FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031658
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA75510.7319286042467562No Hit
GTATATTACTGTGCGAGAGATTTCGGTGGAGGCTACTTTGACTATTGGGGCCAGGGAACCCTGGTCACCGTCTC47830.463622634632795No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG45790.4438486397624019No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG36860.3572889465307301No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35900.3479835371799569No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA35680.3458510475370714No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT32890.31880720161138676No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT28720.27838682974396556No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27810.26956607713021175No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26630.258128178136553No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG25450.24669027914289424No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT23730.23001808738942559No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21470.20811160287614694No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21380.20723922074951193No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT21190.20539752514883808No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT20730.20093868316825922No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20430.19803074274614263No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG19710.1910516857330627No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19670.19066396034344715No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGTCATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG19660.19056702899604325No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19580.18979157821681214No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC19270.18678670644729164No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT18630.18058310021344282No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT18610.18038923751863506No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCGGTT18470.1790321986549806No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18470.1790321986549806No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18030.17476721936920955No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG17890.17341018050555512No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTTTCAGC17760.1721500729893046No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16770.1625538695963197No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC16390.15887047839497198No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16350.1584827530053564No Hit
CTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC15900.1541208423721815No Hit
GCCTTAGCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGC15530.15053438251823764No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC15390.1491773436545832No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15260.14791723613833266No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG14770.14316760011554217No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAAGTAGC14560.14113204182006053No Hit
GCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG14540.14093817912525275No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14500.1405504537356372No Hit
CAGTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATA14490.14045352238823333No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC14300.13861182678755946No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT14210.13773944466092444No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13840.13415298480698062No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13750.1332806026803456No Hit
CCTTTAGTAGTCATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG13640.13221435785890284No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13640.13221435785890284No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13640.13221435785890284No Hit
TCTATGATGCATCCAACCGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13610.13192356381669118No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATGACTAC13550.13134197573226786No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC13460.13046959360563287No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTTTCAGCTGG13420.13008186821601733No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC13390.12979107417380567No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT12900.12504143815101515No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTCCTTAGCCTGGTA12820.12426598737178406No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC12770.12378133063476462No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCC12730.12339360524514909No Hit
CTCCAGAGACAACGCCGAGAACTCACTGTATCTGCAAATGAGCAGCCTGAGAGCCGAGGACACGGCTGTATATTA12710.1231997425503413No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT12670.12281201716072573No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTTGGCCGAACGTC12630.1224242917711102No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG12460.1207764588652441No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT12430.12048566482303245No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12410.12029180212822467No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA12380.12000100808601301No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC12350.11971021404380135No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12340.11961328269639744No Hit
GTGTGAGGTGCAGTCGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC12180.11806238113793524No Hit
ACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGTTGGCCAACATAAACCAAGATGAGAGTGTGAAAAA12100.11728693035870413No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA12070.11699613631649247No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG12030.11660841092687693No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA11980.11612375418985749No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGTCATTGG11980.11612375418985749No Hit
GTTTATTACTGTCAGCAGCGTTTCAGCTGGCGGACGTTCGGCCAAGGGAC11980.11612375418985749No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG11900.1153483034106264No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11900.1153483034106264No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA11880.11515444071581861No Hit
CATCTATGATGCATCCAACCGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11820.11457285263139529No Hit
GAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGT11790.11428205858918362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11790.11428205858918362No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT11710.11350660780995252No Hit
GGGTTCCCTGGCCCCAATAGTCAAAGTAGCCTCCACCGAAATCTCTCGCACAGTAATATACAGCCGTGTCCTCGG11330.10982321660860479No Hit
CCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTCACCTGCCGCGTGGATCACAGGGGC11320.10972628526120091No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACAGAGTCCACATAGTTTTTCACACTCTCATCTTGGTTTATGTTGGC11300.10953242256639313No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC11210.10866004043975815No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC11200.10856310909235424No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11160.1081753837027387No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11110.10769072696571927No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11070.1073030015761037No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTG11010.10672141349168038No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGTCGGTGGAGT10930.10594596271244927No Hit
GGGCTGGAGTGGTTGGCCAACATAAACCAAGATGAGAGTGTGAAAAACTA10920.10584903136504539No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10790.10458892384879485No Hit
CCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC10690.10361961037475596No Hit
GAGTGTTAGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC10550.10226257151110155No Hit
ATATACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACAGTG10540.10216564016369767No Hit
ACCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAG10530.10206870881629376No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10350.10032394456302379No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10350.10032394456302379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)