FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042022
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47540.4562283713779556No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35830.3438507056472896No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33770.3240814493360025No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33440.32091452963565065No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA30040.28828565999566225No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29270.2808961806948414No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28280.27139542159378593No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26570.2549850195101447No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25890.24845924558214702No Hit
GGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC25520.2449084568272071No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACATCTATTTAAGTTGGTATCAACAAAAA24980.2397262245902678No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24670.23675123941721No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTCCAGCCTGAAGATT24620.23627140309897487No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23520.22571500409780215No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGAAGAGGTACTGAAACTCTGTTGA23360.22417952787944975No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22230.21333522708733596No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22120.2122795871872187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21640.2076731585321615No Hit
GATTTCACTCTCACCATCAGCAGTCTCCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTAC21560.2069054204229853No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21290.20431430430451564No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20970.20124335186781084No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20600.19769256311287092No Hit
ACTTACTACTGTCAACAGAGTTTCAGTACCTCTTCGATCACCTTCGGCCA20420.1959651523672245No Hit
GCATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTTTGTTGATACCAACT20320.19500547973075424No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19550.18761600042993334No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19480.18694422958440418No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19130.1835853753567583No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19050.1828176372475821No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT18230.17494832162852608No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18190.17456445257393796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18130.17398864899205582No Hit
GTACTGAAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGGAGACTGCTGATGGTGAGAGTGAAAT17990.17264510730099747No Hit
GTAGTAAGTTGCAAAATCTTCAGGCTGGAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA17460.16755884232770515No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG17050.16362418451817717No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16890.16208870829982477No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16850.16170483924523665No Hit
GATCAGGAGCTTAGGGGCTTTTCCTGGTTTTTGTTGATACCAACTTAAAT16250.1559468034264152No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16240.15585083616276815No Hit
CCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCA16070.15421939268076873No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT15910.15268391646241633No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCC15870.15230004740782824No Hit
GTTTCAGTACCTCTTCGATCACCTTCGGCCAAGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCAT15700.15066860392582881No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15470.14846135686194725No Hit
CCCTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15010.1440468627341841No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14970.143662993679596No Hit
CGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT14860.14260735377947872No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14800.14203155019759658No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14680.1408799430338323No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14500.13915253228818586No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14330.13752108880618644No Hit
GAGTGAAATCTGTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCA14330.13752108880618644No Hit
GGCCGAAGGTGATCGAAGAGGTACTGAAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGGAGACTG14230.13656141616971618No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14160.13588964532418701No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACT14130.13560174353324594No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA14050.13483400542406973No Hit
GTCTCCCTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAT13880.13320256194207034No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13790.1323388565692471No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13500.1295558059234834No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13370.12830823149607207No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTCCAGCCTGAAGATTTTGCAACTTA13300.12763646065054288No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13240.12706065706866074No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13000.12475744274113214No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12990.12466147547748511No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12900.12379777010466189No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12690.12178245756807438No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12620.1211106867225452No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12620.1211106867225452No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12500.1199590795587809No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCCTCCTCC12480.11976714503148685No Hit
CACCATCAGCAGTCTCCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTACCTCTTCGATCAC12470.11967117776783982No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12420.1191913414496047No Hit
GTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGAAGAGGTACTGAAACTCTGTTGACAGTAGTAAGTTGCAAAAT12120.11631232354019398No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCA12040.11554458543101777No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11890.11410507647631239No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11680.11208976393972488No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11510.11045832045772545No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11440.10978654961219629No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11420.10959461508490223No Hit
CATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTCCAGCCTGAAGATTTTG11270.10815510613019688No Hit
AGTCAGAGCATTAACATCTATTTAAGTTGGTATCAACAAAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATG11070.10623576085725638No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10910.10470028463890398No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10860.10422044832066886No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10700.10268497210231646No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10690.10258900483866944No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10530.10105352862031705No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10480.10057369230208192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT150.002434364868.4291135
GCGAAAC150.00256552567.5294113
CCTAACG401.4128091E-859.1109282
CTAACGC553.1359377E-949.124093
GTGGTAT2900.041.9510421
TCACTCG400.002362870135.1687650
CGGGTAC808.5106876E-833.79227417
CATCTTA850.003365557932.71736570
ATCAACG34200.032.686553
TCAACGC34550.032.3507654
AACGCAG35150.031.7024846
CAACGCA35200.031.657455
AATGGTA5950.031.54888770
ATGTACC751.97392E-631.5152411
ACGCAGA38000.029.413667
CGCAGAG38500.029.2947948
CTAATGG950.005799541729.27343470
ACGGGTA1401.8189894E-1128.96341516
AGAGTAC38900.028.81856311
ACCCTGG5850.028.52283170
Produced by FastQC (version 0.11.5)