FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189234
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34280.2882527744749982No Hit
GTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG32000.2690807696382714No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACAGTGCTGGTGCTTGTATATGAGCTA28400.23880918305396584No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27120.22804595226843496No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26560.22333703879976524No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24020.20197875270972745No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22870.19230866255085208No Hit
GTGCTGGTGCTTGTATATGAGCTACAGTAATAATCAGCCTCGTCCTCAGC22620.19020646903805308No Hit
GAATAACACAGTGCTGGTGCTTGTATATGAGCTACAGTAATAATCAGCCT21280.17893871180945045No Hit
GATTATTACTGTAGCTCATATACAAGCACCAGCACTGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT20790.17481841252436442No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20710.17414571060026873No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19240.16178481274501066No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGGCTATGGC19240.16178481274501066No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18630.1566554605737811No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18300.15388056513688644No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC18060.1518624593645994No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17030.14320142209186754No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16930.14236054468674794No Hit
GCGATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCACTTGCAGATACA16920.14227645694623597No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16910.142192369205724No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT16730.14067878987650873No Hit
CACCAGCACTGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA16480.13857659636370975No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCACTGCATGCCATAGCCAC16110.13546534996476722No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG15820.1330268054899204No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15790.1327745422683845No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15620.13134505067968122No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC15410.12957920812893003No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGTAGCTCATAT14890.12520664562230815No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGC14820.12461803143872442No Hit
GGGCTGGAGTGGGTGGCTCTTATATGGCATGATGGAAGTAAAAAATACTA14460.12159087278029387No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14200.11940459152698292No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14190.11932050378647095No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13980.1175546612357198No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13790.11595699416599256No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG13690.11511611676087295No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13560.11402297613421748No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13470.11326618646960984No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCC13470.11326618646960984No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13460.1131820987290979No Hit
GTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAA13130.1104072032922032No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12920.10864136074145206No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12910.10855727300094009No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12820.10780048333633245No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12760.1072959568932607No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC12410.10435288597534212No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12210.10267113116510293No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12130.10199842924100723No Hit
GCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA12050.10132572731691156No Hit
AGGTAGGAGAAATAACCACTAACATCTGCATCTCTCGCACAGCGATACAC11950.10048484991179195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTACG200.007589131651.3486631
TGGTATC3650.045.942352
TACGTAT607.98227E-443.855170
GTGGTAT3950.043.3322071
GGTATCA11750.041.078931
GGATGGG11450.040.21646570
GTACGTA500.002792344433.99107769
GGTACGT450.00288014333.77744768
GTATCAA23800.032.6502721
ACTTTCG1053.2788725E-431.32507170
CGTAGTA450.004809109530.4198762
GACAGGC3050.030.304131
TGGTACG500.005915989629.1576267
AGGATGG10500.029.1352169
AAGGATG9800.028.30584368
TCAGGTC9350.027.80540812
CGGACGC500.00753270927.75027545
TATCAAC28650.026.9956532
ATCAACG28700.026.9372883
TCAACGC28800.026.7238544
Produced by FastQC (version 0.11.5)