FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300264
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGTGGCCTCCGGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAGACGAACTGTGGCTGCACCAT45420.3493136778377314No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA44110.33923880073585055No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42960.3303944429746574No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41120.31624347055674845No Hit
CTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT39720.30547642632573074No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31620.24318138470341408No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30180.23210671063722443No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28090.21603305174949086No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25660.19734453926279588No Hit
GTCTCCAGCCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGGAG25020.19242246190004492No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24710.19003833067746242No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24240.1864236801141922No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23600.18150160275144125No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT23280.17904056407006577No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22720.17473374637765868No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT22540.17334941211938498No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22040.1695040391797358No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21510.16542794386370768No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21130.16250546042957428No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA20830.1601982366657848No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20690.15912153224268302No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20560.15812173527837423No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAAGTGG20410.15696812339647948No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20320.15627595626734261No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19910.1531227504568303No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19730.1517384161985566No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18650.14343241064891438No Hit
ATGTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATC18570.1428171509785705No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT18230.14020229737960907No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGC18230.14020229737960907No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18160.13966394516805816No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18050.13881796312133535No Hit
ATAATAGACGGCCGTGTCTTCAGATCTCAGGCGGCTCAGCTCCATGTAGGCTGTGTCTGTGGATGTGTCCTCGGT18030.1386641482037494No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18010.1385103332861634No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17920.13781816615702658No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17160.13197319928875983No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17150.13189629182996684No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17080.13135793961841596No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16870.1297428829837633No Hit
GCCTACATGGAGCTGAGCCGCCTGAGATCTGAAGACACGGCCGTCTATTA16710.12851236364307556No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16690.1283585487254896No Hit
CTCTTGCAGGGCCAGTCAGAGTGTTAGGAGCTATGTAGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCT16350.12574369512652817No Hit
GTTTATTACTGTCAGCAGTATAATAAGTGGCCTCCGGTCACTTTCGGCCC16060.12351337882153161No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTGACCGGAGGCCA15770.12128306251653509No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15690.12066780284619123No Hit
GTGCAGCCACAGTTCGTCTGATATCCACTTTGGTCCCAGGGCCGAAAGTGACCGGAGGCCACTTATTATACTG15620.12012945063464035No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA15620.12012945063464035No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15570.11974491334067544No Hit
GTGATTTATCCGTGCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAG15460.11889893129395261No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA15290.1175915044944719No Hit
GCCCCAGACTGTGCCAGTTGGACCTGGGCGTGGGTGCCTGTAGCTGCTGCCACCAAGAAGAGGAACCTCCAGGTG15050.1157457254834403No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15040.1156688180246473No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14980.1152073732718894No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14690.11297705696689288No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14390.11066983320310336No Hit
GATAAATCACTGCGTGTGTGTCCGGAAACCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCC14230.10943931386241564No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14180.10905477656845072No Hit
GGAGAAATAGTGATGACACAGTCTCCAGCCACCCTGTCTCTGTCTCCAGG14120.10859333181569282No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACTGTGGTCCCTTGG14120.10859333181569282No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14010.10774734976897No Hit
CCCCTACAGAAGCCCCCCGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGTTCCTCTTCTTGGTGGCAGCAG13940.10720899755741911No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13910.10697827518104015No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13730.10559394092276644No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13730.10559394092276644No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13620.10474795887604363No Hit
GTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGGAGCTATGTAGCCTGGTA13260.10197929035949624No Hit
GGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTGGATGGGAGGCTTTGATCCTGAAGATGATAAAATAGTCTATG13250.10190238290070325No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13250.10190238290070325No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT13110.10082567847760147No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13040.10028732626605058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATCG150.0025230167.8155831
TACGTAT702.1343092E-549.8083870
GGTATCA9200.047.609151
CAGTGAT14000.043.83137570
AGACGTA1950.041.5285425
CGTATGC1350.040.85587341
CTTATAC1950.039.7400251
GACGTAG2050.039.5240726
GTATCAA24500.039.4685131
TCGTATG1600.038.67767340
ACGTAGA2150.037.71044527
CGAGACG2200.036.7810623
ACGAGAC2200.036.77256822
TCTCCGA2250.035.9388111
CGTAGAG2300.035.27963628
GAGACGT2400.033.7198624
TTATACA2400.033.6913342
AATCTCG2150.033.30096436
TCCGAGC2550.033.0345313
CCCACGA2450.033.01137519
Produced by FastQC (version 0.11.5)