FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004687914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852742
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31770.37256286192072163No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24820.2910610712267016No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA23720.27816150723196464No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21720.25470775451426103No Hit
GTCTGGGACAGAGTTCATTCTCACCATCACCAGACTGGAGCCTGAAGATT21610.2534177981147874No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA21290.2496651976799548No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21280.24954792891636626No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19240.2256251011443086No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19060.22351426339971528No Hit
GAGTTCATTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTTTA17990.21096650569574385No Hit
GTAATACATGGCCGTGTCTTCGACTGTCAGGCTGTACATTTCAAGATGAA17800.208738399187562No Hit
CTCCCAGACTCCTCATCCATGGTGCATCCACCAGGGCCACCGGCACCCCA17410.2041649174076098No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCGGTGGCCCTGGT16790.1968942540651217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16750.19642517901076761No Hit
GGCCAAAGGTGTACGGCAATGAAGAACCATATTGGTGACAGTAGTAAACT16440.19278984733952356No Hit
GTCCTGTGCTGCGTCTGGGTTCAGCGTCGGCACCAACTACATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16380.19208623475799244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15930.18680914039650914No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15700.18411195883397324No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14620.17144693236641328No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14560.17074331978488216No Hit
GTTTACTACTGTCACCAATATGGTTCTTCATTGCCGTACACCTTTGGCCA14320.16792886945875773No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14270.16734252564081514No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14160.16605256924134146No Hit
CTCCAGGCACTCTGTCCTTGTCTCCAGGAGAAACGGCCACCCTCTCCTGC13960.1637071939695711No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13860.16253450633368594No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTG13690.16054093735268113No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13490.15819556208091076No Hit
GAGTGTAACCTCCAAATATTTAAGTTGGTACCAGCAGAAACCTGGCCAAA13390.15702287444502558No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13200.15479476793684374No Hit
GTAGTAAACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAATGAACTCTGTCCCAGACCCACTGCCA13070.153270274010193No Hit
ATGTATTACTGTGCAAGAGATATGCGGGGGTGGGGTGTTGATGGGGAGTA12940.15174578008354228No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12720.14916586728459488No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12430.14576507314052786No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12080.14166066641492972No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11950.140136172488279No Hit
GTCCTTGTCTCCAGGAGAAACGGCCACCCTCTCCTGCAGGGCCAGTCAGA11860.13908075361598232No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11670.13685264710780048No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11640.13650084081703492No Hit
GTCTGGGAGTTTGGCCAGGTTTCTGCTGGTACCAACTTAAATATTTGGAG11440.13415546554526459No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAACCTCCAAATATTTAAGTTGGTACC11370.13333458420014493No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11270.13216189656425978No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAC11180.1311064776919631No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10890.1277056835478961No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10850.127236608493542No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10810.12676753343918795No Hit
GTCTCCAGGCACTCTGTCCTTGTCTCCAGGAGAAACGGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTAACCTC10600.12430488940382906No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10580.12407035187665202No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10460.1226631267135898No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC10410.12207678289564722No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10240.12008321391464241No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9960.1167996885341639No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9800.11492338831674762No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9770.11457158202598207No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9760.11445431326239355No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9560.11210893799062319No Hit
GAATGAACTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGGTGCCG9150.10730091868349394No Hit
TCCATGGTGCATCCACCAGGGCCACCGGCACCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCATT9150.10730091868349394No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9130.10706638115631692No Hit
CCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCATTCTCACC9090.10659730610196284No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8980.10530734970248913No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8880.10413466206660396No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCACCGGAGATATTGTGTTGACGCAGTCTC8800.10319651195789582No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8780.10296197443071878No Hit
CTGCTACTCTGGCTCCCAGAAACCACCGGAGATATTGTGTTGACGCAGTC8690.10190655555842212No Hit
GTATTACTGTGCAAGAGATATGCGGGGGTGGGGTGTTGATGGGGAGTATCACTATTACGGCTTGGACGTCTGGGG8580.10061659915894842No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8560.1003820616317714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)