FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569581
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACACCCTGAGTGTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGT82040.5226872649452305No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC68880.43884323268439157No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCGTCAGTGTCTGGGACCC68170.43431973246363204No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG57990.3694616588758401No Hit
GTCCTGACTCAGCCACCGTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC57940.3691431025222655No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACACTCAGGGTGTCATCCCATGCTGCA56930.36270826418005825No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53940.3436585942362962No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCGTCAGTGTCT53700.342129523739138No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACACTCAGGGTGTCATCCCATGCTGCACAGTAATAAT52290.33314623456833387No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48450.3086811066138033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44910.28612731678072045No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG44660.2845345350128474No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC40880.2604516746826064No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC40560.2584129140197288No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA40060.25522735048398265No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCAC39050.24879251214177542No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37970.2419116949045637No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35650.2271306800987015No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG35180.22413625037510013No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35100.22362656020938074No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34370.21897563744719134No Hit
GAACTAATGCTGTCTACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAA32810.2090366792156633No Hit
GGATGACACCCTGAGTGTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCC32530.20725276363564543No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32140.20476802407776346No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31850.20292039722703067No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31510.20075421402272328No Hit
GTCTACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAACTCCTCATCTT31120.19826947446484125No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG30850.19654927015553833No Hit
ACCATATACTACGCAGCCTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACAATGCCAAAAGCTCCCTGTATCTG30780.19610329126053386No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC29890.19043298816690568No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29270.18648288938258045No Hit
GCATGGGATGACACCCTGAGTGTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC29160.18578206540471628No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAACTCCAACATCGGAACTAATGCTGTCTA28440.1811948539132418No Hit
GTATATGGTCGCACTACTCCCAGTAATATATGAAATCCATTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATG28100.1790286707089344No Hit
CCCCTGGAGGCAGAGATTCGGTCAGGGACACCTGAGGGACGCTGATTATT27820.17724475512891658No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27650.17616166352676288No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27350.17425032540531518No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27020.1721478534717227No Hit
AGTCTGTCCTGACTCAGCCACCGTCAGTGTCTGGGACCCCCGGGCAGAGG26670.1699179589967004No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC26600.16947198010169592No Hit
CTCTTGTTCTGGAAGCAACTCCAACATCGGAACTAATGCTGTCTACTGGTACCAGCAAGTCCCAGGAACGGCCCC25800.16437507844450208No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25290.16112580363804097No Hit
GTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG25090.15985157822374252No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25010.15934188805802313No Hit
CCTGAGGGACGCTGATTATTGCTAAAGATGAGGAGTTTGGGGGCCGTTCC24720.15749426120729035No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24130.1537352962351099No Hit
CCTCAGGTGTCCCTGACCGAATCTCTGCCTCCAGGGGTGGCACGTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT24030.15309818352796065No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACACTCAGGGTGT23600.15035859888721895No Hit
TCCCTCAGGTGTCCCTGACCGAATCTCTGCCTCCAGGGGTGGCACGTCAG23550.1500400425336443No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23220.14793757060005186No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23170.14761901424647725No Hit
CCAGTAGACAGCATTAGTTCCGATGTTGGAGTTGCTTCCAGAACAAGAGA23000.14653592264432355No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT22940.14615365502003402No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC22560.14373262673286694No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22480.14322293656714755No Hit
ATAATATACAGCCGTGTCGTCGCCTCTCAGGCCATTCATTTGCAGATACA22420.142840668942858No Hit
GCATTAGTTCCGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGACCCT22350.14239469004785354No Hit
CTCCAGGGGTGGCACGTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCG21390.1362784080592209No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21080.13430335866705828No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCACA20870.1329654219820449No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20830.1327105768991852No Hit
CTGATGGCCAGGGAGGCTGACGTGCCACCCCTGGAGGCAGAGATTCGGTC20730.13207346419203597No Hit
CACCTGAGGGACGCTGATTATTGCTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAGTAGA20640.13150006275560164No Hit
GTCCCAGGAACGGCCCCCAAACTCCTCATCTTTAGCAATAATCAGCGTCCCTCAGGTGTCCCTGACCGAATCT20310.1293975908220092No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC20310.1293975908220092No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20270.1291427457391495No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG20250.12901532319771963No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20210.12876047811485994No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19980.12729511888841671No Hit
GTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAGTAGACAGCATTAGTT19910.12684913999341227No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC19800.1261483160155481No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19740.12576604839125857No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19700.12551120330839888No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19670.1253200694962541No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19390.12353615391623625No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19340.12321759756266162No Hit
GTATATTATTGTGCGAGACCCGGCGACAGTAATGGTTATTACTACAGACCCCCTGACTTCTGGGGCCAGGGCACC19230.12251677358479747No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19160.122070794689793No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19150.1220070834190781No Hit
GTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAACTCCAACATCGGAACTAATG19120.12181594960693332No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18940.1206691467340647No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18860.1201594565683453No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18560.11824811844689762No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCGTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC18140.11557224507687082No Hit
CTACTGGTACCAGCAAGTCCCAGGAACGGCCCCCAAACTCCTCATCTTTAGCAATAATCAGCGTCCCTCAGGTGT18090.11525368872329622No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAACTCCAACATCGGAACTAATGCT17880.11391575203828282No Hit
GTGCAGCATGGGATGACACCCTGAGTGTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGG17840.11366090695542314No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC17820.11353348441399329No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17540.11174956883397544No Hit
GCCCCCAAACTCCTCATCTTTAGCAATAATCAGCGTCCCTCAGGTGTCCC17450.11117616739754113No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG17220.10971080817109789No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17210.10964709690038296No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCGTCAGTGTCTGGGA16810.10709864607178604No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16770.10684380098892633No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16740.10665266717678158No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16730.10658895590606665No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16590.10569699811605773No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16500.10512359667962341No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACACCCTGA16480.10499617413819358No Hit
CTCCTCATCTTTAGCAATAATCAGCGTCCCTCAGGTGTCCCTGACCGAAT16060.10232030076816678No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15920.10142834297815788No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15910.10136463170744295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGAT651.2895802E-554.23653470
CGTTTGA250.006751682652.88982469
TGGTATC3150.043.0182082
GTGGTAT3100.042.6301161
GGTATCA9300.040.8083151
TCGAACG350.001254944240.0061749
CATTCGA350.001289025839.78874246
GTACGTA450.001390812739.1776569
GTATCAA23550.038.9934771
CGAACGT400.002393349535.07814450
ACGGGTA601.5130832E-533.90384316
TATCAAC27200.033.378652
AAAACCG1751.2882447E-732.23270
ATCAACG28300.032.0802273
TCAACGC28450.031.9110854
CAACGCA29450.031.1755835
AACGCAG29750.030.9790336
CGACCCA450.00460338630.6935741
ACGCAGA31350.029.1873847
AATGGTA4350.029.17551470
Produced by FastQC (version 0.11.5)