FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730609
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGG55720.7626514318876444No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGG55630.7614195828411641No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40670.5566588968928661No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39360.5387286496607625No Hit
GTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCC38550.5276420082424388No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36540.5001307128710432No Hit
GTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT30290.41458564019879307No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC29820.40815265073383983No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28440.389264298687807No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26200.3586049446420726No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25140.34409650031685896No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23810.3258925088522041No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23110.3163114607129121No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCAC22610.3094678548991321No Hit
GTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22150.30317173755045446No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21780.29810746924825726No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20590.28181968741146085No Hit
CTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20170.27607105852788566No Hit
GCCTTGGGCTGACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGACTGTCATCCCATG20110.27524982583023205No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19810.271143662341964No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGG19220.2630682074817036No Hit
GACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGA19080.26115199785384524No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19070.26101512573756963No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGAGGGTGACGTTGGTC18740.2564983459004748No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18060.247191041993734No Hit
GGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCCAAGGCCAACCC18040.2469172977611828No Hit
CTCTTGTTCTGGAAGCAATTCCAACATCGGAAGTAATTTTGTATCCTGGTACCAGCACCTCCCAGGAACGGCCCC17900.2450010881333244No Hit
TTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCA17050.23336695824989837No Hit
CCCCTGAGGGCCGCTGATCATTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATA16920.23158762073831554No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16560.22666022455239396No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16450.22515463127336233No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16370.22405965434315755No Hit
GTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCC15970.21858476969213356No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT15620.21379424562248756No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG15430.21119367541325113No Hit
CCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG15320.20968808213421955No Hit
ACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGAC15240.20859310520401475No Hit
CTTTAGCACTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGG14030.19203157913466712No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13790.1887466483440527No Hit
GGCCATCAGTGGCCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG13760.18833603199522592No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13110.17943934443731188No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCT13020.17820749539083147No Hit
CTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCC12940.17711251846062667No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12850.17588066941414626No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12810.17533318094904388No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12690.1736907155537367No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCG12370.16931080783291746No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12240.16753147032133467No Hit
GTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACC12040.16479402799582266No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12040.16479402799582266No Hit
CTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG11950.16356217894934225No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11950.16356217894934225No Hit
ATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11930.16328843471679105No Hit
GTATTATTGTGTAGGCTGGACAAGCCTCGCGGGATTTGACTACTGGGGCC11700.16014037604245226No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11650.15945601546107424No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11600.15877165487969627No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11580.15849791064714505No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGAC11000.15055932790316023No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAG10870.14877999039157744No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGAC10800.14782188557764822No Hit
GTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGAATTGCTTCCAG10750.14713752499627022No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10530.14412633843820705No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG10510.14385259420565583No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10470.14330510574055344No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCAC10440.14289448939172664No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTC10350.14166264034524623No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTC10250.14029391918249023No Hit
GACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAATTCCAACAT10160.13906207013600982No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10090.1381039653220806No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10030.13728273262442703No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9960.1363246278104978No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG9800.1341346739500882No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG9700.13276595278733222No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTT9700.13276595278733222No Hit
GTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGC9680.132492208554781No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCCGAGGATG9550.13071287104319823No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9550.13071287104319823No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGA9430.129070405647891No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA9350.12797542871768622No Hit
AGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGG9280.127017323903757No Hit
GTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAATTCCAACATCGGAAGTAATTTTGTATC9280.127017323903757No Hit
TCCTCGGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACC9240.1264698354386546No Hit
GTTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGA9200.1259223469735522No Hit
GTACCAGCACCTCCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGA9200.1259223469735522No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC9200.1259223469735522No Hit
GTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG9190.1257854748572766No Hit
CAATAATACAGAGCCGTGTCCTCATCTCTCAGGCTGTCCATTTGAAGATA9040.12373239311314259No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8990.1230480325317646No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8920.12208992771783539No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGA8910.12195305560155978No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG8830.120858078671355No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8820.1207212065550794No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8740.11962622962487458No Hit
TTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGAATTGCTTCCAGAACAAGAGATGGTGA8500.1163412988342602No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8480.11606755460170899No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC8370.11456196132267739No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGA8360.11442508920640178No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG8340.11415134497385059No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG8310.11374072862502377No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG8250.11291949592737018No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA8250.11291949592737018No Hit
GCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGT8240.11278262381109458No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG8230.11264575169481897No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8010.10963456513675579No Hit
CTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAG7900.10812897185772417No Hit
GTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGC7840.10730773916007057No Hit
GTCAGGGACCCCTGAGGGCCGCTGATCATTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTA7840.10730773916007057No Hit
TGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCCAAGGCCAAC7820.10703399492751936No Hit
GTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCT7810.10689712281124378No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA7800.10676025069496817No Hit
CCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCCGAGGATGAGGCTGAT7790.10662337857869257No Hit
TCAGTGGCCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGG7790.10662337857869257No Hit
GGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT7750.10607589011359017No Hit
ACGTCACCCTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG7750.10607589011359017No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC7740.10593901799731456No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGAGGGT7730.10580214588103898No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAG7690.10525465741593656No Hit
GGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCCT7690.10525465741593656No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGG7620.10429655260200738No Hit
GGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC7530.10306470355552697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7520.10292783143925135No Hit
GGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGT7490.10251721509042457No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG7490.10251721509042457No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT7450.10196972662532217No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCAC7440.10183285450904656No Hit
GCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGT7430.10169598239277097No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7380.10101162181139296No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT7340.10046413334629056No Hit
GGGTTGGCCTTGGGCTGACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGACTGTCATCC7320.10019038911373937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGCG150.002283789769.5377551
GTGGTAT1450.052.1019551
TGATCCG200.007176710852.07742349
TGGTATC1550.048.7171632
GCCAGAG21750.047.3885570
GGTATCA5300.047.2983861
GTATCAA13400.041.7716981
TATCAAC14650.038.189242
GTCCTAG451.0290023E-438.155471
TCCGCCC400.001596309238.09165268
GACAGGT754.1851308E-836.6292531
TACCACA1201.1456694E-535.29797770
ATCAACG15850.035.2737773
TCAACGC16150.034.6137964
GCTTGCC701.0090116E-634.3399241
GCTTGAT400.00265558934.3399241
GTACGGA400.00265558934.3399241
CCTCGGT400.002657374834.3352226
CAACGCA16350.033.980635
AACGCAG16550.033.5699846
Produced by FastQC (version 0.11.5)