FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979087
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51650.5275322826265695No Hit
GCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAG42320.43223942305433527No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40930.4180425232895545No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39720.4056840709763279No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38990.3982281452005797No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTCCCTA32100.3278564621938602No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG30270.30916557976972425No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29720.30354810144553035No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28980.29599003970025134No Hit
GATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGT28710.29323236852291984No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27090.2766863414589306No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26770.2734179904339451No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26060.26616633659725847No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG23910.24420710314813696No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23670.24175583987939786No Hit
CTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGT23570.24073448018408988No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23420.23920244064112792No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22420.22898884368804814No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC22160.22633330848024744No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22050.22520981281540864No Hit
GTAATAATCAGCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG21980.22449486102869304No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21920.22388204521150826No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21820.2228606855162003No Hit
TCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCTCATAT21780.2224521416380771No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20860.21305563244124376No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTT20730.21172786483734338No Hit
GGATAGTTGCCTGCATATGAGCAGCAGTAATAATCAGCATCATCCTCAGC20660.21101291305062778No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG19820.2024334916100408No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC19650.2006971801280172No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19620.20039077221942486No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19560.19977795640224003No Hit
GACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGCCTGCATATGAGCAGCAGTAATAAT19460.19875659670693208No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA19330.1974288291030317No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19100.19507970180382336No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18840.1924241665960226No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18530.1892579515405679No Hit
GGATGATGCTGATTATTACTGCTGCTCATATGCAGGCAACTATCCCGTCG18470.18864513572338312No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18190.18578532857652078No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATCACGAGTTTGGGGACTTTGCCTGGGTGCTGTTGGTACCAGGAGA17470.17843153877030335No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17350.1772059071359338No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17060.17424396401954065No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16260.16607308645707686No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15750.16086415201100615No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGC15610.159434248437575No Hit
CAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA15560.15892356858992102No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15520.15851502471179782No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15450.15780007292508225No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACATTCAGCTTGGTC15010.15330609026572717No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCACTAAG15000.15320395429619635No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14930.15248900250948078No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA14880.1519783226618268No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14710.1502420111798032No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC14660.14973133133214925No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14550.14860783566731048No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT14330.1463608443376329No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14150.14452239688607857No Hit
GCATCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCC14120.14421598897748616No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13710.14002841422672346No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAG13590.1388027825923539No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12960.13236821651191366No Hit
GACCATCTCTGGCCTCCAGGCTGAGGATGATGCTGATTATTACTGCTGCT12540.12807850579162017No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGG12440.12705714609631216No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12240.1250144267056962No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12180.12440161088851144No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12110.12368665910179587No Hit
GTCCAGGAGACCCGGACACTGAGCGAGGCTGAGTCAGGGCAGACTGAGCC12070.12327811522367267No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11980.12235889149789549No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11940.1219503476197723No Hit
GTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTCA11940.1219503476197723No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC11840.12092898792446431No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11820.12072471598540271No Hit
ACCTAGGACATTCAGCTTGGTCCCTACGCCGAAAACGACGGGATAGTTGC11800.12052044404634113No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT11790.12041830807681034No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACACTGAGCGAGG11780.12031617210727953No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT11520.11766063689947881No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11400.11643500526510922No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11320.11561791750886286No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT11260.11500510169167807No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCATCACGGCCTCCCTGACCATCTCTGGCCTCCAGG11140.11377947005730848No Hit
TCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACC11130.11367733408777769No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11080.11316665424012372No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTC10960.11194102260575413No Hit
GTCCCCAAACTCGTGATTTATGATGTCACTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGT10760.10989830321513819No Hit
GTTATAACCACCAACATCACCGCTGGTTCCAGTGCAGGAGATGGTGACTG10750.10979616724560738No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT10660.10887694351983021No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC10510.10734490397686824No Hit
CCTGAGGGCCGCTTAGTGACATCATAAATCACGAGTTTGGGGACTTTGCC10460.10683422412921427No Hit
GCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTGATGCCAGACTTGGAGCC10370.10591500040343707No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC10330.10550645652531389No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10160.10377014504329032No Hit
GCTCATATGCAGGCAACTATCCCGTCGTTTTCGGCGTAGGGACCAAGCTGAATGTCCTAGGTCAGCCCAAGGCTG10160.10377014504329032No Hit
GGTCAGGGAGGCCGTGATGCCAGACTTGGAGCCAGAGAAGCGATCAGGGA10100.10315732922610554No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG10030.10244237743938997No Hit
GGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGATTTATGATGTC9960.10172742565267437No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9940.10152315371361278No Hit
CTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGTCCCCAAACTCGTGA9860.1007060659573664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)