FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393441
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45480.326386262496941No Hit
GCCTTGGGCTGACCTAGGACGGCCACCTTGGTCCCTCCGCCGAATACCAGACTACTGCTGCTTATATATGAGCTG35550.2551238265559862No Hit
ATTTATTACTGTGCGAGGCGTTATTGCAGTGCTGGTAGTTGCTACTACGACTACTGGTACTTCGATCTCTGGGGC34140.24500499124110744No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG33130.23775674750491768No Hit
GTAATAAATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA32810.23546027424196647No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32670.2344555671894253No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32050.23000615024245732No Hit
GACCTAGGACGGCCACCTTGGTCCCTCCGCCGAATACCAGACTACTGCTGCTTATATATGAGCTGCAGTAATAAT31170.22369084876934153No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30290.21737554729622569No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT29640.212710835980856No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28590.20517553308679737No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25400.18228256524675246No Hit
CTATTATAGTGGGCGCACCTTCTACAACCCGTCCCTCAAGAGTCGACTCACCATATCCGTAGACACGTCCAAGAA25210.18091903424687517No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCACCTTGGTCCCTC24650.17690020603671056No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23740.17036961019519306No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23430.16814490172170907No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAAAGC23200.16649431156396288No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22980.1649154861956839No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22900.1643413678799461No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22440.16104018756445376No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21620.15515547482814127No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21000.15070605788117328No Hit
GATTATTACTGCAGCTCATATATAAGCAGCAGTAGTCTGGTATTCGGCGGAGGGACCAAGGTGGCCGTCCTAGGT20830.14948605646023044No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20710.14862487898662377No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT20360.14611311135527089No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20110.14431899161859024No Hit
CTATAATAGAAACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAAGAGTAACTGTCA20060.1439601676712541No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAGTA19920.14295546061871295No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19880.14266840146084403No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19700.141376635250434No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19670.14116134088203233No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19470.1397260450926878No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19200.13778839577707272No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18640.1337695675669081No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18610.13355427319850643No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18580.13333897883010476No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18550.13312368446170308No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18370.13183191825129303No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18240.1308989759882191No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18080.12975073935674347No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17430.12508602804137384No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17060.12243073083108649No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAATCGGCCCTCAGGGGTTTCAAA16920.12142602377854535No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16840.12085190546280754No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16780.1204213167260042No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC16390.1176224899367824No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16370.11747896035784794No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16350.11733543077891351No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16320.11712013641051182No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16110.11561307583170008No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16040.1151107223054295No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15850.11374719130555222No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15590.11188130677940436No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14980.10750365462190363No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14970.1074318898324364No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14790.10614012362202634No Hit
GCTTATATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC14690.10542247572735408No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14670.10527894614841962No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14610.10484835741161629No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14480.10391541514854234No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14400.10334129683280455No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14320.10276717851706674No Hit
GCTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAT14210.10197776583292728No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14130.10140364751718946No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14120.10133188272772224No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC13940.10004011651731219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT10750.051.56572770
GTGGTAT3850.043.2540441
TGGTATC4300.039.5064662
GGTATCA11150.038.4049341
GTATCAA28850.038.1672171
ACCGTAT451.0897413E-437.7885938
TACGTAT750.00185234236.95543770
CTACGGA11000.033.01775469
TATCAAC33250.032.6982842
ATCAACG34350.031.845573
TCAACGC35100.031.2618944
CAACGCA35400.030.9032175
AACGCAG36050.030.260466
CTACCGT450.00497655330.208096
TCTACGG11200.029.16699268
ACGCAGA38050.028.6801917
GACGTGT10800.028.5808462
CGCAGAG38250.028.5415048
GTCTACG11100.028.25853367
ACGTGTC10950.028.25681163
Produced by FastQC (version 0.11.5)