FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883739
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23330.263991970479972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20700.23423205267618608No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18960.21454298158166607No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17200.19462759932514012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16940.19168555421906242No Hit
GTCTCTGGTGACTCCATCAGTGGATACTACTGGACCTGGATCCGGCAGAC15370.17392012800159323No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14460.1636229701303213No Hit
GAAATAGACGGCCGTGTCCGCAGCGGTGACAGAGGTCAGTTTTAGGGAGAATTGATTGTTGGACGTGTCTACTAA14300.16181248083427346No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14180.1604546138622376No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13970.15807834666117485No Hit
CTACTGGACCTGGATCCGGCAGACCCCAGGGAAGGGACTGGAGTGGATTGGATATTTTTATTCCACTGGGACCAC13960.15796519108017187No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13680.15479683481208817No Hit
CTCCTGATGTATGCATTCAATTTGCAAAATGGGGTCCCATCAAGGTTTAC13560.15343896784005231No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13170.14902590018093576No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13090.14812065553291187No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12430.14065238718671463No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12420.14053923160571163No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12320.13940767579568175No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12190.1379366532426429No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12170.13771034208063693No Hit
GGATATTTCCAGGTGGTTAGCATGGTATCAGCAGACACCAGGGAAAGCCC12070.13657878627060704No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11820.13374989674553234No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGTGGATACTACTGGACCTGGATCCGGCAGACCCCAGGGAAGGGA11310.12797896211437992No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11290.12775265095237395No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11150.1261684728183321No Hit
GTGCTGATGGTGAGAGTGAAATCTGTCCCATATCCACTGCCGGTAAACCT11120.12582900607532316No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11120.12582900607532316No Hit
GTATCAGCAGACACCAGGGAAAGCCCCGAAACTCCTGATGTATGCATTCA11120.12582900607532316No Hit
GTCTTCAGGCTGCAGGGTGCTGATGGTGAGAGTGAAATCTGTCCCATATCCACTGCCGGTAAACCTTGATGGGAC11080.1253763837513112No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11020.12469745026529326No Hit
GGCTGACACTTTCCCGCTGACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10960.12401851677927532No Hit
GTGTCAGCCTGTTGACAATAGTAAGTTGCAAAGTCTTCAGGCTGCAGGGTGCTGATGGTGAGAGTGAAATCTGTC10620.12017122702517372No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10530.11915282679614682No Hit
CTACAATCCCTCCCTCAGGAGTCGAGTCACCATGTTAGTAGACACGTCCAACAATCAATTCTCCCTAAAACTGAC10440.11813442656711992No Hit
CATCAGGAGTTTCGGGGCTTTCCCTGGTGTCTGCTGATACCATGCTAACC10380.11745549308110201No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10300.1165502484330781No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10200.1154186926230482No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10170.11507922588003924No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10130.1146266035560273No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10040.1136082033270004No Hit
GATTTCACTCTCACCATCAGCACCCTGCAGCCTGAAGACTTTGCAACTTACTATTGTCAACAGGCTGACACTTTC9860.1115714028689466No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCGCCGCCGAAAGTC9840.11134509170694062No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT9820.11111878054493465No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9790.11077931380192568No Hit
CCCCAGGGAAGGGACTGGAGTGGATTGGATATTTTTATTCCACTGGGACC9750.11032669147791373No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9720.10998722473490477No Hit
GTTTTAGGGAGAATTGATTGTTGGACGTGTCTACTAACATGGTGACTCGA9700.10976091357289877No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9490.10738464637183602No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9300.10523469033277925No Hit
GTCTATTTCTGTGCGAAAGGAAGTCACTCAGCGATCAGCTGGGGCCAGGG9180.10387682336074337No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9180.10387682336074337No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8960.10138740057867764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC200.00785981950.8943637
GTATCAA22000.038.2782061
GTGGTAT3200.038.2010351
TTAACGG601.4031866E-534.27678335
TATCAAC25400.032.7366072
ATCAACG25900.032.1009943
TCAACGC26150.031.7887024
CAACGCA26400.031.6161945
AACGCAG26700.031.3880336
TGTACTA553.5907928E-430.845075
ACGCAGA27500.030.4749287
CAGAGTA28350.029.91856810
AGAGTAC28450.029.57489611
CGCAGAG28250.029.5457538
TAACGGC703.9836625E-529.42383636
GCAGAGT28700.029.3172769
GCGAACT1401.6370905E-1129.19317828
TATAAGA1052.732122E-829.0907232
CCGTTAA704.305436E-529.09072316
TATACCA704.3137545E-529.0824935
Produced by FastQC (version 0.11.5)