FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210179
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTCTGCTTTATTTGGGTAGTGACGTTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT33720.27863646617566495No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTCTTGAGTTGCAGATACA31950.2640105306735615No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28220.23318864399398767No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG27410.2264954192726861No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26380.21798428166411743No Hit
GTGGTGACCCAGGAGCCATCGCTCTCTGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGACCTCTGA25510.21079526251901576No Hit
GTATATTACTGTGCGAAAAACCCTAACTTCTATGGTTCGGCCATCTACTC25280.208894717227782No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT25020.20674627472464815No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAACGTCACTACCCAAATAAAGCAGA24120.19930935836764643No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC23460.19385561970584517No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT23290.1924508688384115No Hit
CTCCTGTGCCGCCTCTGGATTCCCGTTGTCATTTGACACCTATGCCATGA22470.18567501171314327No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22200.18344393680604273No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAACGTCACTACCCAAATAAAGCAGACAGTAATAAT22150.18303077478620933No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21570.17823809535614152No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC21350.17642018246887442No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21190.17509806400540748No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20860.17237119467450684No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTCGATTCTCAGACTGTGGTGACCCAGGAGCCAT20840.17220592986657346No Hit
CCCCAGGCCAGCCTCCACGCACCCTCATCTACAATACAAACATTCTCTCT20650.17063591419120644No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20500.16939642813170613No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19330.15972843686760388No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19180.1584889508081036No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19140.15815842119223686No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18520.15303521214630234No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18060.14923412156383478No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17680.1460940902131007No Hit
GTCTCTACTCGTCACGACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCC17370.14353248569013344No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17190.1420451024187331No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17060.14097088116716616No Hit
GTCTTGGTACAGCCGGGGGGGTCCCTGACACTCTCCTGTGCCGCCTCTGG16930.13989665991559927No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16920.13981402751163258No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16890.13956613029973253No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16820.1389877034719657No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16660.13766558500849874No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16550.1367566285648652No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16360.13518661288949818No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16250.13427765644586462No Hit
CTGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGACCTCTGAC16230.13411239163793126No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15980.13204658153876409No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15920.13155078711496399No Hit
CTTCTGGGGTCCCTAATCGCTTCTCTGGCTCCATCGTTGGGGACAAAGCTGCCCTCACCATCACGGGGGCCCAGG15850.1309723602871972No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15690.12965024182373022No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTCG15500.12808022614836317No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14530.12006488296359465No Hit
CTCATCTACAATACAAACATTCTCTCTTCTGGGGTCCCTAATCGCTTCTCTGGCTCCATCGTTGGGGACAAAG14380.11882539690409437No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14280.1179990728644275No Hit
CCCTCATCTACAATACAAACATTCTCTCTTCTGGGGTCCCTAATCGCTTCTCTGGCTCCATCGTTGGGGACAAAG14180.11717274882476064No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14130.1167595868049272No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14110.11659432199699384No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14010.11576799795732698No Hit
GTAGATGAGGGTGCGTGGAGGCTGGCCTGGGGTCTGCTGGTACCAGCTGG13860.11452851189782669No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTCTGC13770.11378482026212651No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT13730.11345429064625975No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC13690.11312376103039302No Hit
CCCCAGAAGAGAGAATGTTTGTATTGTAGATGAGGGTGCGTGGAGGCTGG13680.11304112862642633No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13670.11295849622245965No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13520.11171901016295938No Hit
GTCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC13490.1114711129510593No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13460.11122321573915925No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13450.11114058333519257No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13440.11105795093122588No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13330.11014899448759234No Hit
CTTGTGGCTTGACCTCTGACTCAGTCTCTACTCGTCACGACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCCTC13250.10948793525585884No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12900.10659580111702484No Hit
CACTTGTGGCTTGACCTCTGACTCAGTCTCTACTCGTCACGACCCCAGCTGGTACCAGCAGACCCCAGGCCAGC12890.10651316871305816No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTCGTGACGAGTAGAGACTGAG12720.10510841784562448No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12590.10403419659405758No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12320.10180312168695704No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12280.10147259207109031No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12260.10130732726315694No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12200.10081153283935682No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12110.10006784120365664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCA300.003047683564.65530470
GGTATCA11500.049.9241331
TCGTATG1700.046.6697840
CGTATGC1750.045.4007641
TCTCGTA1650.041.72688738
ATGCCGT1850.041.21372644
CTGTACG350.00133941139.47892441
TATCTCG1700.038.40748236
TCTGGAA9100.038.36688270
CTTATAC2050.036.6748161
ATCTCGT1900.036.20334237
TACACAT2300.035.6556745
GCCGTCT2050.035.59629446
GTATCAA28150.035.4489631
CGTCTTC2100.034.82486748
GCCTATC1900.034.29474333
CCCACGA2300.034.16578719
GTATGCC2350.033.84867542
TATACAC2550.033.5000233
AGACCGG2350.033.4540625
Produced by FastQC (version 0.11.5)