FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118426
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGACCTCCGACCCTCACTTTCGGCGGAGGGA53120.474953193148228No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43930.39278414486072394No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA34340.3070386418055374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31180.27878464914084616No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30360.27145291686709716No Hit
GTATGGACCTCCGACCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT29810.2665352915615338No Hit
GTGTATTACTGTCAGCAGTATGGACCTCCGACCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26420.2362248374054251No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25500.22799899143975552No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25410.227194289117027No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25140.22478018214884132No Hit
GGTCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTC23530.2103849517089195No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23310.20841790158669415No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTAGAGCCTGAAGATT23190.20734496515638942No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCGGCAACTACTTAGCCTGGTACC20650.18463447738160593No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20550.18374036368968533No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19870.17766039058462518No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19640.17560392909320777No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18430.1647851534209684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18330.16389103972904778No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18270.16335457151389543No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18200.16272869192955097No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17890.1599569394845971No Hit
GTAATACACTGCAAAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17860.15968870537702093No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17810.1592416485310606No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17600.15736400977802734No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17400.15557578239418612No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGGGGCCCTGCT17130.15316167542600045No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16270.14547229767548323No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCGG16150.1443993612451785No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15910.14225348838456903No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15720.1405546723699199No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15030.13438528789566764No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14770.13206059229667408No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14640.13089824449717727No Hit
GTCAGAGTGTTACCGGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGC13920.12446062591534889No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA13880.12410298043858065No Hit
GAGTGTTACCGGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG13880.12410298043858065No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13740.12285122126989179No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13310.11900653239463317No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA13260.11855947554867285No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT13020.1164136026880634No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13010.11632419131887134No Hit
CTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGTTGCATCCAGCAGGGCCC12960.11587713447291104No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12710.11364185024310952No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12280.10979716136785089No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11990.10720423166128114No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11930.10666776344612876No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11790.1054160042774399No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11720.10479012469309548No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11410.10201837224814159No Hit
GTGTATTACTGTGCGAAAGATTATCCGTCTAGGATTACAAGGGGCTACTT11270.10076661307945273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGC200.007047193652.3196347
GGTATCA11550.043.554341
GTATCAA31750.040.4668851
TACGTAT700.001309220239.658670
TATCAAC36500.034.438712
AACTGTG22400.033.77177470
ATCAACG37300.033.6910323
TCAACGC37700.033.24054
CAACGCA38450.032.6804475
AACGCAG39300.032.060036
AATGGTA5000.031.9251770
CGACTTC603.5428978E-430.9163967
CGCAGAG42150.030.0534158
AGAGTAC42850.029.55849611
ACGGGAC1052.6951966E-829.13301716
TGTTAGA12200.029.01254370
GCAGAGT44050.028.8342259
GCGTGTT4900.028.64294651
GTATAGG605.9156737E-428.3250351
CGTGTTC5050.027.8397552
Produced by FastQC (version 0.11.5)