FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840197
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCACCTTCAGGGAGA34390.40930876925292525No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC25620.3049284870095942No Hit
GTCTCTGGTGGCTCCATGAGTAGTTACTACTGGACCTGGATCCGGCAGCC23930.2848141566799215No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23450.2791012107874701No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23080.2746974816620388No Hit
CTCCTGCACTGTCTCTGGTGGCTCCATGAGTAGTTACTACTGGACCTGGATCCGGCAGCCCCCCGGGAAGGGA22660.2696986540061438No Hit
GTGTATTACTGTGCGAGAGCTGGGGGCGCGCAGCAACTTGATTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC22240.2646998263502488No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19850.2362541165940845No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT18790.22363802774825425No Hit
CTATTACACTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCTTTAGACACGTCCAAGAA18550.22078155480202855No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17280.20566605212825084No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16700.1987629091748721No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16040.19090760857275138No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15530.18483760356202175No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15370.18293328826453797No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14740.1754350467806955No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14430.17174543589182062No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAA14320.1704362191248005No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14300.17019817971261503No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14110.167936805296853No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACAG14040.16710366735420384No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAG13850.16484229293844183No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13290.1581771893972485No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13100.1559158149814865No Hit
CAGTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCACCTTCAGGGA12760.1518691449743334No Hit
GTGTAATAGATATAGCCAATCCACTCCAGTCCCTTCCCGGGGGGCTGCCG12620.15020286908903507No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12610.15008384938294234No Hit
GGCTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCTAAGCTCCTGATCTTTGCTGCATC12470.148417573497644No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12470.148417573497644No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG12400.14758443555499484No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12140.14448992319658366No Hit
GTATTACTGTGCGAGAGCTGGGGGCGCGCAGCAACTTGATTCCTGGGGCC12090.14389482466611997No Hit
GGACTGGAGTGGATTGGCTATATCTATTACACTGGGAGCACCAACTACAA11840.14091933201380152No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11800.14044325318943057No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11710.13937207583459593No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11670.13889599701022498No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCTTTAGACACGTCCA11280.1342542284726082No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11230.13365912994214452No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG11190.13318305111777357No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT11110.13223089346903166No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11110.13223089346903166No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACAGGCTGACAGGTTC10900.12973147964108417No Hit
GACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10710.12747010522532215No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA10620.1263989278704875No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10610.1262799081643948No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10500.12497069139737467No Hit
CTACAACACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG10360.12330441551207634No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9980.1187816666805523No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9960.11854362726836681No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAATGTG9940.11830558785618135No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCC9830.11699637108916122No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC9800.11663931197088302No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT9800.11663931197088302No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9770.1162822528526048No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9610.114377937555121No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA9500.11306872078810089No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAATGTGATCGGGAAC9380.11164048431498803No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC9290.1105693069601534No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT9180.10926009019313328No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG9160.10902205078094782No Hit
CCGTGGGCATGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGCTGGG9080.10806989313220589No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8990.10699871577737126No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGATGTTCCCCTGCTGCCACCTGCTCTTGTCCACGGTGAG8960.10664165665909303No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC8660.10307106547631092No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8530.10152380929710533No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC8490.10104773047273437No Hit
ATTAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTCCCTAA8480.10092871076664163No Hit
GTCCCTAAGCTCCTGATCTTTGCTGCATCCGCTTTGCAAACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT8460.10069067135445617No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATT8410.10009557282399248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)