FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189234
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37380.31431997403370576No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACAGTGCTGGTGCTTGTATATGAGCTA31680.26638996194188863No Hit
GTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG30690.2580652756312046No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27260.2292231806356024No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25630.215516878932153No Hit
GAATAACACAGTGCTGGTGCTTGTATATGAGCTACAGTAATAATCAGCCT22760.19138369740522052No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22700.19087917096214874No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC21140.177761483442283No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGGCTATGGC20920.17591155315101992No Hit
GATTATTACTGTAGCTCATATACAAGCACCAGCACTGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT20700.1740616228597568No Hit
GTGCTGGTGCTTGTATATGAGCTACAGTAATAATCAGCCTCGTCCTCAGC20480.17221169256849367No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19950.1677550423213598No Hit
GCGATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCACTTGCAGATACA18870.15867356634606816No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18780.1579167766814605No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCACTGCATGCCATAGCCAC18570.15615093413070935No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT18370.15446917932047016No Hit
CACCAGCACTGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA18050.15177837162408744No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16930.14236054468674794No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16490.13866068410422172No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16460.13840842088268582No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16290.1369789292939825No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16260.13672666607244663No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16100.13538126222425528No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15980.13437220933811175No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGC15880.13353133193299216No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGTAGCTCATAT15690.13193366486326494No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC15520.13050417327456162No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15270.12840197976176262No Hit
GGGCTGGAGTGGGTGGCTCTTATATGGCATGATGGAAGTAAAAAATACTA15080.12680431269203538No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14790.12436576821718853No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC14650.1231885398500211No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14520.12209539922336562No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14490.12184313600182976No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14400.12108634633722212No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14340.12058181989415034No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG14330.1204977321536384No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14320.12041364441312642No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14300.12024546893210251No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14150.11898415282442312No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13850.11646152060906431No Hit
GCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA13780.11587290642548059No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13740.11553655546343276No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13520.11368662517216965No Hit
GTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAA13430.11292983550756201No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13340.11217304584295437No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCC13180.11082764199476301No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC13010.1093981504060597No Hit
AGGTAGGAGAAATAACCACTAACATCTGCATCTCTCGCACAGCGATACAC12960.1089777117034999No Hit
GTGTATCGCTGTGCGAGAGATGCAGATGTTAGTGGTTATTTCTCCTACCT12330.10368018405124643No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12310.10351200857022251No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12290.10334383308919859No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12220.10275521890561487No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12150.10216660472203115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAG400.00973827548.20625370
GGTATCA9350.046.8841171
CGTATGC1900.036.39426841
GTATCAA22500.036.3783261
GTACTAG1800.034.30798330
CGGGTAT601.4131016E-534.243066
TCGTATG1950.033.6523140
GACCGTA1900.032.45580726
TGCCGTC2250.032.3809545
GCCGTCT2150.032.30916646
TATCAAC25400.032.2208332
TACTAGA2150.031.92650831
ACCGTAC1950.031.63691927
GTGGTAT1950.031.6129671
ATGCCGT2200.031.51706144
ATCAACG26200.031.3676873
CGTACTA2000.030.86678329
TATACAC2450.030.748873
TCAACGC26750.030.5947364
ACTAGAT2250.030.52427932
Produced by FastQC (version 0.11.5)