FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883739
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26130.2956755331608088No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24130.2730444169602111No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17960.20322742348136724No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16290.18433044145386818No Hit
GAAATAGACGGCCGTGTCCGCAGCGGTGACAGAGGTCAGTTTTAGGGAGAATTGATTGTTGGACGTGTCTACTAA16210.18342519680584424No Hit
GTCTCTGGTGACTCCATCAGTGGATACTACTGGACCTGGATCCGGCAGAC16120.18240679657681738No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15940.1803699961187636No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15750.1782200400797068No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15170.17165701638153347No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15080.17063861615250658No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14950.1691675935994677No Hit
CTACTGGACCTGGATCCGGCAGACCCCAGGGAAGGGACTGGAGTGGATTGGATATTTTTATTCCACTGGGACCAC14300.16181248083427346No Hit
CTCCTGATGTATGCATTCAATTTGCAAAATGGGGTCCCATCAAGGTTTAC14240.16113354734825555No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13670.1546836792310852No Hit
GGATATTTCCAGGTGGTTAGCATGGTATCAGCAGACACCAGGGAAAGCCC13540.15321265667804634No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13350.15106270063898955No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13070.1478943443709059No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13060.1477811887899029No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12970.14676278856087602No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12940.14642332181786705No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12750.14427336577881025No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12330.13952083137668472No Hit
GTATCAGCAGACACCAGGGAAAGCCCCGAAACTCCTGATGTATGCATTCA12320.13940767579568175No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11960.13533407487957416No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11900.13465514139355622No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGTGGATACTACTGGACCTGGATCCGGCAGACCCCAGGGAAGGGA11770.1331841188405174No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11670.1320525630304875No Hit
CATCAGGAGTTTCGGGGCTTTCCCTGGTGTCTGCTGATACCATGCTAACC11580.1310341628014606No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11580.1310341628014606No Hit
CTACAATCCCTCCCTCAGGAGTCGAGTCACCATGTTAGTAGACACGTCCAACAATCAATTCTCCCTAAAACTGAC10910.12345273887426039No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10640.12039753818717969No Hit
GTGTCAGCCTGTTGACAATAGTAAGTTGCAAAGTCTTCAGGCTGCAGGGTGCTGATGGTGAGAGTGAAATCTGTC10490.11870020447213488No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10330.11688971517608705No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCATATCCACTGCCGGTAAACCT10330.11688971517608705No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10290.1164370928520751No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10210.11553184820405119No Hit
GTCTTCAGGCTGCAGGGTGCTGATGGTGAGAGTGAAATCTGTCCCATATCCACTGCCGGTAAACCTTGATGGGAC9950.1125898030979735No Hit
GATTTCACTCTCACCATCAGCACCCTGCAGCCTGAAGACTTTGCAACTTACTATTGTCAACAGGCTGACACTTTC9860.1115714028689466No Hit
GTCTATTTCTGTGCGAAAGGAAGTCACTCAGCGATCAGCTGGGGCCAGGG9770.1105530026399197No Hit
CCCCAGGGAAGGGACTGGAGTGGATTGGATATTTTTATTCCACTGGGACC9760.11043984705891671No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9740.11021353589691074No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9700.10976091357289877No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9690.10964775799189579No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9590.1085162021818659No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT9560.10817673543885695No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCGCCGCCGAAAGTC9340.1056873126567912No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9300.10523469033277925No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9290.10512153475177627No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9280.10500837917077327No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9250.1046689124277643No Hit
GGCTGACACTTTCCCGCTGACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9150.10353735661773442No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9090.1028584231317165No Hit
GTTTTAGGGAGAATTGATTGTTGGACGTGTCTACTAACATGGTGACTCGA9050.10240580080770453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1400.054.31030341
TCGTATG1400.054.21672440
GACGTAG1700.054.0484526
AGACGTA1750.052.4863725
CGACACA200.007797685550.99664721
CGTAGAG1850.049.70274728
ACGTAGA1850.049.67740227
CGAGACG1900.048.31807723
ATCTCGT1600.047.22265237
AATCTCG1600.047.15525436
ACGAGAC2000.045.90217622
GAGACGT2050.044.79275524
ATCTCCG2000.044.1920910
CCCACGA2150.042.69003719
CCGTCTT1800.042.61401447
TATACAC2350.041.945193
CACGAGA2200.041.72452521
ATGCCGT1950.040.9410444
TAATACC350.001448773138.8523920
GCCCACG2450.038.8501918
Produced by FastQC (version 0.11.5)