FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852742
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30170.35379985974655875No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29700.3482882278578984No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA26290.30829957947421377No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA22890.26842819985411764No Hit
GTCTGGGACAGAGTTCATTCTCACCATCACCAGACTGGAGCCTGAAGATT21540.2525969167696677No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21260.2493133913891892No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20860.2446226408456485No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18440.21624360005722715No Hit
CTCCCAGACTCCTCATCCATGGTGCATCCACCAGGGCCACCGGCACCCCA18390.21565725623928456No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18330.21495364365775346No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCGGTGGCCCTGGT17920.21014562435062423No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17700.20756571155167683No Hit
GTAATACATGGCCGTGTCTTCGACTGTCAGGCTGTACATTTCAAGATGAA17540.20568941133426052No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17250.20228861719019353No Hit
GTCCTGTGCTGCGTCTGGGTTCAGCGTCGGCACCAACTACATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG17130.20088139202713132No Hit
GGCCAAAGGTGTACGGCAATGAAGAACCATATTGGTGACAGTAGTAAACT16920.19841874799177242No Hit
GAGTTCATTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTTTA16510.19361072868464319No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15910.1865746028693321No Hit
CTCCAGGCACTCTGTCCTTGTCTCCAGGAGAAACGGCCACCCTCTCCTGC15630.1832910774888536No Hit
GTTTACTACTGTCACCAATATGGTTCTTCATTGCCGTACACCTTTGGCCA15310.17953847705402104No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15140.17754490807301623No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14990.17578587661918846No Hit
ATGTATTACTGTGCAAGAGATATGCGGGGGTGGGGTGTTGATGGGGAGTA14760.17308869505665253No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14600.17121239483923625No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13720.16089274364344666No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13690.16054093735268113No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13640.1599545935347385No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13580.15925098095320742No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13580.15925098095320742No Hit
GAGTGTAACCTCCAAATATTTAAGTTGGTACCAGCAGAAACCTGGCCAAA13300.1559674555727289No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13190.1546774991732552No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12890.1511594362655997No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAACCTCCAAATATTTAAGTTGGTACC12840.1505730924476571No Hit
GTAGTAAACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAATGAACTCTGTCCCAGACCCACTGCCA12820.15033855492048004No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTG12760.14963494233894895No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12750.14951767357536042No Hit
GTCTCCAGGCACTCTGTCCTTGTCTCCAGGAGAAACGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTAACCTC12500.14658595448564748No Hit
GTCTGGGAGTTTGGCCAGGTTTCTGCTGGTACCAACTTAAATATTTGGAG11960.1402534412518675No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC11910.1396670974339249No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11890.13943255990674788No Hit
GTCCTTGTCTCCAGGAGAAACGGCCACCCTCTCCTGCAGGGCCAGTCAGA11780.1381426035072742No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11420.13392092801808753No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11340.13298277790937937No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11330.13286550914579087No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC11000.12899563994736976No Hit
TCCATGGTGCATCCACCAGGGCCACCGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCATT11000.12899563994736976No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10980.12876110242019276No Hit
GAATGAACTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCG10850.127236608493542No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10820.12688480220277645No Hit
CCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCATTCTCACC10800.12665026467559942No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10390.12184224536847019No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10360.12149043907770463No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10040.11773783864287206No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9980.11703422606134095No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9910.11621334471622131No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9890.11597880718904427No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9720.11398523820803946No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9640.11304708809933134No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9450.11081898159114949No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9410.11034990653679543No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9390.11011536900961838No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9280.10882541261014468No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9230.10823906879220209No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9190.10776999373784803No Hit
CAGTAATACATGGCCGTGTCTTCGACTGTCAGGCTGTACATTTCAAGATG9060.10624549981119728No Hit
CTATTACGGCTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCTCCAGCATCCCCGACCAGCCCCAAGGT9040.10601096228402027No Hit
TCACCAGACTGGAGCCTGAAGATTTTGCAGTTTACTACTGTCACCAATAT8760.10272743690354176No Hit
GCCGTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGG8710.10214109308559916No Hit
CTGCTACTCTGGCTCCCAGAAACCACCGGAGATATTGTGTTGACGCAGTC8580.10061659915894842No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACCGGAGATATTGTGTTGACGCAGTCTC8580.10061659915894842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)