FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569581
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG97090.6185727273711902No Hit
GATTATTACTGTGCAGCATGGGATGACACCCTGAGTGTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGT87040.5545429003026923No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC73160.4661116565503787No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACACTCAGGGTGTCATCCCATGCTGCA65770.4190290274920504No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACACTCAGGGTGTCATCCCATGCTGCACAGTAATAAT61300.3905500894824797No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCGTCAGTGTCTGGGACCC60720.38685483578101415No Hit
GTCCTGACTCAGCCACCGTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC53860.3431489040705768No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG52100.3319357204247503No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50880.32416294539752966No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49580.3158804802045896No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCGTCAGTGTCT46860.2985510145701305No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC43580.27765371777563563No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC42900.27332135136702085No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT39230.24993931501464403No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37500.2389172651809623No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCAC35110.22369027148009565No Hit
ACCATATACTACGCAGCCTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACAATGCCAAAAGCTCCCTGTATCTG34350.21884821490576145No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34130.21744656695003314No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG34100.21725543313788842No Hit
GGATGACACCCTGAGTGTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCC34070.21706429932574364No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33580.21394244706071236No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG33180.21139399623211547No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA32680.20820843269636927No Hit
GAACTAATGCTGTCTACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAA31470.2004993689398636No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31250.19909772098413528No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA30960.1972500941334025No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30410.19374597424408171No Hit
GCATGGGATGACACCCTGAGTGTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC30240.19266288264192802No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30010.19119752341548477No Hit
GTATATGGTCGCACTACTCCCAGTAATATATGAAATCCATTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATG29890.19043298816690568No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACACTCAGGGTGT29620.18871278385760276No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG29480.18782082606759384No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA29390.18724742463115954No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT29280.18654660065329537No Hit
GTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG28560.1819593891618209No Hit
GTCTACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAACTCCTCATCTT28520.1817045440789612No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG28430.1811311426425269No Hit
CCCCTGGAGGCAGAGATTCGGTCAGGGACACCTGAGGGACGCTGATTATT28260.1800480510403732No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27980.17826413546035536No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27540.1754608395488987No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26930.1715744520352884No Hit
CCAGTAGACAGCATTAGTTCCGATGTTGGAGTTGCTTCCAGAACAAGAGA26400.16819775468739745No Hit
CCTGAGGGACGCTGATTATTGCTAAAGATGAGGAGTTTGGGGGCCGTTCC26220.16705095181452886No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25710.16380167700806775No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25640.16335569811306327No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAACTCCAACATCGGAACTAATGCTGTCTA25360.16157178253304544No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC24590.15666601468799635No Hit
ATAATATACAGCCGTGTCGTCGCCTCTCAGGCCATTCATTTGCAGATACA24530.1562837470637068No Hit
GCATTAGTTCCGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGACCCT24260.1545635427544039No Hit
TCCCTCAGGTGTCCCTGACCGAATCTCTGCCTCCAGGGGTGGCACGTCAG24170.15399014131796956No Hit
AGTCTGTCCTGACTCAGCCACCGTCAGTGTCTGGGACCCCCGGGCAGAGG24050.15322560606939048No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23670.15080457778222342No Hit
CCTCAGGTGTCCCTGACCGAATCTCTGCCTCCAGGGGTGGCACGTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT23350.14876581711934586No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT22740.14487942960573555No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22440.14296809148428785No Hit
CACCTGAGGGACGCTGATTATTGCTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAGTAGA22210.1415027322578446No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC22070.14061077446783568No Hit
CTGATGGCCAGGGAGGCTGACGTGCCACCCCTGGAGGCAGAGATTCGGTC21930.13971881667782676No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT21470.13678809822494029No Hit
CTCTTGTTCTGGAAGCAACTCCAACATCGGAACTAATGCTGTCTACTGGTACCAGCAAGTCCCAGGAACGGCCCC21220.1351953164570672No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG21100.13443078120848811No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20970.1336025346891941No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACA20860.13290171071132997No Hit
CTCCAGGGGTGGCACGTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCG20270.1291427457391495No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20250.12901532319771963No Hit
GTATATTATTGTGCGAGACCCGGCGACAGTAATGGTTATTACTACAGACCCCCTGACTTCTGGGGCCAGGGCACC20170.12850563303200027No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19770.12595718220340332No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19570.12468295678910488No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19500.1242369778941004No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19450.12391842154052578No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19420.123727287728381No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19310.12302646375051687No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19200.1223256397726527No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19170.12213450596050793No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19120.12181594960693332No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19120.12181594960693332No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC18900.120414301651205No Hit
GTCCCAGGAACGGCCCCCAAACTCCTCATCTTTAGCAATAATCAGCGTCCCTCAGGTGTCCCTGACCGAATCT18540.11812069590546777No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGACGTGCCACCC18240.11620935778402007No Hit
GTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAGTAGACAGCATTAGTT18090.11525368872329622No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17860.11378832949685297No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17830.11359719568470822No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17700.11276894916541422No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17660.11251410408255451No Hit
GTGCAGCATGGGATGACACCCTGAGTGTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGG17610.11219554772897991No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17490.11143101248040081No Hit
CTACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAACTCCTCATCTTTAGCAATAATCAGCGTCCCTCAGGTGT17490.11143101248040081No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17480.11136730120968591No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17390.11079389977325159No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC17280.11009307579538744No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16820.10716235734250096No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16750.10671637844749648No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16600.10576070938677265No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16470.10493246286747863No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16440.10474132905533388No Hit
GTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAACTCCAACATCGGAACTAATG16440.10474132905533388No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16340.10410421634818463No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG16310.10391308253603988No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGACGTGCCACCCCTGGAGGCAGAGATTCGGTCAGGGACA16300.10384937126532495No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16300.10384937126532495No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC16100.10257514585102648No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16010.10200174441459217No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCGTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC15910.10136463170744295No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCGTCAGTGTCTGGGA15850.1009823640831534No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15820.10079123027100863No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC15820.10079123027100863No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15740.10028154010528925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)