FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118426
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGACCTCCGACCCTCACTTTCGGCGGAGGGA63460.567404548892819No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43960.39305237896830014No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA37500.3352926344702287No Hit
GTATGGACCTCCGACCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT37140.3320738251793145No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36970.33055383190304943No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30290.2708270372827527No Hit
GTGTATTACTGTCAGCAGTATGGACCTCCGACCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA29610.26474706417769256No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28450.25437534535141354No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26360.23568836919027275No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25510.22808840280894754No Hit
GGTCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTC25160.22495900488722545No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24960.2231707775033842No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTAGAGCCTGAAGATT24220.21655433618317171No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24170.2161072793372114No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCGGCAACTACTTAGCCTGGTACC22370.20001323288264045No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21850.19536384168465326No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21450.1917873869169708No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21170.18928386857959312No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20510.18338271821291707No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20000.17882273838412197No Hit
GTAATACACTGCAAAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19990.17873332701492992No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGGGGCCCTGCT19890.17783921332300928No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19160.17131218337198884No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCGG18480.1652322102669287No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18450.16496397615935252No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17780.15897341442348442No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17330.15494990280984167No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16420.14681346821336413No Hit
GTCAGAGTGTTACCGGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGC16290.14565112041386732No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16240.14520406356790705No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15990.1429687793381055No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15480.1384087995093104No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT15020.13429587652647562No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15000.13411705378809147No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14740.1317923581890979No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA14320.12803708068303132No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14290.12776884657545515No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14280.1276794352062631No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14160.12660649877595834No Hit
CTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGTTGCATCCAGCAGGGCCC13900.12428180317696476No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13830.12365592359262034No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13420.11999005745574585No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA13280.118738298287057No Hit
GAGTGTTACCGGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG13240.11838065281028876No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13130.11739712774917607No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12720.11373126161230158No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12420.11104892053653975No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12340.11033362958300325No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12210.10917128178350645No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11800.10550541564663196No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11760.10514777016986372No Hit
GTGTATTACTGTGCGAAAGATTATCCGTCTAGGATTACAAGGGGCTACTT11600.10371718826279075No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11560.1033595427860225No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11540.10318072004763838No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11440.10228660635571776No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11380.10175013814056541No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11370.10166072677137335No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11280.10085602444864479No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11280.10085602444864479No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11190.10005132212591625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1600.051.852741
GTCGCTA200.00741483251.65109338
TTCGCAT200.007688912651.17907727
ACGTGAT400.00827042850.24056670
TCGTATG1650.050.2130340
GGTATCA11450.048.7779121
TATCTCG1550.048.7683236
ATCTCGT1800.042.03666337
TAACGGC503.951982E-641.231436
TTAACGG503.9876577E-641.1775335
ACGAGAC2100.040.55109822
ATGCCGT1800.040.47684544
GTATCAA32400.040.151631
AGACCGG2150.039.6435125
CACGAGA2250.039.3580821
TCTCCGA2350.039.12737711
GAGCCTA2100.039.10569831
GACCGGA2200.038.74946226
AGCCTAT2050.038.41451332
CGCGGTA557.179142E-637.79854643
Produced by FastQC (version 0.11.5)