FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142073
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATCCGGGGTCCCT58890.5156412943831086No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAACCTAAGTAGTTCTTATTGTTGAAGTCGTATAAAAGAGT55210.48341918598898664No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52550.4601282054649746No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42920.3758078511618784No Hit
ATATTATACGATTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT42910.37572029108472055No Hit
CTTTTATACGACTTCAACAATAAGAACTACTTAGGTTGGTACCAACAGAA37970.33246561296869814No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37700.3301014908854338No Hit
GTATAATATTGGTGACAGGAATAAACTGCCACATCTTCAGCCTGCAGATTGTTGATGGTGAGAGAGAAATGTGTC36120.3162669986944792No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32910.28816021392678054No Hit
CTCTAACCCTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30100.26355583224539936No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACCCTCTCCC29990.26259267139666204No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28910.25313618306360447No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27870.24402993503917875No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26800.23466100678327917No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26090.22844424130506544No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25580.2239786773700105No Hit
GGAATAAACTGCCACATCTTCAGCCTGCAGATTGTTGATGGTGAGAGAGAAATGTGTCCCAGATCCGTTGGCA24700.21627339058011177No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGTGACTACGGGTGCCTACGGGGACATCGTG24580.2152226696542165No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24350.2132087878795839No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACCCTCTCCCCTGTTGAAG24140.21137002625926715No Hit
GTTTATTCCTGTCACCAATATTATACGATTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA24040.21049442548768776No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23700.2075173828643178No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23270.20375229954652635No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22780.19946185576578732No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21570.18886708642967656No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21560.18877952635251863No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21480.1880790457352551No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21390.18729100504083362No Hit
GTCCCTGACCGATTCAGTGCCAACGGATCTGGGACACATTTCTCTCTCAC21320.18667808450072806No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21200.18562736357483278No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCGAGTCCAGCCAGACTCTTTTATA21080.1845766426489375No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20780.1819498403341993No Hit
CTATTCGACAATCCGGGGTCCCTGACCGATTCAGTGCCAACGGATCTGGG20600.18037375894535637No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCGAGTCCAGCCAGACTCTTTTATACGACTTCAA19720.17266847215545766No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19580.1714426310752465No Hit
ATTCAGTGCCAACGGATCTGGGACACATTTCTCTCTCACCATCAACAATC19510.1708297105351409No Hit
ACTTAGGTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGATCATT19270.16872826868335036No Hit
CATTTCTCTCTCACCATCAACAATCTGCAGGCTGAAGATGTGGCAGTTTATTCCTGTCACCAATATTATACGATT18550.16242394312797867No Hit
GTCAGGGACCCCGGATTGTCGAATAGAAGCCCAATAAATGATCAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGTA18300.16023494119903017No Hit
GTCGAATAGAAGCCCAATAAATGATCAGTTTAGGAGGCTGTCCTGGTTTC18160.15900910011881902No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18030.1578708191157658No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCGAGTCCAGC17880.1565574179583967No Hit
GACCGATTCAGTGCCAACGGATCTGGGACACATTTCTCTCTCACCATCAA17840.15620717764976497No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17840.15620717764976497No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17700.1549813365695538No Hit
GAATCGGTCAGGGACCCCGGATTGTCGAATAGAAGCCCAATAAATGATCAGTTTAGGAGGCTGTCCTGGTTT17520.15340525518071085No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17050.14928993155428769No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16840.14745116993397095No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16720.14640044900807567No Hit
GTATAAAAGAGTCTGGCTGGACTCGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16330.14298560599891602No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16190.14175976491870484No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCGAGTCCAGCCAGACTCTTTTATACGACTTCAACAATAAGAACTA16120.14114684437859926No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACCCTCTCCC16070.14070904399280956No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16050.14053392383849367No Hit
GGATCTCTGTGACTACGGGTGCCTACGGGGACATCGTGCTGACCCAGTCT16040.14044636376133574No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15850.1387827222953349No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15770.13808224167807137No Hit
AACCAGGACAGCCTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATCCGGGGTCCCTGACCGATTCAGTG15490.13563055951764905No Hit
GTATCAACGCAGAGTACGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGA15210.13317887735722672No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15050.13177791612269968No Hit
CTACTTAGGTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATC14710.12880087349932973No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14650.1282755130363821No Hit
GTCCTGGTTTCTGTTGGTACCAACCTAAGTAGTTCTTATTGTTGAAGTCG14350.12564871072164388No Hit
CTGTGACTACGGGTGCCTACGGGGACATCGTGCTGACCCAGTCTCCAGAC14340.12556115064448595No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14210.12442286964143273No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14100.1234597087926954No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14050.12302190840690569No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13950.12214630763532627No Hit
ATATTGGTGACAGGAATAAACTGCCACATCTTCAGCCTGCAGATTGTTGA13630.11934438516627222No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13590.11899414485764044No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13240.11592954215711256No Hit
CTTCTCCCTCTAACCCTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13200.11557930184848078No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13130.1149663813083752No Hit
TCTTCAGCCTGCAGATTGTTGATGGTGAGAGAGAAATGTGTCCCAGATCCGTTGGCACTGAATCGGTCAGGGACC13090.11461614099974346No Hit
CCATCAACTGCGAGTCCAGCCAGACTCTTTTATACGACTTCAACAATAAG13000.11382810030532199No Hit
GTGCCAACGGATCTGGGACACATTTCTCTCTCACCATCAACAATCTGCAGGCTGAAGATGTGGCAGTTTATTC12970.11356542007384816No Hit
CCTAAGTAGTTCTTATTGTTGAAGTCGTATAAAAGAGTCTGGCTGGACTC12960.11347785999669023No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12960.11347785999669023No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12920.11312761968805846No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12920.11312761968805846No Hit
GGCTGAAGATGTGGCAGTTTATTCCTGTCACCAATATTATACGATTCCAC12910.11304005961090052No Hit
GTACCAACAGAAACCAGGACAGCCTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATCCGGGGTCCCTGA12900.11295249953374258No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGAATCGT12780.11190177860784731No Hit
ATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGAATCGTATAATATTG12730.1114639782220576No Hit
ACCATCAACTGCGAGTCCAGCCAGACTCTTTTATACGACTTCAACAATAA12720.11137641814489967No Hit
GGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC12710.11128885806774172No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12690.11111373791342585No Hit
GGTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGATCATTTATTGGGCTTCTATTCGACAATCCGGGGTC12690.11111373791342585No Hit
GCACTGAATCGGTCAGGGACCCCGGATTGTCGAATAGAAGCCCAATAAAT12540.10980033675605674No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12540.10980033675605674No Hit
GTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC12530.10971277667889881No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12360.10822425536721383No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12210.10691085420984474No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT12190.10673573405552886No Hit
GCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGG12100.10594769336110738No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11870.10393381158647476No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACCCTCTCCCCTGTT11570.10130700927173657No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11560.10121944919457863No Hit
GAACTACTTAGGTTGGTACCAACAGAAACCAGGACAGCCTCCTAAACTGA11440.10016872826868337No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11430.10008116819152542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.00803112450.618463
GTGGTAT3250.043.6575431
GGTATCA9550.041.3881341
TGGTATC3650.038.8493162
ACACTCG400.00217957635.75259857
AGACCGA400.002895416233.741216
GTATCAA20900.032.8127861
CTGCGCT450.00378366531.94756159
GACAGGT1750.030.886971
CTTGTAC553.7077093E-430.6778583
CCAGTAA655.0015654E-429.14903667
CGGTTGG500.007047831528.13096850
GGGCGTA606.170776E-428.121373
TATCAAC24050.028.0764272
GATCTTA500.007531435727.7511144
ATCAACG24700.027.5976523
GATCTGG31350.027.37803370
AACGCAG25500.027.2576056
TCAACGC25100.027.1542824
CAACGCA25300.027.0729875
Produced by FastQC (version 0.11.5)