FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584521
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT52290.8945786378932494No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC22280.38116680153493204No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCG19230.32898732466412667No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13160.22514161167862232No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12380.211797352019859No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11660.19947957387330822No Hit
CCCTCATGGGGGGCAAGGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAAGATGAGGCTGACTATTACTGCTTG11460.19605796883259968No Hit
GACTATTACTGCTTGCTCTCCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTCGGTCAG11380.19468932681631626No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11110.19007016001135973No Hit
CCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10580.18100290665348207No Hit
GTAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG10510.17980534488923408No Hit
CTATTACTGCTTGCTCTCCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTCGGTCAGCC10470.17912102388109238No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10150.17364645581595872No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGT9940.17005377052321474No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC9910.16954052976710846No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9570.16372380119790395No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT9460.16184191842551424No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA9400.16081543691330166No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9350.15996003565312453No Hit
CCCGAATACCGCTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGG9260.15842031338480567No Hit
AGGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAAGATGAGGCTGACTAT9200.1573938318725931No Hit
GTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTCGGTCAGCCC8860.15157710330338858No Hit
GCCTGAAGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAG8760.1498663007830343No Hit
GGGCAGCCTTGCCCCCCATGAGGGAGCCTGAAAACCGGGCAGGTGTCGAGGAATGTTTATTTCTTGTTTCATAAA8470.1449049734740069No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT8310.1421676894414401No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8310.1421676894414401No Hit
CACCAGTGTGGCCTTGTTGGCTAGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8150.13943040540887325No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCCC8070.13806176339258983No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8070.13806176339258983No Hit
TCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCCCCCATGAG8060.13789068314055442No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7910.135324479360023No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7800.1334425965876333No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7750.13258719532745616No Hit
CCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTC7690.1315607138152436No Hit
CTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCC7630.13053423230303104No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7620.1303631520509956No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCGCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC7530.12882342978267675No Hit
CAGTAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCCCCCATGAGGGAGC7370.1260861457501099No Hit
CCATGAGGGAGCCTGAAAACCGGGCAGGTGTCGAGGAATGTTTATTTCTTGTTTCATAAACCAATGTCCTGGGGA7360.1259150654980745No Hit
GGACATTGGTTTATGAAACAAGAAATAAACATTCCTCGACACCTGCCCGG7280.12454642348179107No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7270.12437534322975564No Hit
CTCCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCC6920.11838753440851568No Hit
CCCGAAAGGGTCAGGGCAGCCTTGCCCCCCATGAGGGAGCCTGAAAACCGGGCAGGTGTCGAGGAATGTTTATTT6880.11770321340037398No Hit
GGGCAAGGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAAGATGAGGCTGACTATTACTGCTTGCTCTCCTATAG6760.11565025037594885No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC6610.11308404659541745No Hit
CTCCAGCACTGGTGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGC6520.11154432432709861No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6220.10641191676603577No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6220.10641191676603577No Hit
GCCCTGACCCTTTCGGGTGCGCAGCCTGAAGATGAGGCTGACTATTACTG6150.1052143550017878No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6090.10418787348957523No Hit
GGTCAGGGCAGCCTTGCCCCCCATGAGGGAGCCTGAAAACCGGGCAGGTG6080.1040167932375398No Hit
GACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGTGCTGTCACCAGTGGTCATTATCC5970.1021349104651501No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5930.1014505894570084No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTAGAAGCTCCTCAGAGGA5910.10110842895293753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)