FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210179
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTCTTGAGTTGCAGATACA42850.35407985099724915No Hit
GATTATTACTGTCTGCTTTATTTGGGTAGTGACGTTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT31210.25789573278002675No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30310.25045881642302503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26920.2224464314783185No Hit
GTGGTGACCCAGGAGCCATCGCTCTCTGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGACCTCTGA25550.21112579213488253No Hit
GTATATTACTGTGCGAAAAACCCTAACTTCTATGGTTCGGCCATCTACTC24900.20575468587704795No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG24650.20368887577788078No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT23910.19757407788434603No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23890.19740881307641267No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23650.19542563538121221No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAACGTCACTACCCAAATAAAGCAGA23120.19104611797097784No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22360.18476605526950973No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC21960.18146075911084228No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20690.17096644380707315No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC20540.16972695774757288No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTCGATTCTCAGACTGTGGTGACCCAGGAGCCAT20520.16956169293963952No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20370.1683222068801392No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20150.16650429399287212No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19500.16113318773503754No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAACGTCACTACCCAAATAAAGCAGACAGTAATAAT19280.15931527484777044No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18740.1548531250335694No Hit
CCCCAGGCCAGCCTCCACGCACCCTCATCTACAATACAAACATTCTCTCT18700.1545225954177027No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18170.15014307800746832No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17830.14733357627260102No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17620.1455982957893006No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17620.1455982957893006No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17610.14551566338533392No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT17460.14427617732583362No Hit
GTCTCTACTCGTCACGACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCC17310.14303669126633334No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16550.1367566285648652No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC16460.13601293692916502No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16200.13386449442603118No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15950.13179868432686404No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15570.12865865297613No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15410.127336534512663No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTCG15360.1269233724928296No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15340.1267581076848962No Hit
CTTCTGGGGTCCCTAATCGCTTCTCTGGCTCCATCGTTGGGGACAAAGCTGCCCTCACCATCACGGGGGCCCAGG15220.12576651883729598No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15120.1249401947976291No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14670.12122173661912825No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14610.12072594219532813No Hit
CTCCTGTGCCGCCTCTGGATTCCCGTTGTCATTTGACACCTATGCCATGA14370.11874276450012766No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14050.11609852757319371No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14040.11601589516922703No Hit
CTCATCTACAATACAAACATTCTCTCTTCTGGGGTCCCTAATCGCTTCTCTGGCTCCATCGTTGGGGACAAAG14030.11593326276526034No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT13960.11535483593749356No Hit
CCCTCATCTACAATACAAACATTCTCTCTTCTGGGGTCCCTAATCGCTTCTCTGGCTCCATCGTTGGGGACAAAG13950.11527220353352687No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13810.11411534987799325No Hit
CTGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGACCTCTGAC13610.11246270179865953No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13490.1114711129510593No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13460.11122321573915925No Hit
CTTGTGGCTTGACCTCTGACTCAGTCTCTACTCGTCACGACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCCTC13350.1103142592955257No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12980.10725686034875834No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12960.10709159554082495No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTCGTGACGAGTAGAGACTGAG12910.10667843352099153No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12620.10428209380595763No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12400.10246418091869054No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12360.1021336513028238No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12350.10205101889885712No Hit
GTAGATGAGGGTGCGTGGAGGCTGGCCTGGGGTCTGCTGGTACCAGCTGG12300.10163785687902367No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12250.10122469485919025No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12210.1008941652433235No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTCTGC12210.1008941652433235No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12200.10081153283935682No Hit
GTCTTGGTACAGCCGGGGGGGTCCCTGACACTCTCCTGTGCCGCCTCTGG12170.10056363562745677No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12130.10023310601159002No Hit
CACTTGTGGCTTGACCTCTGACTCAGTCTCTACTCGTCACGACCCCAGCTGGTACCAGCAGACCCCAGGCCAGC12120.10015047360762334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACA150.002460164768.2481411
GCCTTAC352.4162018E-548.7789041
GTGGTAT3150.044.4430051
GGTATCA10950.043.344181
TGGTATC3400.042.165462
TCTGGAA14450.040.39444770
GTATCAA27850.037.2716371
TATCAAC32250.032.070032
ATCAACG33000.031.3333953
TCAACGC33650.030.7281444
CAACGCA33950.030.4578725
AACGCAG33900.030.3014586
AAAGGGA14400.029.01916570
CGCAGAG35400.029.0174988
AATACGT953.5251833E-728.73724710
TTAACGG1202.8230716E-928.61527435
AGAGTAC36000.028.43672611
GCAGAGT36650.028.1209329
CAGAGTA36650.028.02665710
CCGACAC500.007208420428.00161251
Produced by FastQC (version 0.11.5)