCCAGGCTCCTCATTCATAGTGTTTCGACCAGGGCC19620.15195390886328097No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT19610.1518764603878155No Hit
CTTTTGGCCAGGGGACCAGACTGGAGATCAAACGAACTGTGGCTGCACCA19290.14939810917291999No Hit
CTATGAATGAGGAGCCTGGGAGCCTGGCCAGGCCTTTGCTGGTACCAGGCTAAGTAGTTGTTGGAGACACTCAGA19210.1487785213691961No Hit
GTCTGGGGCAGACTTCACTCTCACCATCGCCAAACTGGAGCCTGAAGATT18790.14552568539964575No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18670.14459630369405993No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18400.14250519485649185No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18310.14180815857730247No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGACAAATGGTGTTGACACAGTC18040.1397170497397344No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17640.136619110721115No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17380.1346054503590124No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17350.13437310493261595No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17340.13429565645715047No Hit
GACTTCACTCTCACCATCGCCAAACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAAGAATTTGGTGGCTCA17270.13375351712889208No Hit
GGCCAAAAGTGAACGGAGGTGAGCCACCAAATTCTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTTTGG17130.13266923847237527No Hit
GTCTGGGACGCCAGCGGCCCTGGTCGAAACACTATGAATGAGGAGCCTGGGAGCCTGGCCAGGCCTTTGCTGGTA17110.1325143415214443No Hit
GAGGTGAGCCACCAAATTCTTGACAGTAATACACTGCAAAATCTTCAGGC16830.13034578420841075No Hit
GTCTTTGTCTCCAGGAGAAGAAGCCGCCCTCTCCTGCAGGGTCAGTCTGA16620.12871936622363556No Hit
GTCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGGCAGACTTCACTCTCAC16530.1280223299444462No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACCGGACAAATGGTGTTGACACAGTCTC16470.1275576390916533No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16390.12693805128792943No Hit
CTCCAGACACCCTGTCTTTGTCTCCAGGAGAAGAAGCCGCCCTCTCCTGC15900.12314307599012068No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15570.12058727629975967No Hit
CTCCCAGGCTCCTCATTCATAGTGTTTCGACCAGGGCCGCTGGCGTCCCA15470.11981279154510482No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14640.1133845680814696No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14410.11160325314576346No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14290.11067387144017764No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14150.10958959278366084No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14140.10951214430819536No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14130.10943469583272987No Hit
GGACCAGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14100.10920235040633341No Hit
GCAAAGGCCTGGCCAGGCTCCCAGGCTCCTCATTCATAGTGTTTCGACCAGGGCCGCTGGCGTCCCAGACAGGTT14070.10897000497993697No Hit
GCCTGGGAGCCTGGCCAGGCCTTTGCTGGTACCAGGCTAAGTAGTTGTTG13880.10749848394609277No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13880.10749848394609277No Hit
GCCTGGTACCAGCAAAGGCCTGGCCAGGCTCCCAGGCTCCTCATTCATAGTGTTTCGACCAGGGCCGCTGGCGTC13870.10742103547062727No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13860.10734358699516179No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13670.1058720659613176No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13560.10502013273119726No Hit
GTCTGGTCCCCTGGCCAAAAGTGAACGGAGGTGAGCCACCAAATTCTTGA13420.10393585407468048No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13280.10285157541816368No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13200.1022319876144398No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13160.10192219371257788No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13140.1017672967616469No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12930.10014087877687171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3550.045.60072340
GGTATCA12050.041.442871
CGTATGC4050.040.9338841
AATCTCG3700.040.5174536
ATGCCGT4250.039.272544
ATCTCGT3750.039.14416537
TCTCCGA4350.037.7462111
GGCAATC4200.036.2993433
CTTATAC4350.036.212561
ACGAGAC4750.035.2894422
AGGCAAT4800.035.24410632
GTATCAA27750.035.1463851
ATCTCCG4700.034.93532610
ACGTACC2800.034.707513
CGTCTTC4700.034.4507948
CCGTCTT4800.034.35598447
ATACGTA2650.034.14164411
GCAATCT4450.033.55784634
CACGAGA5000.033.5171821
CCCACGA5050.033.18275519
Produced by FastQC (version 0.11.5)