FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979087
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54540.55704957782097No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44600.45552642410735716No Hit
GCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAG44370.45317729680814883No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37370.38168211813659053No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTA33880.3460366647703422No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32680.33378034842664644No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG31320.319889856570458No Hit
GATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGT30990.3165193695759417No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28410.29016828943699585No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27470.28056750830110094No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG26650.27219235879957554No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26530.270966727165206No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26400.26963895956130557No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26140.2669834243535048No Hit
GTAATAATCAGCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG24700.25227584474107No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24420.24941603759420766No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24340.24859894983796127No Hit
CTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGT24140.24655623044734534No Hit
TCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCTCATAT23680.24185797584892862No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23290.23787467303722754No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23080.2357298176770808No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTT22830.23317641843881085No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22610.23092942710913328No Hit
GGATAGTTGCCTGCATATGAGCAGCAGTAATAATCAGCATCATCCTCAGC22560.23041874726147932No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22360.22837602787086336No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22300.22776321205367858No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22110.22582262863259342No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC21420.2187752467349684No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA21120.21571116764904447No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG20620.21060436917250458No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20440.20876592172095026No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20420.20856164978188865No Hit
GACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAGCAGTAATAAT20280.20713174620845745No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC19450.19865446073740128No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19270.1968160132858469No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATCACGAGTTTGGGGACTTTGCCTGGGTGCTGTTGGTACCAGGAGA18900.1930369824132074No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18640.19038144720540667No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18520.1891558155710371No Hit
GGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCG18210.18598960051558236No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17800.18180202576481966No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGC17740.18118920994763488No Hit
CAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17480.17853367473983417No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16740.17097561299455513No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTC16550.16903502957346997No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16130.16474531885317648No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCACTAAG16020.1636218231883377No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15830.16168123976725257No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15670.16004706425475979No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15460.15790220889461304No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA15330.15657444129071266No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15300.15626803338212028No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC15170.15494026577821993No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15160.15483812980868913No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15080.15402104205244274No Hit
GCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCC15000.15320395429619635No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAG14980.15299968235713476No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14810.1512633708751112No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14580.14891424357590285No Hit
GACCATCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCT13950.14247967749546261No Hit
ACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGC13820.14115190989156223No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGG13820.14115190989156223No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13600.1389049185618847No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13570.13859851065329232No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13570.13859851065329232No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13250.13533015962830677No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGGCCTCCCTGACCATCTCTGGCCTCCAGG13250.13533015962830677No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12980.13257248845097525No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12900.13175540069472888No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12770.1304276330908285No Hit
GTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCA12590.12858918563927413No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12580.12848704966974334No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12460.12726141803537377No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12430.12695501012678137No Hit
TCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC12410.12675073818771979No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT12240.1250144267056962No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12230.12491229073616542No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG12160.12419733894944984No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12080.12338025119320348No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT12070.12327811522367267No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT12060.12317597925414187No Hit
GTTATAACCACCAACATCACCGCTGGTTCCAGTGCAGGAGATGGTGACTG11900.12154180374164911No Hit
GAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATC11820.12072471598540271No Hit
GTCCAGGAGACCCGGACACTGAGCGAGGCTGAGTCAGGGCAGACTGAGCC11670.11919267644244078No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC11660.11909054047290997No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG11530.1177627728690096No Hit
GCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTG11470.11714995705182482No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC11410.11653714123464001No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11370.11612859735651683No Hit
CCTGAGGGCCGCTTAGTGACATCATAAATCACGAGTTTGGGGACTTTGCC11330.11572005347839365No Hit
ATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTT11200.11439228587449327No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11090.11326879020965451No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGA11080.11316665424012372No Hit
GGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTC11010.11245170245340812No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10970.11204315857528493No Hit
GTCCCCAAACTCGTGATTTATGATGTCACTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT10940.11173675066669254No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10690.1091833514284226No Hit
GCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCC10580.10805985576358382No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC10450.10673208815968346No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG10370.10591500040343707No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10330.10550645652531389No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10310.10530218458625229No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT10300.1052000486167215No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10300.1052000486167215No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTC10290.1050979126471907No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10260.10479150473859832No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10150.10366800907375953No Hit
GCAGTAATAATCAGCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGG10080.10295305728704396No Hit
GTCTGGCATCACGGCCTCCCTGACCATCTCTGGCCTCCAGGCTGAGGATG10040.10254451340892076No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC10030.10244237743938997No Hit
GGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGA9970.10182956162220518No Hit
CTCCCTGACCATCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATCC9960.10172742565267437No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC9850.10060392998783561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)