FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291181
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56530.437816231806385No Hit
GTATTACTGTCAAGAATTTGGTGGCTCACCTCCGTTCACTTTTGGCCAGGGGACCAGACTGGAGATCAAACGAA53380.41341996203475734No Hit
GTGTATTACTGTCAAGAATTTGGTGGCTCACCTCCGTTCACTTTTGGCCA46980.3638529377368471No Hit
CTCCTGCAGGGTCAGTCTGAGTGTCTCCAACAACTACTTAGCCTGGTACC43970.34054094662173623No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42720.33085988718855064No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA41670.32272779726467477No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTTTGGCGATGGTGAGAGTGA40620.31459570734079884No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39720.3076253445489052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38910.301352018036201No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGAGAAGAAGCCGCCCTCTCCTGCAGGGTCAGTCTGAGTGTCTCCAA38060.2947688976216348No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36630.2836937656300705No Hit
GTCTGAGTGTCTCCAACAACTACTTAGCCTGGTACCAGCAAAGGCCTGGC36590.2833839717282085No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTTTGGCGATGGTGAGAGTGAAGTCTGCCCCAGACCCACTGCCA34970.2708373187028No Hit
GTGTTGACACAGTCTCCAGACACCCTGTCTTTGTCTCCAGGAGAAGAAGC33470.25922004738297727No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32290.2500811272780501No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31360.24287841905976No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29560.22893769347597276No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27960.2165459374014952No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25780.19966216975001957No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25700.19904258194629568No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25080.19424077646743562No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24880.19269180695812593No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24760.19176242525254011No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24500.18974876489043752No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24000.18587634111716328No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCTGGTCCCCTGGCCAAAAGTG23890.18502440788704294No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22670.17557569388025382No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22260.17240030638616893No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20910.1619447621983285No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20890.16178986524739755No Hit
GGCCTGGCCAGGCTCCCAGGCTCCTCATTCATAGTGTTTCGACCAGGGCC20880.16171241677193207No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT20720.1604732411644843No Hit
CATTCATAGTGTTTCGACCAGGGCCGCTGGCGTCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGGCAGACTTCA20330.1574527506213304No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT20280.15706550824400298No Hit
CACTCAGACTGACCCTGCAGGAGAGGGCGGCTTCTTCTCCTGGAGACAAA20150.15605867806295165No Hit
GAGTGAAGTCTGCCCCAGACCCACTGCCACGGAACCTGTCTGGGACGCCA20070.1554390902592278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCTGGTCC19790.15327053294619422No Hit
CTATGAATGAGGAGCCTGGGAGCCTGGCCAGGCCTTTGCTGGTACCAGGCTAAGTAGTTGTTGGAGACACTCAGA19710.15265094514247035No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19670.1523411512406084No Hit
GCCCCAGACCCACTGCCACGGAACCTGTCTGGGACGCCAGCGGCCCTGGT18620.1442090613167325No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18370.14227284943009538No Hit
GTCTGGGGCAGACTTCACTCTCACCATCGCCAAACTGGAGCCTGAAGATT18330.14196305552823346No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGACAAATGGTGTTGACACAGTC18050.13979449821519987No Hit
CTGCAGGAGAGGGCGGCTTCTTCTCCTGGAGACAAAGACAGGGTGTCTGG17820.13801318327949375No Hit
CTTTTGGCCAGGGGACCAGACTGGAGATCAAACGAACTGTGGCTGCACCA17700.13708380157390793No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17580.1361544198683221No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17570.13607697139285663No Hit
GTCTTTGTCTCCAGGAGAAGAAGCCGCCCTCTCCTGCAGGGTCAGTCTGA17400.13476034730994338No Hit
GACTTCACTCTCACCATCGCCAAACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAAGAATTTGGTGGCTCA17040.13197220219318592No Hit
GTCTGGGACGCCAGCGGCCCTGGTCGAAACACTATGAATGAGGAGCCTGGGAGCCTGGCCAGGCCTTTGCTGGTA17020.13181730524225496No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17010.13173985676678948No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16870.13065557811027267No Hit
GAGGTGAGCCACCAAATTCTTGACAGTAATACACTGCAAAATCTTCAGGC16650.12895171165003202No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACCGGACAAATGGTGTTGACACAGTCTC16560.12825467537084267No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16520.12794488146898073No Hit
GGCCAAAAGTGAACGGAGGTGAGCCACCAAATTCTTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTTTGG16360.12670570586153296No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16350.12662825738606748No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16030.12414990617117198No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15730.12182645190720744No Hit
GTCCCAGACAGGTTCCGTGGCAGTGGGTCTGGGGCAGACTTCACTCTCAC15660.12128431257894905No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15340.11880596136405353No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14890.1153207799681067No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14850.11501098606624478No Hit
CTCCCAGGCTCCTCATTCATAGTGTTTCGACCAGGGCCGCTGGCGTCCCA14770.1143913982625209No Hit
GGACCAGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14310.11082876839110861No Hit
GCCTGGGAGCCTGGCCAGGCCTTTGCTGGTACCAGGCTAAGTAGTTGTTG14200.10997683516098827No Hit
GCCTGGTACCAGCAAAGGCCTGGCCAGGCTCCCAGGCTCCTCATTCATAGTGTTTCGACCAGGGCCGCTGGCGTC14130.10943469583272987No Hit
CTCCAGACACCCTGTCTTTGTCTCCAGGAGAAGAAGCCGCCCTCTCCTGC14000.10842786565167857No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13640.10563972053492113No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13420.10393585407468048No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13400.1037809571237495No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13270.1027741269426982No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13270.1027741269426982No Hit
GGGTCAGTCTGAGTGTCTCCAACAACTACTTAGCCTGGTACCAGCAAAGG13250.10261922999176723No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13230.10246433304083626No Hit
GTCTGGTCCCCTGGCCAAAAGTGAACGGAGGTGAGCCACCAAATTCTTGA13200.1022319876144398No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13170.10199964218804336No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13150.10184474523711239No Hit
GCAAAGGCCTGGCCAGGCTCCCAGGCTCCTCATTCATAGTGTTTCGACCAGGGCCGCTGGCGTCCCAGACAGGTT12940.10021832725233719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCAT1351.8033461E-840.29963370
GGTATCA13650.037.73951
CACCGAG3600.034.00281570
GTATCAA31750.033.50361
ATTGCGT400.00302425333.443153
TAGACGG2450.032.7606355
GTAGACG2200.031.9230024
AATGGTA8150.031.70814770
TATCAAC34750.030.4116082
CGGCAGT2350.029.9445121
GTGCGAA450.00535914629.7537449
ATCAACG35500.029.6749043
CGGTCAA2650.029.42497833
TCAACGC36150.029.2338454
CAACGCA36400.029.2168165
AACGCAG36750.028.9464056
AGAGTAC37700.028.3265711
CGAAAAT500.007109210428.08131447
GTGGTAT4650.028.055611
CGCAGAG38000.027.920258
Produced by FastQC (version 0.11.5)