FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584521
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88801.5191926380745946No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC22760.3893786536326325No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCG17930.30674689189952115No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13970.23899911209349195No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13270.22702349445101203No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11180.19126772177560772No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11090.18972799950728886No Hit
GACTATTACTGCTTGCTCTCCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTCGGTCAG10740.18374019068604894No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA10730.18356911043401347No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10380.17758130161277355No Hit
CCCTCATGGGGGGCAAGGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAAGATGAGGCTGACTATTACTGCTTG10310.17638373984852554No Hit
CTATTACTGCTTGCTCTCCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTCGGTCAGCC10270.17569941884038384No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10160.17381753606799413No Hit
GTAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG9970.17056701127932103No Hit
CCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9680.16560568397029363No Hit
GGGCAGCCTTGCCCCCCATGAGGGAGCCTGAAAACCGGGCAGGTGTCGAGGAATGTTTATTTCTTGTTTCATAAA9540.16321056044179763No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT9470.16201299867754965No Hit
GTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTCGGTCAGCCC9080.155340868848168No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT8830.1510638625472823No Hit
GCCTGAAGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAG8710.14901089952285718No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8490.14524713397807779No Hit
CCCGAATACCGCTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGG8440.14439173271790065No Hit
AGGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAAGATGAGGCTGACTAT8330.14250984994551094No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCTAGCCAACAAGGCCACACTGGTGT8330.14250984994551094No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8250.14114120792922752No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7780.13310043608356245No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7680.13138963356320818No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7660.1310474730591373No Hit
TCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCCCCCATGAG7500.12831018902657046No Hit
CTCCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCC7390.12642830625418078No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCGCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC7390.12642830625418078No Hit
GGACATTGGTTTATGAAACAAGAAATAAACATTCCTCGACACCTGCCCGG7100.12146697894515339No Hit
CTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCC7090.12129589869311795No Hit
CAGTAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCCTTGCCCCCCATGAGGGAGC7070.1209537381890471No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6900.11804537390444483No Hit
CCATGAGGGAGCCTGAAAACCGGGCAGGTGTCGAGGAATGTTTATTTCTTGTTTCATAAACCAATGTCCTGGGGA6850.1171899726442677No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6830.11684781214019685No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC6780.1159924108800197No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCCCCGCCGAACAC6550.11205756508320489No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6550.11205756508320489No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6540.11188648483116946No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCCC6530.11171540457913404No Hit
CCTATAGCGGTATTCGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTC6490.11103108357099231No Hit
GGGCAAGGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAAGATGAGGCTGACTATTACTGCTTGCTCTCCTATAG6460.11051784281488604No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6170.10555651550585865No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6150.1052143550017878No Hit
CCCGAAAGGGTCAGGGCAGCCTTGCCCCCCATGAGGGAGCCTGAAAACCGGGCAGGTGTCGAGGAATGTTTATTT6140.10504327474975235No Hit
CACCAGTGTGGCCTTGTTGGCTAGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6100.10435895374161065No Hit
CTCCAGCACTGGTGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGC5940.10162166970904382No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC5850.10008194744072496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)