FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845422
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34560.40878992976288764No Hit
GTATTACTGTCAACAATATGGTAACTCACCTCTCACTTTCGGCGGAGGGA31560.3733046928043036No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28760.34018513830962527No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23620.27938709898725134No Hit
GTGTATTACTGTCAACAATATGGTAACTCACCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA21650.25608512671778116No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20540.24295558904310507No Hit
GTAATATATGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGTAGATACA20330.24047162245600423No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17770.21019088691801255No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17270.20427668075824854No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA16150.1910288589603772No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15890.1879534717572999No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15710.18582435753978485No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14990.1773079006697247No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14860.17577020706818605No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14180.16772688669090705No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14100.16678061370534478No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14060.16630747721256367No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT13640.1613395440383619No Hit
ATATATTACTGTGCGAAACGTGAAGGTCAGGTGCCGGAATATGGGTATGGTCCCGACTACTTTGACTACTGGGGC13420.15873729332806574No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13390.1583824409584799No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12670.14986598408841975No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCCGCTTAGCCTGGTACC12530.14821000636368584No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12470.14750030162451414No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12150.14371520968226517No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11970.14158609546475015No Hit
GTGTTAGCAGCAGCCGCTTAGCCTGGTACCAGCGGAATCCTGGCCAGGCTCCCAGGCTCCTCATCTACGGAGCAT11760.13910212887764925No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11700.13839242413847758No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11190.1323599338555183No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAACAATATGGTAACTCA11080.13105880850037024No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11020.13034910376119854No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10890.1288114101596599No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10620.12561773883338737No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10570.12502631821741095No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10240.12112294215196671No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10230.12100465802877143No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT10130.11982181679681862No Hit
GCCTGGTACCAGCGGAATCCTGGCCAGGCTCCCAGGCTCCTCATCTACGGAGCATCCAACAGGGCCACTGGCATC10000.11828412319527999No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9920.11733785020971775No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9840.11639157722415552No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9790.1158001566081791No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9270.10964938220202455No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9190.10870310921646231No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9010.10657399499894728No Hit
CTCCTGTTCAGCCTCTGGATTCACTTTTAACATCAATGCCATGACCTGGGTCCGCCAGGTTCCAGGGAAGGGG8890.10515458552060392No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA8850.10468144902782278No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA8690.10278890305669831No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG8550.1011329253319644No Hit
GTCAGAGTGTTAGCAGCAGCCGCTTAGCCTGGTACCAGCGGAATCCTGGC8550.1011329253319644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACT200.0066951553.00082854
CCTAACG200.00780482650.9846462
GGTATCA8800.040.570371
GTGGTAT3050.040.1333161
GTATCAA21550.037.551911
TAGTTCG853.43789E-935.9827777
TGGTATC3350.035.5116962
AATGGTA2500.034.38313770
TCTGGAG9050.033.60867770
CGGTACC652.2042152E-532.08703245
TTAACGG751.7507882E-632.00209435
CGCGGTA652.2530012E-531.98446843
ATCAACG24750.031.855463
TATCAAC25450.031.2518862
TCAACGC25300.031.0286254
CAACGCA25650.030.6052325
AACGCAG26050.030.2657436
TAACCGT703.3189775E-530.22113853
ACCGCCT803.107547E-629.7322512
ACGCAGA27450.028.7221347
Produced by FastQC (version 0.11.5)