FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142073
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAACCTAAGTAGTTCTTATTGTTGAAGTCGTATAAAAGAGT63030.5518911663264957No Hit
CTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATCCGGGGTCCCT59620.5220331800156383No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53890.4718612558041386No Hit
ATATTATACGATTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT49500.433422381931803No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43230.3785222135537746No Hit
CTTTTATACGACTTCAACAATAAGAACTACTTAGGTTGGTACCAACAGAA41790.3659135624430312No Hit
GTATAATATTGGTGACAGGAATAAACTGCCACATCTTCAGCCTGCAGATTGTTGATGGTGAGAGAGAAATGTGTC40940.3584709558846063No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39270.34384842299923035No Hit
CTCTAACCCTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37260.32624884749048444No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32840.2875472933866749No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31710.2776530046678277No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30910.2706481984951925No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACCCTCTCCC30680.26863431672055993No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30620.2681089562576123No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29120.25497494468392123No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28770.25191034198339335No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACCCTCTCCCCTGTTGAAG28450.2491084195143393No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28170.24665673735391694No Hit
GGAATAAACTGCCACATCTTCAGCCTGCAGATTGTTGATGGTGAGAGAGAAATGTGTCCCAGATCCGTTGGCA27530.2410528924158088No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26980.2362370881721221No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25130.2200384738979032No Hit
GTTTATTCCTGTCACCAATATTATACGATTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA25030.2191628731263238No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG24890.21793703204611264No Hit
GTCCCTGACCGATTCAGTGCCAACGGATCTGGGACACATTTCTCTCTCAC23850.2088307840216869No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23770.20813030340442337No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23460.20541594101252722No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23250.20357717939221048No Hit
CTATTCGACAATCCGGGGTCCCTGACCGATTCAGTGCCAACGGATCTGGG23180.2029642588521049No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22320.19543409221652205No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCGAGTCCAGCCAGACTCTTTTATA22260.19490873175357443No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGTGACTACGGGTGCCTACGGGGACATCGTG22070.19324509028757356No Hit
GTATAAAAGAGTCTGGCTGGACTCGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA21680.1898302472784139No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCGAGTCCAGCCAGACTCTTTTATACGACTTCAA21660.18965512712409802No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21470.18799148565809717No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21400.18737856511799159No Hit
ACTTAGGTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGATCATT21210.18571492365199072No Hit
GTCGAATAGAAGCCCAATAAATGATCAGTTTAGGAGGCTGTCCTGGTTTC20890.18291300118293666No Hit
ATTCAGTGCCAACGGATCTGGGACACATTTCTCTCTCACCATCAACAATC20760.18177472017988341No Hit
CATTTCTCTCTCACCATCAACAATCTGCAGGCTGAAGATGTGGCAGTTTATTCCTGTCACCAATATTATACGATT20660.18089911940830403No Hit
GTCAGGGACCCCGGATTGTCGAATAGAAGCCCAATAAATGATCAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGTA20640.18072399925398813No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCGAGTCCAGC20360.1782723170935658No Hit
GAATCGGTCAGGGACCCCGGATTGTCGAATAGAAGCCCAATAAATGATCAGTTTAGGAGGCTGTCCTGGTTT20270.17748427639914438No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACCCTCTCCC19980.1749450341615641No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19650.17205555161535208No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19410.1699541097635615No Hit
GACCGATTCAGTGCCAACGGATCTGGGACACATTTCTCTCTCACCATCAA18650.1632995438995581No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18110.15857129973302933No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17810.15594449741829114No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16690.14613776877660184No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCGAGTCCAGCCAGACTCTTTTATACGACTTCAACAATAAGAACTA16680.1460502086994439No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16630.14561240831365418No Hit
AACCAGGACAGCCTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATCCGGGGTCCCTGACCGATTCAGTG16620.14552484823649625No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16520.14464924746491686No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16410.14368608661617951No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16390.14351096646186365No Hit
GTCCTGGTTTCTGTTGGTACCAACCTAAGTAGTTCTTATTGTTGAAGTCG16300.1427229257674422No Hit
CTTCTCCCTCTAACCCTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16280.1425478056131263No Hit
CCTAAGTAGTTCTTATTGTTGAAGTCGTATAAAAGAGTCTGGCTGGACTC16160.14149708468723105No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15960.13974588314407224No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15860.13887028237249283No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15840.13869516221817693No Hit
TCTTCAGCCTGCAGATTGTTGATGGTGAGAGAGAAATGTGTCCCAGATCCGTTGGCACTGAATCGGTCAGGGACC15580.13641860021207053No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15490.13563055951764905No Hit
CTACTTAGGTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATC15420.13501763897754346No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15330.134229598283122No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15040.13169035604554175No Hit
ATATTGGTGACAGGAATAAACTGCCACATCTTCAGCCTGCAGATTGTTGA14810.12967647427090911No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14770.12932623396227735No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14740.12906355373080355No Hit
GGATCTCTGTGACTACGGGTGCCTACGGGGACATCGTGCTGACCCAGTCT14620.12801283280490827No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14610.1279252727277503No Hit
CTGTGACTACGGGTGCCTACGGGGACATCGTGCTGACCCAGTCTCCAGAC14500.126962111879013No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT14380.1259113909531177No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14180.1241601894099589No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14170.12407262933280096No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14090.12337214871553745No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14060.12310946848406364No Hit
CCATCAACTGCGAGTCCAGCCAGACTCTTTTATACGACTTCAACAATAAG14040.12293434832974776No Hit
GCACTGAATCGGTCAGGGACCCCGGATTGTCGAATAGAAGCCCAATAAAT13960.12223386771248422No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13790.12074534640079924No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13750.12039510609216748No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13680.11978218555206191No Hit
GTACCAACAGAAACCAGGACAGCCTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATCCGGGGTCCCTGA13330.116717582851534No Hit
GTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC13300.1164549026200602No Hit
ACCATCAACTGCGAGTCCAGCCAGACTCTTTTATACGACTTCAACAATAA13290.11636734254290226No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13220.11575442200279668No Hit
ATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGAATCGTATAATATTG13190.11549174177132285No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACCCTCTCCCCTGTT13110.11479126115405931No Hit
GTATCAACGCAGAGTACGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGA13100.1147037010769014No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12930.1132151797652164No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12810.11216445883932114No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12730.1114639782220576No Hit
GTGCCAACGGATCTGGGACACATTTCTCTCTCACCATCAACAATCTGCAGGCTGAAGATGTGGCAGTTTATTC12490.10936253637026704No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12240.10717353444131855No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC12210.10691085420984474No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGTGGAATCGTATAATATTGGTGACAGGAATAAACTGCCACATCTTCAGC12110.10603525343826532No Hit
GGCTGAAGATGTGGCAGTTTATTCCTGTCACCAATATTATACGATTCCAC12000.10507209258952799No Hit
GGTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATCCGGGGTC11820.10349601120068508No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11770.10305821081489537No Hit
GCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGG11730.10270797050626361No Hit
GAGAGAAATGTGTCCCAGATCCGTTGGCACTGAATCGGTCAGGGACCCCG11670.10218261004331596No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGAATCGT11650.1020074898890001No Hit
GAACTACTTAGGTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGA11620.10174480965752628No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11600.1015696895032104No Hit
CACCAATATTATACGATTCCACTCACTTTCGGCGGAGGGACCAAGGTGGA11500.10069408873163098No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11450.1002562883458413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1550.053.28209740
CTTATAC2000.052.4570851
CACGAGA2000.050.79606221
CGTATGC1600.049.56341641
GGTATCA9050.047.866741
CCCACGA2150.047.23145719
CGAGACA2250.046.6693923
ATGCCGT1650.046.20478444
ACGAGAC2150.045.6770822
TTATACA2300.045.6148572
ATCTCGT1300.044.77419737
ATCTCCG2300.044.13954510
GCCCACG2400.042.30595418
TCTCCGA2400.042.29854611
TATACAC2500.041.9656683
CGAGCCC2450.041.43530715
GTATGCC2000.039.72161542
CCGAGCC2600.039.04310214
TCCGAGC2650.038.3064413
AATCTCG1550.037.50602736
Produced by FastQC (version 0.11.5)